Petroleum exploitation enriches the sulfonamide resistance gene sul2 in off shore sediments

Antibiotic resistance genes(ARGs)have been considered as emerging contaminants in nature owing to their wide distribution and human health risk.Anthropogenic activities can increase the diversity and abundance of ARGs and promote their spread in environment.Offshore environment is affected by multiple types of anthropogenic activities,of which excessive accumulation of petroleum substances poses a serious threat.Our previous experimental study has demonstrated that petroleum can increase the abundance of sulfonamide resistance genes(SRGs)in the seawater through horizontal gene transfer.However,the influence of petroleum substances on SRGs in offshore environment,especially adjacent the petroleum exploitation platform,is still unclear.Therefore,the effect of offshore oil exploitation on SRGs was investigated in the surface sediments collected from the Liaodong Bay,north China.The genes of sul1 and sul2 were present in all of the collected samples,while the sul3 gene was not detected in any sediments.The absolute abundance of sul2 gene in each sample was higher than sul1 gene.Class 1 integrons enhanced the maintenance and propagation of sul1 gene but not sul2 gene.More importantly,the results indicate that the absolute abundance of sul2 gene present in the offshore sediments that affected by petroleum exploitation was significantly higher than those in control.These findings provided direct evidence that offshore oil exploitation can influence the propagation of SRGs and implied that a more comprehensive risk assessment of petroleum substances to public health risks should be conducted.

Fe-MOF Derived Ferrous Hierarchically Porous Carbon Used as EF Cathode for PFOA Degradation

Pentadecafluorooctanoic acid (PFOA) is environmentally persistent, bioaccumulative, globally distributed and dangerous to human beings. Thus, the degradation of PFOA with effective method remains further exploration. Here, an Electro-Fenton (EF) system was studied for efficient PFOA degradation, and where a new composite material ferrous hierarchically porous carbon (FHPC) prepared by high temperature activation of MIL-100 (Fe) was applied as the cathode, and 81.4% PFOA (Initial 50 mg/L) elimination was achieved at a low potential of ?0.4 V (pH = 7, 3 h). With the increasing of the activated temperature, the catalytic ability of the materials is decreasing because the reduced surface area reduced and the iron nanoparticles size enlarged. Moreover, the H2O2 and the ?OH were also detected to confirm the dominating contribution of Electro-Fenton mechanism in the PFOA degradation. Thus, this material could be used in efficient heterogeneous EF technology for PFOA elimination.

Adsorption of Sulfamethoxazole on Nanoporous Carbon Derived from Metal-Organic Frameworks

Nanoporous Carbon (NPC) with high surface area of 1379 M2/G and high proportion of micropore and mesopore volume of 2.90 Cm3/G was prepared by carbonization of metal?organic frameworks ZIF-8. The adsorption of NPC towards the representative sulfonamide antibiotics sulfamethoxazole (SMX) from aqueous solutions was explored, in comparison with powder active carbon (AC). The adsorption kinetics and isotherms showed that the maximum adsorption capacity (Qm) of NPC toward SMX was 757 Mg/G, around 2 times than that of AC adsorption. The high adsorption affinity of NPC was related to the high surface area and special Mic/Mesopore structure. The pore-filling mechanism as well as electrostatic interaction had important influence on the high adsorption of NPC. The results implied that nanoporous carbon derived from mofs could remove the contaminants from aqueous solutions effectively, and would be a promising adsorbent for the removal of contaminants in the future.

Prediction model on hydrolysis kinetics of phthalate monoester:A density functional theory study

As primary degradation products of phthalate esters,phthalate monoesters(MPEs)have been widely detected in various aquatic environments and drawn growing toxicological concerns.Hydrolysis kinetics that is of importance for assessing environmental persistence of chemicals remain elusive for MPEs.Herein,kinetics of base-catalyzed and neutral hydrolysis for 18 MPEs with different leaving groups was investigated by density functional theory calculation.Results indicate that MPEs with leaving groups having p Kaof<10 prefer dissociative transition states.MPEs are more persistent than their parents,and their hydrolysis half-lives were calculated to vary from 3.4 min to 79.2 years(p H=7–9).A quantitative structure-activity relationship model was developed for predicting the hydrolysis kinetics parameters.It was found that p Kaof the leaving groups and electronegativity of the MPEs are key factors determining the hydrolysis kinetics.This work may lay a theoretical foundation for better understanding the chemical process that governs MPE persistence in aquatic environments.

Enhanced electrochemical oxidation of perfluorooctanoic acid on Ti/SnO_(2)-Sb electrode by surface morphology regulation

Electrochemical oxidation is an effective method to degrade persistent organic pollutants.However,due to the limited catalytic activity of traditional thin film electrodes,the anodic oxidation process is slow and usually requires high energy consumption.Herein,Ti/SnO_(2)-Sb electrode with regulated surface structure was reported to enhance the performance for electrochemical oxidation of persistent organic pollutants.The electrode deposited with SnO_(2)-Sb nanoneedles(Ti/N-SnO_(2)-Sb)showed higher oxidation activity.Its kinetic constant for perfluorooctanoic acid(PFOA)oxidation was 2.0 h^(-1)and the total organic carbon removal rate was 81.7%(4 h)at a relatively low current density of 6 mA/cm^2.Compared with Ti/SnO_(2)-Sb thin film and nanoparticles,Ti/N-SnO_(2)-Sb significantly improved the electrochemical active area and·OH yield,and simultaneously reduced the electron transfer resistance,which enabled it to oxidize PFOA more rapidly even at a lower potential.This work provides a new strategy for promoting the electrochemical oxidation performance.

Enhanced removal of high-As(Ⅲ)from Cl(-Ⅰ)-diluted SO_(4)(-Ⅱ)-rich wastewater at pH 2.3 via mixed tooeleite and(Cl(-Ⅰ)-free)ferric arsenite hydroxychloride formation

An advanced cost-saving method of removal of high-As(Ⅲ)from SO_(4)(-Ⅱ)-rich metallurgical wastewater has been developed by diluting the SO_(4)(-Ⅱ)content with As(Ⅲ)-Cl(-Ⅰ)-rich metallurgical wastewater and then by the direct precipitation of As(Ⅲ)with Fe(Ⅲ)at pH 2.3.As(Ⅲ)removal at various SO_(4)(-Ⅱ)/Cl(-Ⅰ)molar ratios and temperatures was investigated.The results showed that 65.2–98.2%of As(Ⅲ)immobilization into solids occurred at the SO_(4)(-Ⅱ)/Cl(-I)molar ratios of 1:1–32 and 15–60℃in 3 days,which were far higher than those in aqueous sole SO4(-Ⅱ)or Cl(-Ⅰ)media at the equimolar SO_(4)(-Ⅱ)or Cl(-Ⅰ)and the same temperature.SO_(4)(-Ⅱ)/Cl(-Ⅰ)molar ratio of 1:4 and 25℃were optimal conditions to reach the As removal maximum.Mixed aqueous SO4(-Ⅱ)and Cl(-Ⅰ)played a synergetic role in the main tooeleite formation together with(Cl(-Ⅰ)-free)ferric arsenite hydroxychloride(FAHC)involving the substitution of AsO_(3)^(3−)for Cl(-Ⅰ)for enhanced As fixation.The competitive complexation among FeH_(2)AsO_(3)^(2+),FeSO_(4)^(+)and FeCl^(2+)complexes was the main mechanism for the maximum As(Ⅲ)precipitation at the SO4(-Ⅱ)/Cl(-I)molar ratio of 1:4.Low As(Ⅲ)immobilization at high temperature with increased Fe(Ⅲ)hydrolysis was due to the formation of As(Ⅲ)-bearing ferrihydrite with the relatively high Fe/As molar ratio at acidic pH.

Effective adsorption of sulfamethoxazole, bisphenol A and methyl orange on nanoporous carbon derived from metal-organic frameworks

Nanoporous carbons(NPCs) derived from metal–organic frameworks(MOFs) are attracting increasing attention in many areas by virtue of their high specific surface area, large pore volume and unique porosity. The present work reports the preparation of an NPC with high surface area(1731 m^2/g) and pore volume(1.68 cm^3/g) by direct carbonization of MOF-5. We examined the adsorption of three typical contaminants from aqueous solutions, i.e., sulfamethoxazole(SMX),bisphenol A(BPA) and methyl orange(MO), by using the as-prepared NPC. The results demonstrated that NPC could adsorb the contaminants effectively, with adsorption capacity(qm) of 625 mg/g(SMX), 757 mg/g(BPA) and 872 mg/g(MO), respectively. These values were approximately 1.0-3.2 times higher than those obtained for single-walled carbon nanotubes(SWCNTs) and commercial powder active carbon(PAC) under the same conditions. With its high surface area and unique meso/macropore structure, the enhanced adsorption of NPC most likely originates from the cooperative interaction of a pore-filling mechanism, electrostatic interaction,and hydrogen bonding. In particular, the p H value has a crucial impact on adsorption, suggesting the significant contribution of electrostatic interaction between NPC and the contaminants. This study provides a proof-of-concept demonstration of MOF-derived nanoporous carbons as effective adsorbents of contaminants for water treatment.

Seasonal variations of soil bacterial communities in Suaeda wetland of Shuangtaizi River estuary, Northeast China

Estuarine wetland is the transitional interface linking terrestrial with marine ecosystems,and wetland microbes are crucial to the biogeochemical cycles of nutrients.The soil samples were collected in four seasons(spring,S1;summer,S2;autumn,S3;and winter,S4)from Suaeda wetland of Shuangtaizi River estuary,Northeast China,and the variations of bacterial community were evaluated by high-throughput sequencing.Soil properties presented a significant seasonal change,including p H,carbon(C)and total nitrogen(TN),and the microbial diversity,richness and structure also differed with seasons.Canonical correspondence analysis(CCA)and Mantel tests implied that soil p H,C and TN were the key factors structuring the microbial community.Gillisia(belonging to Bacteroidetes)and Woeseia(affiliating with Gammaproteobacteria)were the two primary components in the rhizosphere soils,displaying opposite variations with seasons.Based on PICRUSt(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States)prediction,the xenobiotics biodegradation related genes exhibited a seasonal decline,while the majority of biomarker genes involved in nitrogen cycle showed an ascending trend.These findings could advance the understanding of rhizosphere microbiota of Suaeda in estuarine wetland.

Electro-conductive crosslinked polyaniline/carbon nanotube nanofiltration membrane for electro-enhanced removal of bisphenol A

Nanofiltration(NF)has attracted increasing attention for wastewater treatment and potable water purification.However,the high-efficiency removal of micropollutants by NF membranes is a critical challenge.Owing to the adsorption and subsequent diffusion,some weakly charged or uncharged micropollutants,such as bisphenol A(BPA),can pass through NF membranes,resulting in low removal rates.Herein,an effective strategy is proposed to enhance the BPA removal efficiency of a crosslinked polyaniline/carbon nanotube NF membrane by coupling the membrane with electro-assistance.The membrane exhibited a 31.9%removal rate for 5 mg/L BPA with a permeance of 6.8 L/(m2·h·bar),while the removal rate was significantly improved to 98.1%after applying a voltage of 2.0 V to the membrane.Furthermore,when BPA coexisted with humic acid,the membrane maintained 94%removal of total organic carbon and nearly 100%removal of BPA at 2.0 V over the entire filtration period.Compared to continuous voltage applied to the membrane,an intermittent voltage(2.0 V for 0.5 h with an interval of 3.5 h)could achieve comparable BPA removal efficiency,because of the combined effect of membrane adsorption and subsequent electrochemical oxidation.Density functional theory calculations and BPA oxidation process analyses suggested that BPA was adsorbed by two main interactions:π–πand hydrogen-bond interactions.The adsorbed BPA was further electro-degraded into small organic acids or mineralized to CO_(2) and H2O.This work demonstrates that NF membranes coupled with electro-assistance are feasible for improving the removal of weakly charged or uncharged micropollutants.

Development and assessment of quantitative structure-activity relationship models for bioconcentration factors of organic pollutants

Bioconcentration factors (BCFs) are of great importance for ecological risk assessment of organic chemicals. In this study, a quantitative structure-activity relationship (QSAR) model for fish BCFs of 8 groups of compounds was developed employing partial least squares (PLS) regression, based on linear solvation energy relationship (LSER) theory and theoretical molecular structural descriptors. The guidelines for development and validation of QSAR models proposed by the Organization for Economic Cooperation and Development (OECD) were followed. The model results show that the main factors governing logBCF are Connolly molecular area (CMA), average molecular polarizability (α) and molecular weight (MW). Thus molecular size plays a critical role in affecting the bioconcentration of organic pollutants in fish. For the established model, the multiple correlation coefficient square (RY2) = 0.868, the root mean square error (RMSE) = 0.553 log units, and the leave-many-out cross-validated Q2CUM = 0.860, indicating its good goodness-of-fit and robustness. The model predictivity was evaluated by external validation, with the external explained variance (QE2XT) = 0.755 and RMSE = 0.647 log units. Moreover, the applicability domain of the developed model was assessed and visualized by the Williams plot. The developed QSAR model can be used to predict fish logBCF for organic chemicals within the application domain.

A critical review on thermodynamic mechanisms of membrane fouling in membrane-based water treatment process

Membrane technology is widely regarded as one of the most promising technologies for wastewater treatment and reclamation in the 21st century. However, membrane fouling significantly limits its applicability and productivity. In recent decades, research on the membrane fouling has been one of the hottest spots in the field of membrane technology. In particular, recent advances in thermodynamics have substantially widened people’s perspectives on the intrinsic mechanisms of membrane fouling. Formulation of fouling mitigation strategies and fabrication of anti-fouling membranes have both benefited substantially from those studies. In the present review, a summary of the recent results on the thermodynamic mechanisms associated with the critical adhesion and filtration processes during membrane fouling is provided. Firstly, the importance of thermodynamics in membrane fouling is comprehensively assessed. Secondly, the quantitative methods and general factors involved in thermodynamic fouling mechanisms are critically reviewed. Based on the aforementioned information, a brief discussion is presented on the potential applications of thermodynamic fouling mechanisms for membrane fouling control. Finally, prospects for further research on thermodynamic mechanisms underlying membrane fouling are presented. Overall, the present review offers comprehensive and in-depth information on the thermodynamic mechanisms associated with complex fouling behaviors, which will further facilitate research and development in membrane technology.

Comparison of CNT-PVA membrane and commercial polymeric membranes in treatment of emulsified oily wastewater

Membrane separation is an attractive technique for removal of emulsified oily wastewater.However,polymeric membranes which dominate the current market usually suffer from severe membrane fouling.Therefore,membranes with high fouling resistance are imperative to treat emulsified oily wastewater.In this study,carbon nanotube-polyvinyl alcohol(CNT-PVA)membrane was fabricated.And its separation performance for emulsified oily wastewater was compared with two commercial polymeric membranes(PVDF membrane and PES membrane)by filtration of two homemade emulsions and one cutting fluid emulsion.The results show that these membranes have similar oil retention efficiencies for the three emulsions.Whereas,the permeation flux of CNT-PVA membrane is 1.60 to 3.09 times of PVDF membrane and 1.41 to 11.4 times of PES membrane,respectively.Moreover,after five consecutive operation circles of filtration process and back flush,CNT-PVA membrane can recover 62.3% to 72.9% of its initial pure water flux.However,the pure water flux recovery rates are only 24.1% to 35.3% for PVDF membrane and 6.0% to 26.3% for PES membrane,respectively.Therefore,CNT-PVA membrane are more resistant to oil fouling compared with the two polymeric membranes,showing superior potential in treatment of emulsified oily wastewater.

Performance and microbial community analysis of bioaugmented activated sludge for nitrogen-containing organic pollutants removal

Nitrogen-containing organic pollutants(quinoline,pyridine and indole)are widely distributed in coking wastewater,and bioaugmentation with specific microorganisms may enhance the removal of these recalcitrant pollutants.The bioaugmented system(group B)was constructed through inoculation of two aromatics-degrading bacteria,Comamonas sp.Z1(quinoline degrader)and Acinetobacter sp.JW(indole degrader),into the activated sludge for treatment of quinoline,indole and pyridine,and the non-bioaugmented activated sludge was used as the control(group C).Both groups maintained high efficiencies(>94%)for removal of nitrogen-containing organic pollutants and chemical oxygen demand(COD)during the long-term operation,and group B was highly effective at the starting period and the operation stage fed with raw wastewater.High-throughput sequencing analysis indicated that nitrogen-containing organic pollutants could shape the microbial community structure,and communities of bioaugmented group B were clearly separated from those of non-bioaugmented group C as observed in non-metric multidimensional scaling(NMDS)plot.Although the inoculants did not remain their dominance in group B,bioaugmentation could induce the formation of effective microbial community,and the indigenous microbes might play the key role in removal of nitrogen-containing organic pollutants,including Dokdonella,Comamonas and Pseudoxanthomonas.Phylogenetic Investigation of Communities by Reconstruction of Unobserved States(PICRUSt)analysis suggested that bioaugmentation could facilitate the enrichment of functional genes related to xenobiotics biodegradation and metabolism,probably leading to the improved performance in group B.This study indicated that bioaugmentation could promote the removal of nitrogen-containing organic pollutants,which should be an effective strategy for wastewater treatment.

the National Natural Science Foundation of China(41877029,41961130383);Royal SocietyNewton Advanced Fellowship(NAF\R1\191017);Wuhan Science and Technology Bureau(2019020701011469).

Due to the tremendous diversity of microbial organisms in topsoil,the estimation of saturated richness in a belowground ecosystem is still challenging.Here,we intensively surveyed the 16S rRNA gene in four 1 m2 sampling quadrats in a typical grassland,with 141 biological or technical replicates generating over 11 million sequences per quadrat.Through these massive data sets and using both non-asymptotic extrapolation and non-parametric asymptotic approaches,results revealed that roughly 15919±193,27193±1076 and 56985±2347 prokaryotic species inhabited in 1 m2 topsoil,classifying by DADA2,UPARSE(97%cutoff)and Deblur,respectively,and suggested a huge difference among these clustering tools.Nearly 500000 sequences were required to catch 50%species in 1 m2,while any estimator based on 500000 sequences would still lose about a third of total richness.Insufficient sequencing depth will greatly underestimate both observed and estimated richness.At least~911000,~3461000,and~1878000 sequences were needed for DADA2,UPARSE,and Deblur,respectively,to catch 80%species in 1 m2 topsoil,and the numbers of sequences would be nearly twice to three times on this basis to cover 90%richness.In contrast,α-diversity indexes characterized by higher order of Hill numbers,including Shannon entropy and inverse Simpson index,reached saturation with fewer than 100000 sequences,suggesting sequencing depth could be varied greatly when focusing on exploring differentα-diversity characteristics of a microbial community.Our findings were fundamental for microbial studies that provided benchmarks for the extending surveys in large scales of terrestrial ecosystems.

Conductive and stable polyphenylene/CNT composite membrane for electrically enhanced membrane fouling mitigation

Nanocarbon-based conductive membranes, especially carbon nanotube (CNT)-based membranes, have tremendous potential for wastewater treatment and water purification because of their excellent water permeability and selectivity, as well as their electrochemically enhanced performance (e.g., improved antifouling ability). However, it remains challenging to prepare CNT membranes with high structural stability and high electrical conductivity. In this study, a highly electroconductive and structurally stable polyphenylene/CNT (PP/CNT) composite membrane was prepared by electropolymerizing biphenyl on a CNT hollow fiber membrane. The PP/CNT membrane showed 3.4 and 5.0 times higher electrical conductivity than pure CNT and poly(vinyl alcohol)/CNT (PVA/CNT) membranes, respectively. The structural stability of the membrane was superior to that of the pure CNT membrane and comparable to that of the PVA/CNT membrane. The membrane fouling was significantly alleviated under an electrical assistance of −2.0 V, with a flux loss of only 11.7% after 5 h filtration of humic acid, which is significantly lower than those of PP/CNT membranes without electro-assistance (56.8%) and commercial polyvinylidene fluoride (PVDF) membranes (64.1%). Additionally, the rejection of negatively charged pollutants (humic acid and sodium alginate) was improved by the enhanced electrostatic repulsion. After four consecutive filtration-cleaning cycle tests, the flux recovery rate after backwashing reached 97.2%, which was much higher than those of electricity-free PP/CNT membranes (67.0%) and commercial PVDF membranes (61.1%). This study offers insights into the preparation of stable conductive membranes for membrane fouling control in potential water treatment applications.

Solar evaporation for simultaneous oil-water separation and electricity generation with Janus wood-based absorbers

Oily wastewater from ocean oil spills endangers marine ecosystems and human health. Therefore, developing an effective and sustainable solution for separating oil-water mixtures is urgent. Interfacial solar photothermal evaporation is a promising approach for the complete separation of two-phase mixtures using only solar energy. Herein, we report a carbonized wood-based absorber with Janus structure of comprising a hydrophobic top-layer and an oleophobic bottom-layer for simultaneous solar-driven oil-water separation and electricity generation. Under sunlight irradiation, the rapid evaporation of seawater will induce a separation of oil-water mixtures, and cause a high salt concentration region underlying the interface, while the bottom “bulk water” maintains in a low salt concentration, thus forming a salinity gradient. Electricity can be generated by salinity gradient power. Therefore, oil-water separation efficiency of > 99% and derived extra electricity power of ~0.1 W/m2 is achieved under solar radiation, demonstrating the feasibility of oil-water separation and electricity production synchronously directly using solar energy. This work provides a green and cost-effective path for the separation of oil-water mixtures.

Multistep conversion of cresols by phenol hydroxylase and 2,3-dihydroxy-biphenyl 1,2-dioxygenase

一个 multistep 变换系统酚 hydroxylase 创作了(PH < 潜水艇 class= “ a-plus-plus ”> IND )和 2,3-dihydroxy-biphenyl 1,2-dioxygenase ( BphC <潜水艇class=“ a-plus-plus ”> LA-4 )被用来综合从o甲酚和m甲酚的 methylcatechols 和 semialdehydes 第一次。停靠 PyMOL 显示的研究预言甲酚和 methylcatechols 能被这个 multistep 变换系统理论上转变。高效液体层析团 spectrometry (HPLCMS ) 分析也显示从 multistep 变换形成的产品是相应 3-methylcatechol， 4-methylcatechol， 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoic 酸(2-hydroxy-3-methyl-ODA ) 和 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoic 酸(2-hydroxy-5-methyl-ODA ) 。recombinant E 的最佳的房间集中。表示酚 hydroxylase 的 coli 紧张 BL21 ( DE3 )( PH <潜水艇class=“ a-plus-plus ”> IND )和 2,3-dihydroxy-biphenyl 1,2-dioxygenase ( BphC <潜水艇class=“ a-plus-plus ”>为o甲酚和m甲酚的 multistep 变换的 LA-4 )和 pH 是 4.0 (接地模仿的 Ce ( IV )抽取出现了类似为高 Ce ( IV )集中和酸味大小写的趋势。模型结果显示高起始的 Ce (IV ) 集中和酸味两个都在 Ce (IV ) 抽取上有有害效果并且脱衣。在膜和散开层在之间的厚度的 Ce (IV ) 的散开系数喂阶段和膜阶段被获得，价值是 6.31 5 ?% 并且 9.0，分别地) ，但是没在降级上有效果新鲜， non-predegraded，文档(% BD？？

Utilizing transparent and conductive SnO2 as electron mediator to enhance the photocatalytic performance of Z-scheme Si-SnO2-TiOx

Z-scheme photocatalysts,with strong redox ability,have a great potential for pollutants degradation.However,it is challenging to construct efficient Z-scheme photocatalysts because of their poor interfacial charge separation.Herein,by employing transparent and conductive SnO2 as electron mediator to pass light through and promote interfacial charge transportation,a novel Z-scheme photocatalyst Si-SnO2-TiOx(i<x<2)was constructed.The Z-scheme photocatalyst displayed an order of magnitude higher photocurrent density and a 4-fold increase in open-circuit potential compared to those of Si.Moreover,the onset potential shifted negatively for approximately 2.2 V.Benefiting from these advantages,this Z-scheme Si-SnO2-TiOx exhibited efficient photocatalytic performance toward phenol degradation and mineralization.15%of the phenol was degraded without bias potential and 70%of the TOC was removed during phenol degradation.Other typical pollutants such as bisphenol A and atrazine could also be degraded without bias potential.Introducing a transparent and conductive electron mediator to construct Z-scheme photocatalyst gives a new sight to the improvement of photocatalytic performance in Z scheme.

Biodegradation of indole by a newly isolated Cupriavidus sp. SHE

Indole, a typical nitrogen heterocyclic aromatic pollutant, is extensively spread in industrial wastewater. Microbial degradation has been proven to be a feasible approach to remove indole, whereas the microbial resources are fairly limited. A bacterial strain designated as SHE was isolated and found to be an efficient indole degrader. It was identified as Cupriavidus sp. according to 16 SrRNA gene analysis. Strain SHE could utilize indole as the sole carbon source and almost completely degrade 100 mg/L of indole within 24 hr. It still harbored relatively high indole degradation capacity within p H 4–9 and temperature 25°C–35°C. Experiments also showed that some heavy metals such as Mn2+, Pb2+and Co2+did not pose severe inhibition on indole degradation. Based on high performance liquid chromatography–mass spectrum analysis, isatin was identified as a minor intermediate during the process of indole biodegradation. A major yellow product with m/z 265.0605(C15H8N2O3) was generated and accumulated, suggesting a novel indole conversion pathway existed. Genome analysis of strain SHE indicated that there existed a rich set of oxidoreductases, which might be the key reason for the efficient degradation of indole. The robust degradation ability of strain SHE makes it a promising candidate for the treatment of indole containing wastewater.