In the past two decades,various super-resolution(SR)microscopy techniques have been developed to break the di®raction limit using subdi®raction excitation to spatially modulate the°uorescence emission.P...In the past two decades,various super-resolution(SR)microscopy techniques have been developed to break the di®raction limit using subdi®raction excitation to spatially modulate the°uorescence emission.Photomodulatable°uorescent proteins(FPs)can be activated by light of speci¯c wavelengths to produce either stochastic or patterned subdi®raction excitation,resulting in improved optical resolution.In this review,we focus on the recently developed photomodulatable FPs or commonly used SR microscopies and discuss the concepts and strategies for optimizing and selecting the biochemical and photophysical properties of PMFPs to improve the spatiotemporal resolution of SR techniques,especially time-lapse live-cell SR techniques.展开更多
With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncodi...With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncoding RNAs （lncRNAs） composing the major part of the transcriptome have been identified （Ravasi et al., 2006; Birney et al., 2007;展开更多
基金This project was supported by the National Basic Research Program (2013CB910103)the National Natural Science Foundation of China (Grant Nos.31421002,31370851 and 31300612)+1 种基金the Project of the Chinese Academy of Sciences (XDB08030202)the Beijing Natural Science Foundation (7131011)。
文摘In the past two decades,various super-resolution(SR)microscopy techniques have been developed to break the di®raction limit using subdi®raction excitation to spatially modulate the°uorescence emission.Photomodulatable°uorescent proteins(FPs)can be activated by light of speci¯c wavelengths to produce either stochastic or patterned subdi®raction excitation,resulting in improved optical resolution.In this review,we focus on the recently developed photomodulatable FPs or commonly used SR microscopies and discuss the concepts and strategies for optimizing and selecting the biochemical and photophysical properties of PMFPs to improve the spatiotemporal resolution of SR techniques,especially time-lapse live-cell SR techniques.
基金supported by the grants from the National Natural Science Foundation of China (No. 31300889)
文摘With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncoding RNAs （lncRNAs） composing the major part of the transcriptome have been identified （Ravasi et al., 2006; Birney et al., 2007;
