Objective： To observe the effect of electroacupuncture（EA） at Zusanli（ST 36） on the gastrointestinal motility and the ultrastructures of pacemaker cells [the interstitial cells of Cajal（ICC）] in diabetic gastro...Objective： To observe the effect of electroacupuncture（EA） at Zusanli（ST 36） on the gastrointestinal motility and the ultrastructures of pacemaker cells [the interstitial cells of Cajal（ICC）] in diabetic gastroparesis（DGP） rats and explore the mechanism of EA for DGP.Methods： A total of 50 Sprague-Dawley（SD） rats were randomly divided into group A, group B, group C, group D and group E, with 10 rats in each group. Group A was the blank control; a single intraperitoneal injection of 2% streptozotocin（STZ） was performed in rats of group B, group C, group D and group E, with high glucose and high fat diet for 8 weeks to establish the DGP rat models. Group B was the model group and the rats did not receive any treatment; group C was EA at acupoint group and the rats received EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）; group D was EA at non-acupoint group and the rats received EA at the control points of Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）; group E was metoclopramide group and the rats were treated by intragastric administration of metoclopramide. Blood glucose was detected using ONE TOUCH blood glucose meter; gastric emptying rate and small intestine migration rate were measured using intragastric phenol red; ultrastructures of gastric antrum ICC were detected by transmission electron microscopy. Results： The differences of blood glucose between group B, group C, group D, group E versus that of group A were statistically significant after modeling（P〈0.01）; after treatment, the differences of blood glucose between group D, group E versus that of group C were statistically significant（P〈0.05, P〈0.01）; the gastric emptying rate of rats in group B was statistically significant different from that in group A（P〈0.01）; the gastric emptying rate of rats in group C was statistically significant different from that in group B（P〈0.01）. The migration rates of rats＇ small intestines in group B, group C, group D and group E were all statistically significant different from that in group A（P〈0.01）; the migration rate of rats＇ small intestines in group C was statistically significant different from that in group B（P〈0.01）. The ultrastructure of rat＇s ICC in group B showed apoptosis compared with that in group A; rat‘s ICC in group C had complete basement membrane, more cytoplasm mitochondria, Golgi and rough endoplasmic reticulum, showing clear structure, occasional mitochondria swelling and gap junctions with adjacent smooth muscle cells; there were no significant differences between group D, group E versus group B. Conclusion： EA at Zusanli（ST 36） plus other acupoints can regulate the blood glucose and promote gastrointestinal motility in DGP rats, and the mechanism may be related to repairing the damaged ICC structure.展开更多
基金supported by National Natural Science Foundation of China,No.81403487Youth Fund of Hunan Province Education Office,No.14B128~~
文摘Objective： To observe the effect of electroacupuncture（EA） at Zusanli（ST 36） on the gastrointestinal motility and the ultrastructures of pacemaker cells [the interstitial cells of Cajal（ICC）] in diabetic gastroparesis（DGP） rats and explore the mechanism of EA for DGP.Methods： A total of 50 Sprague-Dawley（SD） rats were randomly divided into group A, group B, group C, group D and group E, with 10 rats in each group. Group A was the blank control; a single intraperitoneal injection of 2% streptozotocin（STZ） was performed in rats of group B, group C, group D and group E, with high glucose and high fat diet for 8 weeks to establish the DGP rat models. Group B was the model group and the rats did not receive any treatment; group C was EA at acupoint group and the rats received EA at Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）; group D was EA at non-acupoint group and the rats received EA at the control points of Zusanli（ST 36）, Liangmen（ST 21） and Sanyinjiao（SP 6）; group E was metoclopramide group and the rats were treated by intragastric administration of metoclopramide. Blood glucose was detected using ONE TOUCH blood glucose meter; gastric emptying rate and small intestine migration rate were measured using intragastric phenol red; ultrastructures of gastric antrum ICC were detected by transmission electron microscopy. Results： The differences of blood glucose between group B, group C, group D, group E versus that of group A were statistically significant after modeling（P〈0.01）; after treatment, the differences of blood glucose between group D, group E versus that of group C were statistically significant（P〈0.05, P〈0.01）; the gastric emptying rate of rats in group B was statistically significant different from that in group A（P〈0.01）; the gastric emptying rate of rats in group C was statistically significant different from that in group B（P〈0.01）. The migration rates of rats＇ small intestines in group B, group C, group D and group E were all statistically significant different from that in group A（P〈0.01）; the migration rate of rats＇ small intestines in group C was statistically significant different from that in group B（P〈0.01）. The ultrastructure of rat＇s ICC in group B showed apoptosis compared with that in group A; rat‘s ICC in group C had complete basement membrane, more cytoplasm mitochondria, Golgi and rough endoplasmic reticulum, showing clear structure, occasional mitochondria swelling and gap junctions with adjacent smooth muscle cells; there were no significant differences between group D, group E versus group B. Conclusion： EA at Zusanli（ST 36） plus other acupoints can regulate the blood glucose and promote gastrointestinal motility in DGP rats, and the mechanism may be related to repairing the damaged ICC structure.
