A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of sp...A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of spectral analysis.展开更多
[ Objective] This study aimed to investigate the inhibitory effects of 95%, 70% ethanol extracts and water extract of Cordyceps militaris （Bombyx mori）, on cells infected with respiratory syncytial virus （RSV）. [M...[ Objective] This study aimed to investigate the inhibitory effects of 95%, 70% ethanol extracts and water extract of Cordyceps militaris （Bombyx mori）, on cells infected with respiratory syncytial virus （RSV）. [Method] Human embryonic kidney cells （HEK293, RSV virus-sensitive cells） were infected with respiratory syncytial virus and incubated with tissue cell culture method, to analyze the inhibitory effects of different extracts of Cordyceps militaris （Bombyx mori） on proliferation of RSV-infected cells by cytopathic effect （CPE） observation and tetrazolium salt reduction assay （ MTF method）. [ Result] Different extracts of Cordyceps militaris （Bombyx mori） were non-toxic to HEK293 cells. CPE occurred successively in various sample groups after 48 h, and the degree of CPE was reduced gradually with the increasing concentration. To be specific, 95 % ethanol extract （100 μg/ml）, 70% ethanol extract （100, 25 μg/ml） and water extract （100, 25 μg/ml） of Cordyceps militaris （BombTx mori） exhibited inhibitory effects against RSV subtype A; 70% ethanol extract （100, 25 μg/ml） and water extract （100, 25 μg/ml） of Cordyceps militaris （Bombyx mori） exhibited inhibitory effects against RSV subtype B; the effects of water extract （100 μg/ml） of Cordyceps militaris （Bombyx mori） on RSV subtypes A and B were similar to positive control group. However, different extracts of Cordyceps militaris （Bombyx mot/） with low concentrations （6.25, 1.56 μg/ml） exhibited no inhibitory effects against respiratory syncytial virus. [ Conclusion ] Cordyceps militaris （Bombyx mori） exhibited significant inhibitory effects against syncytial virus, and the inhibitory effects were related to the water soluble components.展开更多
A new angelol-type coumarin glucoside, 6-[（1R,2R）-1, 2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin, was isolated from the roots of Angelica pubescens. Its structure was elucidated on the basis of s...A new angelol-type coumarin glucoside, 6-[（1R,2R）-1, 2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin, was isolated from the roots of Angelica pubescens. Its structure was elucidated on the basis of spectral analysis.展开更多
A new neolignan glycoside, 4-O-β-d-glucopyranosyl-9-O-β-d-glucopyranosyl-(7R,8S)-dehydrodiconiferyl alcohol was isolated from the fresh roots of Angelica dahurica. The structure of the new compound was elucidated on...A new neolignan glycoside, 4-O-β-d-glucopyranosyl-9-O-β-d-glucopyranosyl-(7R,8S)-dehydrodiconiferyl alcohol was isolated from the fresh roots of Angelica dahurica. The structure of the new compound was elucidated on the basis of spectral analysis.展开更多
Two new triterpenoid saponins named lonimacranthoideⅣ（1） and lonimacranthoideⅤ（2） were isolated from the flower buds of Lonicera macranthoides Hand.-Mazz.（Caprifoliaceae）.They have hederagenin as aglycone.Loni...Two new triterpenoid saponins named lonimacranthoideⅣ（1） and lonimacranthoideⅤ（2） were isolated from the flower buds of Lonicera macranthoides Hand.-Mazz.（Caprifoliaceae）.They have hederagenin as aglycone.LonimacranthoideⅣ（1） is a rare chlorogenic acid ester acylated on the C-23 of hederagenin.LonimacranthoideⅣis a new sulfated triterpenoid saponin.The structures of the saponins were established by chemical and spectral methods.展开更多
文摘A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of spectral analysis.
基金Supported by National Natural Science Foundation of China(30973927)
文摘[ Objective] This study aimed to investigate the inhibitory effects of 95%, 70% ethanol extracts and water extract of Cordyceps militaris （Bombyx mori）, on cells infected with respiratory syncytial virus （RSV）. [Method] Human embryonic kidney cells （HEK293, RSV virus-sensitive cells） were infected with respiratory syncytial virus and incubated with tissue cell culture method, to analyze the inhibitory effects of different extracts of Cordyceps militaris （Bombyx mori） on proliferation of RSV-infected cells by cytopathic effect （CPE） observation and tetrazolium salt reduction assay （ MTF method）. [ Result] Different extracts of Cordyceps militaris （Bombyx mori） were non-toxic to HEK293 cells. CPE occurred successively in various sample groups after 48 h, and the degree of CPE was reduced gradually with the increasing concentration. To be specific, 95 % ethanol extract （100 μg/ml）, 70% ethanol extract （100, 25 μg/ml） and water extract （100, 25 μg/ml） of Cordyceps militaris （BombTx mori） exhibited inhibitory effects against RSV subtype A; 70% ethanol extract （100, 25 μg/ml） and water extract （100, 25 μg/ml） of Cordyceps militaris （Bombyx mori） exhibited inhibitory effects against RSV subtype B; the effects of water extract （100 μg/ml） of Cordyceps militaris （Bombyx mori） on RSV subtypes A and B were similar to positive control group. However, different extracts of Cordyceps militaris （Bombyx mot/） with low concentrations （6.25, 1.56 μg/ml） exhibited no inhibitory effects against respiratory syncytial virus. [ Conclusion ] Cordyceps militaris （Bombyx mori） exhibited significant inhibitory effects against syncytial virus, and the inhibitory effects were related to the water soluble components.
基金the Jiangsu Province Basic Facility Project(Nos.:BM2006104,BM2006507).
文摘A new angelol-type coumarin glucoside, 6-[（1R,2R）-1, 2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin, was isolated from the roots of Angelica pubescens. Its structure was elucidated on the basis of spectral analysis.
文摘A new neolignan glycoside, 4-O-β-d-glucopyranosyl-9-O-β-d-glucopyranosyl-(7R,8S)-dehydrodiconiferyl alcohol was isolated from the fresh roots of Angelica dahurica. The structure of the new compound was elucidated on the basis of spectral analysis.
基金supported by the National Key Technologies R&D Program of China during the 11th Five-Year Plan Period(No2009ZX09103-397)
文摘Two new triterpenoid saponins named lonimacranthoideⅣ（1） and lonimacranthoideⅤ（2） were isolated from the flower buds of Lonicera macranthoides Hand.-Mazz.（Caprifoliaceae）.They have hederagenin as aglycone.LonimacranthoideⅣ（1） is a rare chlorogenic acid ester acylated on the C-23 of hederagenin.LonimacranthoideⅣis a new sulfated triterpenoid saponin.The structures of the saponins were established by chemical and spectral methods.
