Recent population studies have significantly advanced our understanding of how age shapes the gut microbiota.However,the actual role of age could be inevitably confounded due to the complex and variable environmental ...Recent population studies have significantly advanced our understanding of how age shapes the gut microbiota.However,the actual role of age could be inevitably confounded due to the complex and variable environmental factors in human populations.A well-controlled environment is thus necessary to reduce undesirable confounding effects,and recapitulate age-dependent changes in the gut microbiota of healthy primates.Herein we performed 16S rRNA gene sequencing,characterized the age-associated gut microbial profiles from infant to elderly crab-eating macaques reared in captivity,and systemically revealed the lifelong dynamic changes of the primate gut microbiota.While the most significant age-associated taxa were mainly found as commensals such as Faecalibacterium,the abundance of a group of suspicious pathogens such as Helicobacter was exclusively increased in infants,underlining their potential role in host development.Importantly,topology analysis indicated that the network connectivity of gut microbiota was even more agedependent than taxonomic diversity,and its tremendous decline with age could probably be linked to healthy aging.Moreover,we identified key driver microbes responsible for such age-dependent network changes,which were further linked to altered metabolic functions of lipids,carbohydrates,and amino acids,as well as phenotypes in the microbial community.The current study thus demonstrates the lifelong age-dependent changes and their driver microbes in the primate gut microbiota,and provides new insights into their roles in the development and healthy aging of their hosts.展开更多
基金supported in part by research grants from the Guangdong Academy of Sciences Special Project of Science and Technology Development,China(Grant No.2019GDASYL-0302007)the National Natural Science Foundation of China(Grant Nos.31671311 and 81170853)+11 种基金the Guangdong Science&Technology Project,China(Grant Nos.2017A070702014 and 2014B070706020)the National Key R&D Program of China(Grant No.2018YFA0901700)the National first-class discipline program of Light Industry Technology and Engineering,China(Grant No.LITE2018-14)the“Six Talent Peak”Plan of Jiangsu Province,China(Grant No.SWYY-127)the Guangdong Basic and Applied Basic Research Foundation,China(Grant No.2019A1515012062)the Guangdong Academy of Sciences Special Project of Science and Technology Development,China(Grant Nos.2018GDASCX-0107 and 2017GDASCX-0107)the Fundamental Research Funds for the Central Universities,China(Grant Nos.JUSRP51712B and JUSRP1901XNC)the Taihu Lake Talent Planthe Program for High-Level Entrepreneurial and Innovative Talents Introduction of Jiangsu Provincethe Guangdong High-level Personnel of Special Support Programthe Yangfan Plan of Talents Recruitment Grantthe Wuxi Institute of Translational Medicine,China.
文摘Recent population studies have significantly advanced our understanding of how age shapes the gut microbiota.However,the actual role of age could be inevitably confounded due to the complex and variable environmental factors in human populations.A well-controlled environment is thus necessary to reduce undesirable confounding effects,and recapitulate age-dependent changes in the gut microbiota of healthy primates.Herein we performed 16S rRNA gene sequencing,characterized the age-associated gut microbial profiles from infant to elderly crab-eating macaques reared in captivity,and systemically revealed the lifelong dynamic changes of the primate gut microbiota.While the most significant age-associated taxa were mainly found as commensals such as Faecalibacterium,the abundance of a group of suspicious pathogens such as Helicobacter was exclusively increased in infants,underlining their potential role in host development.Importantly,topology analysis indicated that the network connectivity of gut microbiota was even more agedependent than taxonomic diversity,and its tremendous decline with age could probably be linked to healthy aging.Moreover,we identified key driver microbes responsible for such age-dependent network changes,which were further linked to altered metabolic functions of lipids,carbohydrates,and amino acids,as well as phenotypes in the microbial community.The current study thus demonstrates the lifelong age-dependent changes and their driver microbes in the primate gut microbiota,and provides new insights into their roles in the development and healthy aging of their hosts.
