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Ultrasensitive assay of ctDNA based on DNA triangular prism and three-way junction nanostructures
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作者 Hua Chai Peng Miao 《Chinese Chemical Letters》 SCIE CAS CSCD 2021年第2期783-786,共4页
Circulating tumor DNA(ctDNA) refers to a class of acellular nucleic acids carrying genetic features of primary tumor,which can be regarded as a promising noninvasive biomarker for cancer diagnosis.The development of c... Circulating tumor DNA(ctDNA) refers to a class of acellular nucleic acids carrying genetic features of primary tumor,which can be regarded as a promising noninvasive biomarker for cancer diagnosis.The development of ctDNA assay is an important component of liquid biopsy.In this study,we have fabricated a novel electrochemical strategy for ultrasensitive detection of ctDNA combining the merits of strand displacement amplification and DNA nanostructures.Stable DNA triangular prism is firstly selfassembled and modified on the electrode surface.After target initiated strand displacement polymerization reaction,the generated DNA product helps the formation of three-way junction nanostructure on triangular prism,which localizes electrochemical species.By carefully investigating the electrochemical responses,the limit of detection(LOD) for ctDNA assay as low as 48 amol/L is achieved.This proposed electrochemical biosensor shows great potential for clinical applications. 展开更多
关键词 Circulating tumor DNA BIOSENSOR DNA triangular prism DNA three-way junction Strand displacement polymerization
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