Development and evaluation of a RT-RAA-combined CRISPR/Cas12a assay for the detection of African horse sickness virus

African horse sickness(AHS)is an acute and fatal vectorborne infectious disease of equids,caused by the African horse sickness virus(AHSV).The World Organization for Animal Health(WOAH)has classified AHS as a notifiable animal disease,and AHS has also been classified as a Class I animal infectious disease in China.AHS is mainly found in Africa,the Middle East and the Arabian Peninsula.China is currently recognized by the WOAH as an AHS-free zone.

Design and Research on Identification of Typical Tea Plant Diseases Using Small Sample Learning

Tea plants are susceptible to diseases during their growth.These diseases seriously affect the yield and quality of tea.The effective prevention and control of diseases requires accurate identification of diseases.With the development of artificial intelligence and computer vision,automatic recognition of plant diseases using image features has become feasible.As the support vector machine(SVM)is suitable for high dimension,high noise,and small sample learning,this paper uses the support vector machine learning method to realize the segmentation of disease spots of diseased tea plants.An improved Conditional Deep Convolutional Generation Adversarial Network with Gradient Penalty(C-DCGAN-GP)was used to expand the segmentation of tea plant spots.Finally,the Visual Geometry Group 16(VGG16)deep learning classification network was trained by the expanded tea lesion images to realize tea disease recognition.

FvWRKY50 is an important gene that regulates both vegetative growth and reproductive growth in strawberry

The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberry which played essential roles in strawberry vegetative growth,and reproductive growth.Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit.Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering-and leaf senescence-related genes,including FvFT2,FvCO,FvFT3,and FvSAUR36.Notably,FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions,but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination,delaying anthocyanin accumulation.In addition,the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size.These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.

Host niche, genotype, and field location shape the diversity and composition of the soybean microbiome

Plant-associated microbes represent a key determinant of plant fitness through acquiring nutrients,promoting growth,and resisting to abiotic and biotic stresses.However,an extensive characterization of the bacterial and fungal microbiomes present in different plant compartments of soybean in field conditions has remained elusive.In this study,we investigated the effects of four niches(roots,stems,leaves,and pods),four genotypes(Andou 203,Hedou 12,Sanning 16,and Zhonghuang 13),and three field locations(Jining,Suzhou,and Xuzhou)on the diversity and composition of bacterial and fungal communities in soybean using 16S and internal transcribed spacer rRNA amplicon sequencing,respectively.The soybean microbiome significantly differed across organs.Host genotypes explained more variation in stem bacterial community composition and leaf fungal community composition.Field location significantly affected the composition of bacterial communities in all compartments and the effects were stronger in the root and stem than in the leaf and pod,whereas field location explained more variation in stem and leaf fungal community composition than in the root and pod.The relative abundances of potential soybean fungal pathogens also differed among host organs and genotypes,reflecting the niches of these microbes in the host and probably their compatibility to the host genotypes.Systematic profiling of the microbiome composition and diversity will aid the development of plant protection technologies to benefit soybean health.

Compressive properties of glue-laminated timber circular post modified by basalt fiber reinforced polymer

Glued timber structure is one of the main forms of modern wood architecture,which has gradually developed towards mid-and high-rise buildings.Glue-laminated timber(GLT)is comprised of several laminates of parallel-to-grain dimension lumber that are bonded together with durable,moisture resistant structural adhesives.GLT can be used in horizontal applications as a beam and in vertical applications as a post.So,its compressive performance has a significant impact on structural safety.Fiber reinforced polymers(FRPs)were commonly used to improve the bearing capacity of GLT components,and the structural and process parameters largely determined the reinforcement effect.This study was aimed at investigating the influence of structural and process parameters on the axial compression performance of GLT components.Three wrapping methods(middle-part,end-part and full wrapping)and three lengths(0.6,0.8,and 1.0 m)of wood post specimens were designed in this work and the axial compression performance and ductility of GLT post specimens modified by basalt fiber reinforced polymer(BFRP)were studied.The results showed that the effect of different BFRP wrapping methods on the compressive strength and elastic modulus of laminated wood was not statistically significant(P>0.05).The compressive bearing capacity of unreinforced GLT posts decreased with the increase of aspect ratio.The GLT posts with middle-part and end-part wrapping still followed this pattern,while the compressive bearing capacity of GLT posts with full wrapping showed a pattern of first decreasing and then increasing.For GLT with low aspect ratios(4.0 or 5.3),there was no correlation between the wrapping method and the compressive bearing capacity,while the compressive bearing capacity of GLT with a high aspect ratio(6.7)for middle-part,end-part and full wrapping increased by 3.5%,7.5%and 9.7%,respectively.Compared to the unreinforced group,the ultimate axial compressive strength and modulus of elasticity(MOE)of the 6-E series specimens reinforced at both ends decreased by 2.58%and 6.70%,respectively.The ultimate axial compressive strength of the 8-E specimens reinforced at both ends increased by 8.62%and the MOE decreased by 1.91%.The compressive strength of the 10-E specimens reinforced at both ends increased by 7.51%and the MOE increased by 8.14%.The failure modes of GLT with different aspects were consistent under the same BFRP wrapping,while the failure modes of GLT with the same aspect ratio were different for different BFRP wrapping methods.The ductility performance of GLT with different aspects ratio was improved by the BFRP wrapping.

A Study on the Influence of Network Service Platforms on Increasing Income of Strawberry Growers in the Context of New Media

With the rapid development of new media,the role of network service platforms in various fields is becoming increasingly prominent.In the agricultural field,network service platforms under the background of new media have brought new opportunities and challenges to strawberry growers.This paper conducted in-depth research on the impact of network service platforms on strawberry growers'income in the context of new media.Firstly,the concepts and characteristics of new media and network service platforms,as well as the theoretical basis of farmers'income increase,were explained.Then,the current application of network service platforms in strawberry cultivation was analyzed,including the cognition and use of the platform by strawberry growers,and the service content provided by the platform,such as market information push,technical guidance and training,and sales channel expansion.The influence mechanism of network service platforms on strawberry growers'income was deeply discussed,mainly reflected in reducing information asymmetry,enhancing product added value,and expanding sales channels.In summary,the network service platform under the background of new media provides strong support for strawberry growers'income increase,but still needs to be continuously improved and developed to better serve agricultural production and farmers'income increase.

Effects of soilless substrates on seedling quality and the growth of transplanted super japonica rice

This study was conducted to investigate the effects of soilless substrates of hydroponically grown long-mat seedlings （HLMS） on seedling quality and field growth characteristics of transplanted super japonica rice. A widely grown conventional super japonica rice cultivar （Wuyunjing 23） was selected as the test material. The effect of HLMS on seedling quality, mechanical transplantation quality, field growth characteristics, yield, and benefit-cost ratio were compared with seedlings grown in organic substrates and traditional nutritive soil, whJch was selected as the control. Root number, root twJstJng power and root activity of seedlings cultivated by HLMS were decreased compared to that of the organic substrates andcontrol. However, seedling root length as well as aboveground growth were increased compared to the organic substrates and control seed- lings. In the HLMS, the content of gibberellin acid （GA3） decreased while abscisic acid （ABA） content increased compared to that of the organic substrates and control seedlings. During the early stages after transplanting, the re-greening of HLMS was delayed compared to that of the organic substrates and control seedlings. Nevertheless, there were no significant differences in tiller dynamics and crop yield among the HLMS, organic substrates and control treatments. The effects of HLMS on seedling production were similar to those of the organic substrates and traditional nutritive soil in the present study, suggesting that HLMS have the potential to replace traditional nutritive soil in seedling production without decreasing crop yield. Finally, it is important to reduce organic substrates and topsoil dependence during rice seedling production and worthwhile to consider HLMS popularization and its application on a larger scale.

Development of Rural E-commerce from the Perspective of Agricultural New Kinetic Energy Cultivation:Based on Survey on the "E-commerce Entering Villages and Households" Project of Jurong City

Taking the implementation of the"e-commerce entering villages and households"project in Jurong City as an example,a survey plan is formulated,and questionnaires are designed.Through questionnaires,a large number of agricultural households are visited,and the data are collected and collated;and using interviews and field trips,the help of e-commerce for agricultural products sales is understood.Then,the analysis on the entire survey is summarized.Finally,the survey is researched,and it is found that the current rural e-commerce lacks professional talents and the product distribution system is not perfect.For existing problems,corresponding solutions are put forward.

A research on the path of rural culture protection and tourism utilization using memetics

Through the review and reflection on the protection of rural culture,this paper thinks that there are many factors affecting the protection of rural culture,which need to be analyzed systematically.This paper takes the cultural meme theory as the basic theory of rural cultural protection and inheritance,and constructs a set of factors affecting the protection of rural culture from two aspects of external environment and group psychology.Based on 335 questionnaires of rural tourism destination residents in Jiangsu Province,this paper deeply analyzes the influence of rural cultural protection and its driving factors,and uses structural equation model to calculate.The empirical results show that the group psychological factors as an intermediate variable,physical environmental factors and social environmental factors significantly affect the group psychological factors,thus affecting the rural cultural protection.At the same time,the impact of cultural and tourism utilization factors on rural cultural protection is also significant.From the perspective of the main body of rural cultural protection,this paper explores the psychology of the main body of rural cultural protection,promotes the upgrading of rural tourism quality and efficiency while protecting rural culture,deepens theoretical research and strengthens practical application,which has important enlightenment significance for realizing the healthy inheritance of rural culture.On the other hand,this paper can provide reasonable suggestions for the governance and development of rural traditional tourism.

Spatio-temporal Changes and Associated Uncertainties of CENTURYmodelled SOC for Chinese Upland Soils, 1980-2010

Detailed information on the spatio-temporal changes of cropland soil organic carbon(SOC) can significantly contribute to the improvement of soil fertility and mitigate climate change. Nonetheless, information and knowledge on the national scale spatio-temporal changes and the corresponding uncertainties of SOC in Chinese upland soils remain limited. The CENTURY model was used to estimate the SOC storages and their changes in Chinese uplands from 1980 to 2010. With the Monte Carlo method, the uncertainties of CENTURY-modelled SOC dynamics associated with the spatial heterogeneous model inputs were quantified. Results revealed that the SOC storage in Chinese uplands increased from 3.03(1.59 to 4.78) Pg C in 1980 to 3.40(2.39 to 4.62) Pg C in 2010. Increment of SOC storage during this period was 370 Tg C, with an uncertainty interval of –440 to 1110 Tg C. The regional disparities of SOC changes reached a significant level, with considerable SOC accumulation in the Huang-Huai-Hai Plain of China and SOC loss in the northeastern China. The SOC lost from Meadow soils, Black soils and Chernozems was most severe, whilst SOC accumulation in Fluvo-aquic soils, Cinnamon soils and Purplish soils was most significant. In modelling large-scale SOC dynamics, the initial soil properties were major sources of uncertainty. Hence, more detailed information concerning the soil properties must be collected. The SOC stock of Chinese uplands in 2010 was still relatively low, manifesting that recommended agricultural management practices in conjunction with effectively economic and policy incentives to farmers for soil fertility improvement were indispensable for future carbon sequestration in these regions.

Effects of soil fauna on leaf litter decomposition under different land uses in eastern coast of China

Soil fauna decompose litter, whereas land use changes may significantly alter the composition and structure of soil fauna assemblages. However, little is known of the effects of land-use on the contribution of soil fauna to litter decomposition. We studied the impacts of soil fauna on the decomposition of litter from poplar trees under three different land uses （i.e. poplar-crop integrated system, poplar plantation, and cropland）, from December 2013 to December 2014, in a coastal area of Northern Jiangsu Province. We collected litter samples in litterbags with three mesh sizes （5, 1 and 0. 01 mm, respectively） to quantify the contribution of various soil fauna to the decomposition of poplar leaf litter. Litter decomposition rates differed significantly by land use and were highest in the cropland, intermediate in the poplar-crop integrated system, and lowest in the poplar plantation. Soil fauna in the poplar-crop integrated system was characterized by the highest numbers of taxa and individuals, and highest Margalef＇s diversity, which suggested that agro-forestry ecosystems may support a greater quantity, distribution, and biodiversity of soil fauna than can single-species agriculture or plantation forestry. The individuals and groups of soil fauna in the macro-mesh litterbags were higher than in the meso-mesh litterbags underthe same land use types. The average contribution rate of meso- and micro-fauna to litter decomposition was 18.46%, which was higher than the contribution rate of macro-fauna （3.31%）. The percentage of remaining litter mass was inversely related to the density of the soil fauna （P 〈 0.05） in poplar plantations; however, was unrelated in the poplar-crop integrated system and cropland. This may have been the result of anthropogenic interference in poplar-crop integrated systems and croplands. Our study suggested that when land-use change alters vegetation types, it can affect species composition and the structure of soil fauna assemblages, which, in turn, affects litter decomposition.

The Origin of PRRSV and Advances in the Evolution and Pathogenicity of PRRSV 1

Porcine reproductive and respiratory syndrome virus(PRRSV) has been mutating and evolving constantly since its emergence in the1980s, which has brought inestimable economic losses to the global swine industry. The virus has two genotypes, of which genotype 1 PRRSV(PRRSV 1) first broke out in Germany and mainly prevailed in Europe, which can be clustered into four subtypes based on the ORF5 sequence. Al-though few cases of PRRSV 1 have been reported in China, the prevention and control of PRRSV should not be ignored. The origin of PRRSV, ge-netic evolution and pathogenicity of PRRSV 1 were retrospectively analyzed, in order to provide valuable evidences for molecular epidemiology and immune prevention and control of PRRSV 1.

Research on Homestay Construction in Huanglongxian Tea Culture Village from the Perspective of Culture and Tourism Integration

Against the background of COVID-19 pandemic,tourists have had increasing demands on rural tourism,and characteristic homestay has won great popularity.Huanglongxian tea culture homestay in Jiangning District,Nanjing City has been in the development stage.Literature analysis,questionnaire survey,interview and experience method were used to analyze the development status and tourist needs of Huanglongxian tea homestay.The research showed that Huanglongxian tea homestay was confined by inconvenient traffic,serious homogeneity,singular experience projects,and unremarkable tea culture theme;The main tourists were the locals,particularly middle-aged and young groups of enterprises and public institutions with higher educational backgrounds,most of whom visited the village during holidays for relaxation and recreation,and the revisit rate was low.In view of this,it was proposed that the integration of tea culture and homestay should be enhanced,more characteristic homestay products of tea culture developed,tea homestay market expanded,tea homestay brands built,so as to promote the development of tea homestay tourism.

Research on key parameters of the Fatigue Arousal Zone in extra-long tunnels based on natural driving experiments

To effectively mitigate the short-term fatigue effects of driving in extra-long tunnels,this study conducted natural driving experiments in five extra-long tunnels of varying lengths and tunnel group sections.Utilizing data obtained from natural driving fatigue experiments,this study identified perclos P80,variable coefficient of pupil diameter,and acceleration as fatigue sensitivity indicators,determined through significance tests of difference and correlation analysis.This study employed an ordered multi-class Logistic model to investigate the factors that influence driving fatigue in extra-long tunnels.The most significant variable in the model was perclos P80,which served as an indicator for classifying and identifying fatigue levels in extra-long tunnels.Following this,a dimensionless quantitative metric,the Fatigue Driving Degree,was formulated,and the Threshold of Driving Fatigue was established.Using the quantitative framework for driving fatigue,this paper standardized the definition of the fatigue arousal zone in extra-long tunnels.The study analyzed the operational principles and validated the key parameters of the fatigue arousal zone in extra-long tunnels.These parameters encompass the placement location,length,form,and traffic induction design of the fatigue arousal zone.The research findings can serve as a theoretical reference for the development of fatigue arousal technology in extra-long highway tunnels in China.

Evaluation of Water Transfer Capacity of Poplar with Pectinase Treated under the Solar Interface Evaporation

Poplar wood,which was used as the absorption material for the solar-driven interfacial evaporation,was treated for 3 days,6 days and 9 days with the pectinase,and then was simulated for photothermal evaporation test at one standard solar radiation intensity(1 kW⋅m^(−2)).The effects of pectinase treatment on cell passage and water migration capacity of poplars were analyzed by the mercury intrusion porosimetry,the scanning electron microscope and fractal theory.It was found that the pit membrane and the ray parenchyma cells of poplar wood were degraded and destroyed after pectinase treatment.Compared with the untreated poplar wood,the evaporation rate of three sections of the specimen was changed.Especially the evaporation rate of radial and tangential direction was significantly increased.At the same time,based on the experimental data and fractal dimension deduction,fractal characteristics could be found in that the structure of poplars treated with pectinase.The porosity decreased with the increase of the fractal dimension in a certain range.It was shown that it is feasible to evaluate solar-driven water migration capacity by using fractal theory.

加拿大西部铁杉正交胶合木胶合性能与耐久性初探

以加拿大西部所产铁杉锯材为基材,采用单组分聚氨酯(PUR)和双组分异氰酸酯(EPI)两种不同胶黏剂,0.28 MPa和0.83 MPa两个不同压力参数,制造正交胶合木(CLT)小样(609 mm×609 mm),对其进行胶层剪切测试和浸渍剥离测试,分析判断胶黏剂种类和压力对铁杉CLT胶合性能和耐久性的影响。试验结果表明:胶黏剂种类和压力大小对cLT的胶合性能、剪切破坏中木材破坏率和耐久性均有显著影响:当使用单组分聚氨酯(PUR)、压力0.83 MPa时,相比于0.28 MPa,其胶层剪切试验中木材破坏率更高;浸渍剥离试验中胶层剥离率更低。虽然在北美现行标准中尚没有利用加拿大铁杉制造CLT的相关规定,但加拿大铁杉的各项性能符合CLT制造的要求。建议进行进一步研究,制定合理的工艺参数,将加拿大丰富的铁杉资源用于制造CLT。

Species diversity, updated classification and divergence times of the phylum Mucoromycota

Zygomycetes are phylogenetically early diverging,ecologically diverse,industrially valuable,agriculturally beneficial,and clinically pathogenic fungi.Although new phyla and subphyla have been constantly established to accommodate spe-cific members and a subkingdom Mucoromyceta,comprising Calcarisporiellomycota,Glomeromycota,Mortierellomycota and Mucoromycota,was erected to unite core zygomycetous fungi,phylogenetic relationships within phyla have not been well resolved.Taking account of the information of monophyly and divergence time estimated from ITS and LSU rDNA sequences,the present study updates the classification framework of the phylum Mucoromycota from the class down to the generic rank:three classes,three orders,20 families(including five new families Circinellaceae,Protomycocladaceae,Rhizomucoraceae,Syzygitaceae and Thermomucoraceae)and 64 genera.The taxonomic hierarchy was calibrated with estimated divergence times:phylum earlier than 617 Mya,classes and orders earlier than 547 Mya,families earlier than 199 Mya,and genera earlier than 12 Mya.Along with this outline,all genera of Mucoromycota are annotated and 58 new species are described.In addition,three new combinations are proposed.In this study,we update the taxonomic backbone of the phylum Mucoromycota and reinforce its phylogeny.We also contribute numerous new taxa and enrich the diversity of Mucoromycota.

Analysis of Consumer Behavior in the Context of the Digital Economy

Under the condition that the digital economy model has penetrated into the consumption field,the consumer market environment is improving,the consumption structure is upgrading,the consumption content is diversified,and the consumer behavior is showing new epoch-making characteristics.But at the same time,there are also some problems,such as consumer privacy disclosure,and price discrimination based on consumer behavior.The future development of consumer economy needs the joint efforts of the government,the market and consumers to establish a new cycle of the economic system from top to bottom.

Improvement of Poplar Bark Extract on Physiological Characters of Lactococcus lactis in Intestine of White Feather Broilers

[Objective]The paper was to study the improvement of poplar bark extract on intestinal Lactococcus lactis of white feather broilers.[Method]Totally 450 Ross 308 white-feather broilers were randomly divided into five groups:control group,low dose group,medium dose group,high dose group,and antibiotic group(oxytetracycline hydrochloride).The feeding duration was 45 d.The probiotics were screened and isolated through homology,and the physiological and biochemical characteristics of chicken intestinal bacteria in different groups were compared to determine the properties of bacterial strain.The drug resistance,antibacterial ability,proliferation ability,acid resistance and bile salt resistance of isolated strain were tested,and a strain of L.lactis was obtained.[Result]The isolated L.lactis was sensitive to other drugs except natural tetracyclines,and there was no significant difference among the four groups except oxytetracycline group;as the concentration of extract increased,the inhibition of L.lactis against Salmonella sp.increased;the medium dose extract had the largest increase in the ability to tolerate the proliferation of L.lactis.[Conclusion]Feeding poplar bark extract will produce positive effects on the physiological characters of intestinal L.lactis in broiler chicken,which will provide potential probiotic strain for chicken production.

Study on Efficient Multiplication of Blueberry(Vaccinium corymbosum)Plantlets in Swinging type Temporary Immersion Bioreactors System(S-TIBs)

The swinging type temporary immersion bioreactors system （S-TTBs） is a kind of new and advanced method of tissue culture. The efficient multiplica- tion technology of blueberry （ Vaccinium corymbosum） plantlets in S-TTBs was systematically studied through L9 （3^4） orthogonal experiment with three factors （culture medimn volmne, swing angle, and inoculation density） and completely randomized experiment with two factors （swing frequency and immersion time）. The results showed that the optimal culture parameters were set as follows： the culture medium volume of 250 nil/bottle, the swing angle at 45 ° , and the inoculation density of 60 plantlets/bottle. The optimal swing frequency was 1 time/6 h, and the immersion time was set as 60 s.