Not from concentrate(NFC)fruit juice is the crucial clean label ingredient for new-style tea-making due to its pleasant color and fresh aroma.Here,we compared the effects of mild heating(MH)and high pressure processin...Not from concentrate(NFC)fruit juice is the crucial clean label ingredient for new-style tea-making due to its pleasant color and fresh aroma.Here,we compared the effects of mild heating(MH)and high pressure processing(HPP)on physicochemical characters and phytochemicals in NFC spine grape juice based on metabolomics analysis.Similar compound profiles were observed between HPP-treated and fresh juices.The richer phytochemical compounds comprised malvidin-3-O-glucoside,malvidin-3,5-di-O-glucoside,quercetin-3-O-rhamnoside,quercetin-3-Oglucuronide,catechin,caffeic acid,ferulic acid,procyanidin B1,procyanidin B2 were obtained after MH treatment.Nine marker phenolics and two marker tripeptides(i.e.,Glu-Val-Phe and Leu-Leu-Tyr)were identified to differentiate MH from HPP treatment,of which higher contents occurred in the MH group.Storage time experiments showed that the Glu-Val-Phe could serve as potential markers for monitoring storage of spine grape juice.These results provide new insights into the effects of processing on individual phytochemical changes and the guide for commercial application of production of spine grape NFC juice.展开更多
A simple, effective and reliable synthetic method was developed to construct beta-agonist phenylethano- lamine A（PEAA） in good yield with 4-nitrobenzyl bromide as the starting material, which underwent SN2 reaction ...A simple, effective and reliable synthetic method was developed to construct beta-agonist phenylethano- lamine A（PEAA） in good yield with 4-nitrobenzyl bromide as the starting material, which underwent SN2 reaction with ethyl acetoacetate, followed by hydrolysis with concentrated HC1 and amination by Lettkart reaction to furnish 4-（4-nitrophenyl）butan-2-amine（A）. A one-pot reaction was studied to construct PEAA skeleton and reduced carbonyl with KBH4, the conditions were optimized, including reaction temperature, reaction time, solvent and the molar ratio of 2-bromo-l-（4-methoxyphenyl）ethanone（B） to A. Meanwhile, a reliable and practical high performance liquid chromography（HPLC） method with UV detector was developed after screening wavelength, chromatographic column and optimization of mobile phase. The optimal conditions of detecting PEAA were water（0.1% formic acid and 0.1% triethylamine）-methanol（volume ratio 44：56） at 278 nm with plus-C^8 column. The above synthetic method would be used to synthesize standard reference materials and advance extensive application of analytical method. In order to ensure food safety and safeguard human health, the analytical method might be used as a standard method in detection and supervision of PEAA.展开更多
基金The authors acknowledge financial support for this work from National Key R&D Program of China(2022YFD2100805)National Science Fund for Excellent Young Scholars of China(32222067).
文摘Not from concentrate(NFC)fruit juice is the crucial clean label ingredient for new-style tea-making due to its pleasant color and fresh aroma.Here,we compared the effects of mild heating(MH)and high pressure processing(HPP)on physicochemical characters and phytochemicals in NFC spine grape juice based on metabolomics analysis.Similar compound profiles were observed between HPP-treated and fresh juices.The richer phytochemical compounds comprised malvidin-3-O-glucoside,malvidin-3,5-di-O-glucoside,quercetin-3-O-rhamnoside,quercetin-3-Oglucuronide,catechin,caffeic acid,ferulic acid,procyanidin B1,procyanidin B2 were obtained after MH treatment.Nine marker phenolics and two marker tripeptides(i.e.,Glu-Val-Phe and Leu-Leu-Tyr)were identified to differentiate MH from HPP treatment,of which higher contents occurred in the MH group.Storage time experiments showed that the Glu-Val-Phe could serve as potential markers for monitoring storage of spine grape juice.These results provide new insights into the effects of processing on individual phytochemical changes and the guide for commercial application of production of spine grape NFC juice.
文摘A simple, effective and reliable synthetic method was developed to construct beta-agonist phenylethano- lamine A（PEAA） in good yield with 4-nitrobenzyl bromide as the starting material, which underwent SN2 reaction with ethyl acetoacetate, followed by hydrolysis with concentrated HC1 and amination by Lettkart reaction to furnish 4-（4-nitrophenyl）butan-2-amine（A）. A one-pot reaction was studied to construct PEAA skeleton and reduced carbonyl with KBH4, the conditions were optimized, including reaction temperature, reaction time, solvent and the molar ratio of 2-bromo-l-（4-methoxyphenyl）ethanone（B） to A. Meanwhile, a reliable and practical high performance liquid chromography（HPLC） method with UV detector was developed after screening wavelength, chromatographic column and optimization of mobile phase. The optimal conditions of detecting PEAA were water（0.1% formic acid and 0.1% triethylamine）-methanol（volume ratio 44：56） at 278 nm with plus-C^8 column. The above synthetic method would be used to synthesize standard reference materials and advance extensive application of analytical method. In order to ensure food safety and safeguard human health, the analytical method might be used as a standard method in detection and supervision of PEAA.
