Biofunctional europium（Ⅲ）-doped ZnS（ZnS：Eu） nanocrystals are prepared by a sol–gel method. The characteristic luminescence of ZnS：Eu is used as a probe signal to realize sensitive immunoassay. The luminescence...Biofunctional europium（Ⅲ）-doped ZnS（ZnS：Eu） nanocrystals are prepared by a sol–gel method. The characteristic luminescence of ZnS：Eu is used as a probe signal to realize sensitive immunoassay. The luminescence intensity of the Eu^（3＋） in the ZnS matrix shows strong concentration dependence, and the optimal doping concentration is 4%. However,the emission wavelengths of the ZnS：Eu nanocrystals are not dependent on doping concentration nor the temperature（from 100 K to 300 K）. Our results show that these features allow for reliable immunoassay. Human immunoglobulin, used as a target analyte, is captured by antibody modified ZnS：Eu probe and is finally enriched on gold substrate for detection.High specificity of the assay is demonstrated by control experiments. The linear detection range is 10 nM –800 nM, and the detection limit is about 9.6 nM.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant No.61205193)the Project of Science and Technology of Jilin Province,China(Grant No.20140520107JH)+1 种基金the Technology Foundation of Jilin Provincial Department of Human Resources and Social Security,China(Grant No.RL201306)the Science Foundation for Young Scientists of Changchun University of Science and Technology,China(Grant No.XQNJJ-2015-03)
文摘Biofunctional europium（Ⅲ）-doped ZnS（ZnS：Eu） nanocrystals are prepared by a sol–gel method. The characteristic luminescence of ZnS：Eu is used as a probe signal to realize sensitive immunoassay. The luminescence intensity of the Eu^（3＋） in the ZnS matrix shows strong concentration dependence, and the optimal doping concentration is 4%. However,the emission wavelengths of the ZnS：Eu nanocrystals are not dependent on doping concentration nor the temperature（from 100 K to 300 K）. Our results show that these features allow for reliable immunoassay. Human immunoglobulin, used as a target analyte, is captured by antibody modified ZnS：Eu probe and is finally enriched on gold substrate for detection.High specificity of the assay is demonstrated by control experiments. The linear detection range is 10 nM –800 nM, and the detection limit is about 9.6 nM.
基金supported by the National Natural Science Foundation of China (51502146, U1404506, 21671113, 51772305, 51572270, and U1662118)the International Partnership Program of Chinese Academy of Sciences (GJHZ1819)+1 种基金the Royal Society-Newton Advanced Fellowship (NA170422)supported by Open Fund (PEBM201702) of Key Laboratory for Photonic and Electric Bandgap Materials, Ministry of Education (Harbin Normal University)
