Objective:To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV)IgM,IgG and other antibody indicators in mice.Methods:A total of 90 mice with HCV were...Objective:To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV)IgM,IgG and other antibody indicators in mice.Methods:A total of 90 mice with HCV were selected as the research objects.All the mice were tested for HCV IgM and IgA antibody,and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay,magnetic particle chemiluminescence method and indirect agglutination method,and the results and positive rates of the three groups were compared.Results:There was no significant difference in the positive rate among the three groups(P>0.05).The specificity,accuracy and sensitivity of indirect agglutination test for IgM were 90.79%,80.00%and 30.00%,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44%,93.33%and80.00%,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P<0.05).For IgA,the specificity,accuracy and sensitivity of indirect agglutination assay were 90.67%,78.66%and 20.00%,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25%,92.35%and80.00%,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay,and the difference was statistically significant(P<0.05).Conclusion:The magnetic particle chemiluminescence method is more effective in the detection of IgM,IgG and other antibodies in mice with HCV.展开更多
Osteoarthritis is a common clinical chronic degenerative disease with a gradually increasing morbidity as the population ages.The pathogenesis of the disease always maintained as a focus of clinicians’research.At pre...Osteoarthritis is a common clinical chronic degenerative disease with a gradually increasing morbidity as the population ages.The pathogenesis of the disease always maintained as a focus of clinicians’research.At present,the etiology and pathogenesis of osteoarthritis are far from clear.The attack of the disease has something to do with the patient’s joint inflammation,trauma and genetic factors,which is a result of joint action of multiple factors.Trace elements are elements indispensable in the growth and development of the body.Despite it stiny content in the human body,it produces powerful biological effects and plays an important role in the growth and metabolism of the body.This paper studies the relevance between trace elements and osteoarthritis.展开更多
Infectious diseases feature multiple pathogens,complicated and diverse clinical symptoms.Early and accurate detection of infectious pathogens sets the foundation for its targeted clinical treatment.With long detection...Infectious diseases feature multiple pathogens,complicated and diverse clinical symptoms.Early and accurate detection of infectious pathogens sets the foundation for its targeted clinical treatment.With long detection cycles and low positive rates,traditional detection approaches such as Gram-stained smear microscopy and specific marker detection can barely meet the clinical needs for infectious disease detection.As a new gene detection technology,macrogeneⅡsequencing can enable higher detection efficiency for infectious diseases caused by pathogens such as fungi and bacteria,demonstrating certain application value.This paper describes invasive pulmonary aspergillosis(IPA),concepts concerning macrogeneⅡsequencing,as well as its application value for invasive pulmonary aspergillosis.展开更多
The prognosis of small cell lung cancer is poor.Almost all patients have a tendency for tumor spreading to the whole body when they are diagnosed.Therefore,the combination of radiotherapy and chemotherapy is the main ...The prognosis of small cell lung cancer is poor.Almost all patients have a tendency for tumor spreading to the whole body when they are diagnosed.Therefore,the combination of radiotherapy and chemotherapy is the main treatment,and surgical treatment is only implemented in a small number of limited patients.In recent years,there has been no obvious breakthrough in the treatment of small cell lung cancer,and the overall poor prognosis has not changed.With the development of medical technology,immune checkpoint inhibitors are gradually applied to the treatment of small-cell lung cancer,but the overall effect is limited.How to screen the beneficiary population to further improve the effect of immunotherapy is the key issue in the treatment of small-cell lung cancer.展开更多
文摘Objective:To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV)IgM,IgG and other antibody indicators in mice.Methods:A total of 90 mice with HCV were selected as the research objects.All the mice were tested for HCV IgM and IgA antibody,and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay,magnetic particle chemiluminescence method and indirect agglutination method,and the results and positive rates of the three groups were compared.Results:There was no significant difference in the positive rate among the three groups(P>0.05).The specificity,accuracy and sensitivity of indirect agglutination test for IgM were 90.79%,80.00%and 30.00%,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44%,93.33%and80.00%,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P<0.05).For IgA,the specificity,accuracy and sensitivity of indirect agglutination assay were 90.67%,78.66%and 20.00%,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25%,92.35%and80.00%,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay,and the difference was statistically significant(P<0.05).Conclusion:The magnetic particle chemiluminescence method is more effective in the detection of IgM,IgG and other antibodies in mice with HCV.
文摘Osteoarthritis is a common clinical chronic degenerative disease with a gradually increasing morbidity as the population ages.The pathogenesis of the disease always maintained as a focus of clinicians’research.At present,the etiology and pathogenesis of osteoarthritis are far from clear.The attack of the disease has something to do with the patient’s joint inflammation,trauma and genetic factors,which is a result of joint action of multiple factors.Trace elements are elements indispensable in the growth and development of the body.Despite it stiny content in the human body,it produces powerful biological effects and plays an important role in the growth and metabolism of the body.This paper studies the relevance between trace elements and osteoarthritis.
文摘Infectious diseases feature multiple pathogens,complicated and diverse clinical symptoms.Early and accurate detection of infectious pathogens sets the foundation for its targeted clinical treatment.With long detection cycles and low positive rates,traditional detection approaches such as Gram-stained smear microscopy and specific marker detection can barely meet the clinical needs for infectious disease detection.As a new gene detection technology,macrogeneⅡsequencing can enable higher detection efficiency for infectious diseases caused by pathogens such as fungi and bacteria,demonstrating certain application value.This paper describes invasive pulmonary aspergillosis(IPA),concepts concerning macrogeneⅡsequencing,as well as its application value for invasive pulmonary aspergillosis.
文摘The prognosis of small cell lung cancer is poor.Almost all patients have a tendency for tumor spreading to the whole body when they are diagnosed.Therefore,the combination of radiotherapy and chemotherapy is the main treatment,and surgical treatment is only implemented in a small number of limited patients.In recent years,there has been no obvious breakthrough in the treatment of small cell lung cancer,and the overall poor prognosis has not changed.With the development of medical technology,immune checkpoint inhibitors are gradually applied to the treatment of small-cell lung cancer,but the overall effect is limited.How to screen the beneficiary population to further improve the effect of immunotherapy is the key issue in the treatment of small-cell lung cancer.
