Objectives Most medical instruments are designed for diagnosis purpose but very few for clinical treatment. Our research aim is to design and develop a cardiovascular automatic feedback control instrument （CAFCI） fo...Objectives Most medical instruments are designed for diagnosis purpose but very few for clinical treatment. Our research aim is to design and develop a cardiovascular automatic feedback control instrument （CAFCI） for rescuing the critical patients with abnormal blood pressure. Methods The CAFCI was designed on the basis of abundant clinical experiences and on successful mathematic modeling of our blood pressure, pulmonary experimental data. The capillary wedge pressure, and rates of heart beat were measured and inputted into a computer and drugs were chosen by a doctor through a user-friendly interface with the computer. The responses to medication were rapidly acquired and feed back to the computer by automatic detection system in a close-loop system. every 7.5 sec in order to dosage The data were refreshed regulate the speed and of the medications that were given. Results The experimental results with ten dogs showed that the CAFCI system took samples promptly and accurately so that the targeted blood pressure could be reached reliably based on our input parameters and our designing requirements. Conclusions Since the dependability and accuracy of the CAFCI system are much superior to that of the traditional method, its clinical application to rescue the critical patient warrants evaluation in the future.展开更多
Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to veri...Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to verify the effects of volatile oil of Nardostachy chinesis Batal （Gansong）. Methods Two pulses generated by computer followed by a recovery pulse and a test pulse, the interval duration between the two pulses varied from 16 ms to 1 s, and holding potential is -80 mV to - 140 inV. The peak Na^＋ current for a given recovery time was normalized to the tully recovered peak current, and the normalized value was the plot as a function of the recovery time to study the effects of 3 ppm concentration Gansong volatile oil on recovery from use-dependent block of Navl. 5 in HEK. Results It showed that Gansong group, comparing with control group, delayed the time courses of recovery from use-dependent block [ （33.2± 5.77 ） ms for control group and （52.5± 6.08 ） ms for 3 ppm Gansong group, P 〈 0.05 ] In the presence of Gansong, inhibition of the Na^＋ current was enhanced by increasing frequency of depolarizing pulse from 56.5 ms to 16 ms. In the control group, the time course of recovery showed that recovery started at 19.5 ms and finished by 36.5 ms. In the presence of Gansong, the time course of recovery showed that recovery started at 36.5 ms and finished by 56.5 ms. Na^＋ currents recovered from the use-dependent block varying with holding potential （holding potential-dependent）. Conclusions The results suggested that Na ＋ currents recovered from the use-dependent block correlated with persistent time, holding potential. The Gansong volatile oil has inhibitive effect on the Na^＋ current recovery.展开更多
文摘Objectives Most medical instruments are designed for diagnosis purpose but very few for clinical treatment. Our research aim is to design and develop a cardiovascular automatic feedback control instrument （CAFCI） for rescuing the critical patients with abnormal blood pressure. Methods The CAFCI was designed on the basis of abundant clinical experiences and on successful mathematic modeling of our blood pressure, pulmonary experimental data. The capillary wedge pressure, and rates of heart beat were measured and inputted into a computer and drugs were chosen by a doctor through a user-friendly interface with the computer. The responses to medication were rapidly acquired and feed back to the computer by automatic detection system in a close-loop system. every 7.5 sec in order to dosage The data were refreshed regulate the speed and of the medications that were given. Results The experimental results with ten dogs showed that the CAFCI system took samples promptly and accurately so that the targeted blood pressure could be reached reliably based on our input parameters and our designing requirements. Conclusions Since the dependability and accuracy of the CAFCI system are much superior to that of the traditional method, its clinical application to rescue the critical patient warrants evaluation in the future.
基金National Natural Science Foundation of People's Republic of China(No.30660060)
文摘Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use- dependent block of Na ＋ channels （Nav 1.5） in human embryonic kidney （HEK） cells, on which to verify the effects of volatile oil of Nardostachy chinesis Batal （Gansong）. Methods Two pulses generated by computer followed by a recovery pulse and a test pulse, the interval duration between the two pulses varied from 16 ms to 1 s, and holding potential is -80 mV to - 140 inV. The peak Na^＋ current for a given recovery time was normalized to the tully recovered peak current, and the normalized value was the plot as a function of the recovery time to study the effects of 3 ppm concentration Gansong volatile oil on recovery from use-dependent block of Navl. 5 in HEK. Results It showed that Gansong group, comparing with control group, delayed the time courses of recovery from use-dependent block [ （33.2± 5.77 ） ms for control group and （52.5± 6.08 ） ms for 3 ppm Gansong group, P 〈 0.05 ] In the presence of Gansong, inhibition of the Na^＋ current was enhanced by increasing frequency of depolarizing pulse from 56.5 ms to 16 ms. In the control group, the time course of recovery showed that recovery started at 19.5 ms and finished by 36.5 ms. In the presence of Gansong, the time course of recovery showed that recovery started at 36.5 ms and finished by 56.5 ms. Na^＋ currents recovered from the use-dependent block varying with holding potential （holding potential-dependent）. Conclusions The results suggested that Na ＋ currents recovered from the use-dependent block correlated with persistent time, holding potential. The Gansong volatile oil has inhibitive effect on the Na^＋ current recovery.
