[ Objectives ] To analyze the genetic structure and genetic diversity of gold grass carp population. [ Methods ] A total of 15 microsatellite markers were used to examine gold grass carp population and four grass carp...[ Objectives ] To analyze the genetic structure and genetic diversity of gold grass carp population. [ Methods ] A total of 15 microsatellite markers were used to examine gold grass carp population and four grass carp populations[ Yuanjiang (YJ), Ningxiang (NX) , Honghu (HH) and Xijiang (XJ) ] from the Yangtze River and the Pearl River of China. [ Results] All the 15 microsatellite loei were highly polymorphic, with polymorphism information content (PIC) of 0.763 -0.939. The expected heterozygosity (HE ) of gold grass carp population was 0. 662, lower than those of the four Chinese grass carp populations (HE = 0.85 - 0. 885 ). The genetic differentiation index ( FST ) between gold grass carp population and YJ, NX, HH, XJ populations were 0. 157 2, 0. 129 5, 0. 147 5 and 0. 114 4, respectively. This suggests that gold grass carp population is highly differentiated from YJ grass carp population (0.15 〈 FST 〈0.25), while moderately differentiated from other three grass carp populations (0.05 〈 FST 〈 0.15 ). Gold grass carp population had the closest genetic relationship with XJ population (DA = 0.476 3), and it showed the farthest genetic relationship with YJ grass carp population (DA = 0. 810 7). The NJ phylogenetic tree based on genetic distance indicated that four Chinese grass carp populations clustered together as one branch, while gold grass carp population as another. [ Conclusions] Genetic di- versity of gold grass carp population is lower than that of Chinese grass carp population, with relative distant genetic relationship.展开更多
基金Supported by National Freshwater Fish Industry Technology System Project(CARS-46-03)Special Fund for Guangdong Provincial Marine Fishery Science and Technology and Industrial Development(A201401A03)Natural Science Foundation of Guangdong Province(2016A030313148)
文摘[ Objectives ] To analyze the genetic structure and genetic diversity of gold grass carp population. [ Methods ] A total of 15 microsatellite markers were used to examine gold grass carp population and four grass carp populations[ Yuanjiang (YJ), Ningxiang (NX) , Honghu (HH) and Xijiang (XJ) ] from the Yangtze River and the Pearl River of China. [ Results] All the 15 microsatellite loei were highly polymorphic, with polymorphism information content (PIC) of 0.763 -0.939. The expected heterozygosity (HE ) of gold grass carp population was 0. 662, lower than those of the four Chinese grass carp populations (HE = 0.85 - 0. 885 ). The genetic differentiation index ( FST ) between gold grass carp population and YJ, NX, HH, XJ populations were 0. 157 2, 0. 129 5, 0. 147 5 and 0. 114 4, respectively. This suggests that gold grass carp population is highly differentiated from YJ grass carp population (0.15 〈 FST 〈0.25), while moderately differentiated from other three grass carp populations (0.05 〈 FST 〈 0.15 ). Gold grass carp population had the closest genetic relationship with XJ population (DA = 0.476 3), and it showed the farthest genetic relationship with YJ grass carp population (DA = 0. 810 7). The NJ phylogenetic tree based on genetic distance indicated that four Chinese grass carp populations clustered together as one branch, while gold grass carp population as another. [ Conclusions] Genetic di- versity of gold grass carp population is lower than that of Chinese grass carp population, with relative distant genetic relationship.