Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify se...Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify sex-related genes.We recorded 281599294 bp of clean reads,and de novo assembly generated 85206 unigenes,with N_(50) of 1311 bp and average length of 763 bp.We found 5002 genes highly expressed in males and 2179 genes highly expressed in females.Expression profiles of six unigenes specific to females,six unigenes specific to males,and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR;results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis.Using all unigenes as references,we identified 6657 putative simple sequence repeats.The transcriptome sequences and gene expression profiles of female and male H.vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.展开更多
Immiscible displacements in porous media are fundamentally significant for many natural processes and industrial applications.Although extensive work has been done in this field,some limitations still exist due to the...Immiscible displacements in porous media are fundamentally significant for many natural processes and industrial applications.Although extensive work has been done in this field,some limitations still exist due to the difficulty to cover the large number of influencing factors.In this paper,we present a systematic study involving the coupled influence of capillary forces,viscous forces,wetting properties and pore geometries.By microfluidic experiments with high resolutions,both residual fluid distributions and dynamical invasion processes were clearly captured and quantitatively characterized.A void-filling behavior was identified for drainage as the capillary number Ca increased.An anomalous void-leaving behavior was found for the case of imbibition with an unfavorable viscosity ratio M,representing many ribbon-like regions invaded at intermediate Ca turned to be not accessible at high Ca.A pore geometry with a large typical pore-throat ratio seemed to enhance the void-leaving behavior.During the dynamical invasion processes,an intermittency behavior was captured in the form of a fluctuation of the increasing rate of the invading saturation with time.The intermittency dynamics were most evident for drainage with an unfavorable M.A pore geometry with high porosity acted to suppress the intermittency behaviors.Our experimental results sketched out a full view of immiscible displacements in porous media under different conditions,and provided a complete dataset which could be used to test the rapid developing pore-scale models.展开更多
基金the National Key R and DProgram of China(Grant No.2017YFD0200900)the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2017).
文摘Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify sex-related genes.We recorded 281599294 bp of clean reads,and de novo assembly generated 85206 unigenes,with N_(50) of 1311 bp and average length of 763 bp.We found 5002 genes highly expressed in males and 2179 genes highly expressed in females.Expression profiles of six unigenes specific to females,six unigenes specific to males,and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR;results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis.Using all unigenes as references,we identified 6657 putative simple sequence repeats.The transcriptome sequences and gene expression profiles of female and male H.vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.
基金supported by the National Natural Science Foundation of China(51876100)the Science Fund for Creative Research Group(No.51621062).
文摘Immiscible displacements in porous media are fundamentally significant for many natural processes and industrial applications.Although extensive work has been done in this field,some limitations still exist due to the difficulty to cover the large number of influencing factors.In this paper,we present a systematic study involving the coupled influence of capillary forces,viscous forces,wetting properties and pore geometries.By microfluidic experiments with high resolutions,both residual fluid distributions and dynamical invasion processes were clearly captured and quantitatively characterized.A void-filling behavior was identified for drainage as the capillary number Ca increased.An anomalous void-leaving behavior was found for the case of imbibition with an unfavorable viscosity ratio M,representing many ribbon-like regions invaded at intermediate Ca turned to be not accessible at high Ca.A pore geometry with a large typical pore-throat ratio seemed to enhance the void-leaving behavior.During the dynamical invasion processes,an intermittency behavior was captured in the form of a fluctuation of the increasing rate of the invading saturation with time.The intermittency dynamics were most evident for drainage with an unfavorable M.A pore geometry with high porosity acted to suppress the intermittency behaviors.Our experimental results sketched out a full view of immiscible displacements in porous media under different conditions,and provided a complete dataset which could be used to test the rapid developing pore-scale models.
