Bulked segregant analysis(BSA)is an efficient and low-cost strategy that is widely used to identify causal genes in segregating populations.BSA-based methods,such as BSA sequencing(Wenger et al.,2010),bulked segregant...Bulked segregant analysis(BSA)is an efficient and low-cost strategy that is widely used to identify causal genes in segregating populations.BSA-based methods,such as BSA sequencing(Wenger et al.,2010),bulked segregant RNA sequencing(BSR-seq)(del Viso et al.,2012),and MutMap(Abe et al.,2012),are powerful tools that can be used for rapidly discovering genetic markers and gene mapping.Although BSA is increasingly being used in wheat(Triticum aestivum)gene mapping efforts,few user-friendly BSA tools have been developed for researchers lacking a strong bioinformatics background.Here,we developed the web-based BSA platform WheatGmap(https://www.wheatgmap.org),which integrates multiple BSA mapping models and large amounts of public data to accelerate gene cloning and functional research and facilitate resource sharing.展开更多
基金This work was supported by the National Key Research and Development Program of China(2016YFD0101802,2018YFE0112000,2016YFD0101001,and 2017YFD0100900)the National Transgenic Research Project of China(2016ZX08009001-001-004)+2 种基金the Talent Program and Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS)the Tianshan Innovation Team Plan(2020D14002)the National Natural Science Foundation of China(31871609,91935303 and 31771792).
文摘Bulked segregant analysis(BSA)is an efficient and low-cost strategy that is widely used to identify causal genes in segregating populations.BSA-based methods,such as BSA sequencing(Wenger et al.,2010),bulked segregant RNA sequencing(BSR-seq)(del Viso et al.,2012),and MutMap(Abe et al.,2012),are powerful tools that can be used for rapidly discovering genetic markers and gene mapping.Although BSA is increasingly being used in wheat(Triticum aestivum)gene mapping efforts,few user-friendly BSA tools have been developed for researchers lacking a strong bioinformatics background.Here,we developed the web-based BSA platform WheatGmap(https://www.wheatgmap.org),which integrates multiple BSA mapping models and large amounts of public data to accelerate gene cloning and functional research and facilitate resource sharing.
