Different kinds of biological databases publicly available nowadays provide us a goldmine of multidiscipline big data.The Cancer Genome Atlas is a cancer database including detailed information of many patients with c...Different kinds of biological databases publicly available nowadays provide us a goldmine of multidiscipline big data.The Cancer Genome Atlas is a cancer database including detailed information of many patients with cancer.DrugBank is a database including detailed information of approved,investigational and withdrawn drugs,as well as other nutraceutical and metabolite structures.PubChem is a chemical compound database including all commercially available compounds as well as other synthesisable compounds.Protein Data Bank is a crystal structure database including X-ray,cryo-EM and nuclear magnetic resonance protein three-dimensional structures as well as their ligands.On the other hand,artificial intelligence(AI)is playing an important role in the drug discovery progress.The integration of such big data and AI is making a great difference in the discovery of novel targeted drug.In this review,we focus on the currently available advanced methods for the discovery of highly effective lead compounds with great absorption,distribution,metabolism,excretion and toxicity properties.展开更多
Enzalutamide(ENZ) is a second-generation androgen receptor(AR) antagonist used for the treatment of castration-resistant prostate cancer(CRPC) and reportedly prolongs survival time within a year of starting therapy. H...Enzalutamide(ENZ) is a second-generation androgen receptor(AR) antagonist used for the treatment of castration-resistant prostate cancer(CRPC) and reportedly prolongs survival time within a year of starting therapy. However, CRPC patients can develop ENZ resistance(ENZR), mainly driven by abnormal reactivation of AR signaling, involving increased expression of the full-length AR(ARfl)or dominantly active androgen receptor splice variant 7(ARv7) and ARfl/ARv7 heterodimers. There is currently no efficient treatment for ENZR in CRPC. Herein, a small molecule LLU-206 was rationally designed based on the ENZ structure and exhibited potent inhibition of both ARfl and constitutively active ARv7 to inhibit PCa proliferation and suppress ENZR in CRPC. Mechanically, LLU-206 promoted ARfl/ARv7 protein degradation and decreased ARfl/ARv7 heterodimers through mouse double minute 2-mediated ubiquitination. Finally, LLU-206 exhibited favorable pharmacokinetic properties with poor permeability across the blood-brain barrier, leading to a lower prevalence of adverse effects, including seizure and neurotoxicity, than ENZ-based therapies. In a nutshell, our findings demonstrated that LLU-206 could effectively inhibit ARfl/ARv7-driven CRPC by dual-targeting of ARfl/ARv7 heterodimers and protein degradation, providing new insights for the design of new-generation AR inhibitors to overcome ARfl/ARv7-driven CRPC.展开更多
The discovery of targeted drugs heavily relies on three-dimensional(3D)structures of target proteins.When the 3D structure of a protein target is unknown,it is very difficult to design its corresponding targeted drugs...The discovery of targeted drugs heavily relies on three-dimensional(3D)structures of target proteins.When the 3D structure of a protein target is unknown,it is very difficult to design its corresponding targeted drugs.Although the 3D structures of some proteins(the so-called undruggable targets)are known,their targeted drugs are still absent.As increasing crystal/cryogenicelectron microscopy structures are deposited in Protein Data Bank,it is much more possible to discover the targeted drugs.Moreover,it is also highly probable to turn previous undruggable targets into druggable ones when we identify their hidden allosteric sites.In this review,we focus on the currently available advanced methods for the discovery of novel compounds targeting proteins without 3D structure and how to turn undruggable targets into druggable ones.展开更多
基金This work was supported by NSFC(no 81872892 and no 2018ZX09735001-004)‘Double First Class'University project(no CPU2018GY20 and no CPU2018GY38).
文摘Different kinds of biological databases publicly available nowadays provide us a goldmine of multidiscipline big data.The Cancer Genome Atlas is a cancer database including detailed information of many patients with cancer.DrugBank is a database including detailed information of approved,investigational and withdrawn drugs,as well as other nutraceutical and metabolite structures.PubChem is a chemical compound database including all commercially available compounds as well as other synthesisable compounds.Protein Data Bank is a crystal structure database including X-ray,cryo-EM and nuclear magnetic resonance protein three-dimensional structures as well as their ligands.On the other hand,artificial intelligence(AI)is playing an important role in the drug discovery progress.The integration of such big data and AI is making a great difference in the discovery of novel targeted drug.In this review,we focus on the currently available advanced methods for the discovery of highly effective lead compounds with great absorption,distribution,metabolism,excretion and toxicity properties.
基金supported by the National Natural Science Foundation of China (Nos. 81903656 and 81673468)the National Key New Drug Innovation Program, the Ministry of Science and Technology of China (No. 2018ZX09201017-006)+3 种基金Natural Science Foundation of Jiangsu Province (No. BK20180560, China)China Postdoctoral Science Foundation (No. 2018M632430)“Double First-Class” University project (Nos. CPU2018GF10 and CPU2018GY46, China)the Scientific Startup Foundation for High level Scientists of China Pharmaceutical University (No. 3154070026, China)
文摘Enzalutamide(ENZ) is a second-generation androgen receptor(AR) antagonist used for the treatment of castration-resistant prostate cancer(CRPC) and reportedly prolongs survival time within a year of starting therapy. However, CRPC patients can develop ENZ resistance(ENZR), mainly driven by abnormal reactivation of AR signaling, involving increased expression of the full-length AR(ARfl)or dominantly active androgen receptor splice variant 7(ARv7) and ARfl/ARv7 heterodimers. There is currently no efficient treatment for ENZR in CRPC. Herein, a small molecule LLU-206 was rationally designed based on the ENZ structure and exhibited potent inhibition of both ARfl and constitutively active ARv7 to inhibit PCa proliferation and suppress ENZR in CRPC. Mechanically, LLU-206 promoted ARfl/ARv7 protein degradation and decreased ARfl/ARv7 heterodimers through mouse double minute 2-mediated ubiquitination. Finally, LLU-206 exhibited favorable pharmacokinetic properties with poor permeability across the blood-brain barrier, leading to a lower prevalence of adverse effects, including seizure and neurotoxicity, than ENZ-based therapies. In a nutshell, our findings demonstrated that LLU-206 could effectively inhibit ARfl/ARv7-driven CRPC by dual-targeting of ARfl/ARv7 heterodimers and protein degradation, providing new insights for the design of new-generation AR inhibitors to overcome ARfl/ARv7-driven CRPC.
基金supported by NSFC(No.81872892 and No.2018ZX09735001-004)“Double First-Class”University project(No.CPU2018GY20 and No.CPU2018GY38).
文摘The discovery of targeted drugs heavily relies on three-dimensional(3D)structures of target proteins.When the 3D structure of a protein target is unknown,it is very difficult to design its corresponding targeted drugs.Although the 3D structures of some proteins(the so-called undruggable targets)are known,their targeted drugs are still absent.As increasing crystal/cryogenicelectron microscopy structures are deposited in Protein Data Bank,it is much more possible to discover the targeted drugs.Moreover,it is also highly probable to turn previous undruggable targets into druggable ones when we identify their hidden allosteric sites.In this review,we focus on the currently available advanced methods for the discovery of novel compounds targeting proteins without 3D structure and how to turn undruggable targets into druggable ones.