The means of survival of genomic dsRNA of reoviruses from dsRNA-triggered and Dicer-initiated RNAi pathway remains to be defined. The present study aimed to investigate the effect of Grass carp reovirus (GCRV) replica...The means of survival of genomic dsRNA of reoviruses from dsRNA-triggered and Dicer-initiated RNAi pathway remains to be defined. The present study aimed to investigate the effect of Grass carp reovirus (GCRV) replication on the RNAi pathway of grass carp kidney cells (CIK). The dsRNA-triggered RNAi pathway was demonstrated unimpaired in CIK cells through RNAi assay. GCRV-specific siRNA was generated in CIK cells transfected with purified GCRV genomic dsRNA in Northern blot analysis; while in GCRV-infected CIK cells, no GCRV-specific siRNA could be detected. Infection and transfection experiments further indicated that replication of GCRV correlated with the increased transcription level of the Dicer gene and functional inhibition of in vitro synthesized egfp-siRNA in silencing the EGFP reporter gene. These data demonstrated that although only the genomic dsRNA of GCRV was sensitive to the cellular RNAi pathway, unidentified RNAi suppressor protein(s) might contribute to the survival of the viral genome and efficient viral replication.展开更多
The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-...The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-related quantitative traits in this fish are limited.Heritability,the most important parameter in selective breeding programs,was extensively studied for the growth-related traits.The values varied widely among the experiments and methods used because of the existence of common environmental,dominance and maternal effects.However,correlations in phenotypic and genetic levels first evaluated several years ago were limited.On the other hand,heterosis was widely reported and easily obtained for growth-related traits in the common carp.Meanwhile,genotype environment interaction and prediction of breeding values have been studied recently,and are very important in conducting selective breeding programs.The developmental quantitative genetics of growth-related traits was first analyzed in the common carp for reasonable selection during ontogeny.It is expected that genetic improvement will be achieved by carrying out direct selective breeding in the common carp.展开更多
基金The Shanghai committee of Science and Technology(Grant No.10PJ1404800)the National Natural Science Foundation of China(Grant No.31072244)
文摘The means of survival of genomic dsRNA of reoviruses from dsRNA-triggered and Dicer-initiated RNAi pathway remains to be defined. The present study aimed to investigate the effect of Grass carp reovirus (GCRV) replication on the RNAi pathway of grass carp kidney cells (CIK). The dsRNA-triggered RNAi pathway was demonstrated unimpaired in CIK cells through RNAi assay. GCRV-specific siRNA was generated in CIK cells transfected with purified GCRV genomic dsRNA in Northern blot analysis; while in GCRV-infected CIK cells, no GCRV-specific siRNA could be detected. Infection and transfection experiments further indicated that replication of GCRV correlated with the increased transcription level of the Dicer gene and functional inhibition of in vitro synthesized egfp-siRNA in silencing the EGFP reporter gene. These data demonstrated that although only the genomic dsRNA of GCRV was sensitive to the cellular RNAi pathway, unidentified RNAi suppressor protein(s) might contribute to the survival of the viral genome and efficient viral replication.
基金supported by the Natural Science Foundation of China(Grant No.30700622)the Shanghai Education Committee Project(No.07ZZ136)the Shanghai Leading Academic Discipline Project(No.Y1101).
文摘The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-related quantitative traits in this fish are limited.Heritability,the most important parameter in selective breeding programs,was extensively studied for the growth-related traits.The values varied widely among the experiments and methods used because of the existence of common environmental,dominance and maternal effects.However,correlations in phenotypic and genetic levels first evaluated several years ago were limited.On the other hand,heterosis was widely reported and easily obtained for growth-related traits in the common carp.Meanwhile,genotype environment interaction and prediction of breeding values have been studied recently,and are very important in conducting selective breeding programs.The developmental quantitative genetics of growth-related traits was first analyzed in the common carp for reasonable selection during ontogeny.It is expected that genetic improvement will be achieved by carrying out direct selective breeding in the common carp.
