[Objectives]To investigate the effect of low temperature treatment on cotton leaves at the two-leaf stage.[Methods]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They w...[Objectives]To investigate the effect of low temperature treatment on cotton leaves at the two-leaf stage.[Methods]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Results]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Conclusions]These genes may play an important role in improving the cold resistance of cotton.展开更多
Background： With the rapid development of genomics, many functional genes have been targeted. Molecular marker assisted selection can accelerate the breeding process by linking selection to functional genes. Methods...Background： With the rapid development of genomics, many functional genes have been targeted. Molecular marker assisted selection can accelerate the breeding process by linking selection to functional genes. Methods： In a study of upland cotton （Gossypium hirsutum L.）, the F2 segregated population was constructed by crossing X1570 （short branches） with Ekangmian 13 （long branches） to identify the short fruiting branch gene and marker assisted selection with SNP（Single Nucleotide Polymorphisms, SNP） linked to its trait. Result： The result demonstrated that linked SSR marker BNL3232 was screened by BSA（Bulked segregant analysis, BSA） method; one SNP locus was found, which was totally separated from the fruiting branches trait in upland cotton. Conclusion： It was verified that this SNP marker could be used for molecular assisted selection of cotton architecture展开更多
基金Supported by Integration and Demonstration Project of Simple and Efficient Cultivation Technology of Cotton(2020YFD1001000)Special Fund for Construction of Modern Agricultural Industrial Technology SystemProject of Hubei Agricultural Science and Technology Innovation Center(2019-620-000-06)
文摘[Objectives]To investigate the effect of low temperature treatment on cotton leaves at the two-leaf stage.[Methods]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Results]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Conclusions]These genes may play an important role in improving the cold resistance of cotton.
基金Sponsored by State Key Laboratory of Cotton Biology Open Fund(CB2016A07)Hubei Provincial Agricultural Science and Technology Innovation Center Support Project(2016–620–000-001-010)The National Key Technology R&D Program(2014BAD11B0203)
文摘Background： With the rapid development of genomics, many functional genes have been targeted. Molecular marker assisted selection can accelerate the breeding process by linking selection to functional genes. Methods： In a study of upland cotton （Gossypium hirsutum L.）, the F2 segregated population was constructed by crossing X1570 （short branches） with Ekangmian 13 （long branches） to identify the short fruiting branch gene and marker assisted selection with SNP（Single Nucleotide Polymorphisms, SNP） linked to its trait. Result： The result demonstrated that linked SSR marker BNL3232 was screened by BSA（Bulked segregant analysis, BSA） method; one SNP locus was found, which was totally separated from the fruiting branches trait in upland cotton. Conclusion： It was verified that this SNP marker could be used for molecular assisted selection of cotton architecture
