Membranous nephropathy （MN） is the most common cause of nephrotic syndrome in adults.The renal biopsy is the gold standard for the disease diagnosis,but is invasive.M-type phospholipase A2 receptor （PLA2R） on huma...Membranous nephropathy （MN） is the most common cause of nephrotic syndrome in adults.The renal biopsy is the gold standard for the disease diagnosis,but is invasive.M-type phospholipase A2 receptor （PLA2R） on human podocytes could serve as the target for podocytopathic antibodies （anti-PLA2R）.1 Patients with iMN （70%-80%） were found the circulating antibodies against PLA2R.1 And the podocyte-secreted angiopoietinlike-4 （Angptl4） could be another key role in nephrotic syndrome3.展开更多
Background:Leukocyte telomere length shortening is a characteristic of premature senescence,a process that can be accelerated by oxidative stress.In general,patients with end-stage renal disease undergoing regular hem...Background:Leukocyte telomere length shortening is a characteristic of premature senescence,a process that can be accelerated by oxidative stress.In general,patients with end-stage renal disease undergoing regular hemodialysis(HD)are repeatedly exposed to oxidative stress.Patients undergoing HD tend to have cardiovascular diseases associated with oxidative stress and inflammation.Therefore,we assumed that telomere length is associated with HD vintage and the degree of vascular calcification.Methods:A total of 144 patients undergoing regular HD before kidney transplantation and 62 patients on hemodialysis,but not undergoing kidney transplantation,were enrolled.We measured common laboratory values,such as calcium,phosphate,and hemoglobin levels,and assessed the degree of vascular calcification in the patients.The leukocyte telomere length was measured using reverse transcription polymerase chain reaction,and Spearman correlation was used for correlation analysis.Results:The leukocyte telomere length was negatively associated with age(rho=-0.306,P<0.01);it was shorter in middle-aged patients than in young patients(13.48±4.80 vs.15.86±4.51,P<0.01).The telomere length was significantly different among patients aged 52-74 years in groups with different HD vintages.Additionally,the telomere length was positively associated with serum hemoglobin(Hb)levels in all patients(rho=0.290,P<0.01).There was a significant difference among patients divided into three groups according to the degree of anemia(17.09±5.64 vs.14.40±4.07 vs.13.99±3.95,P<0.01).Further,a significant difference was observed in the telomere length among patients with different degrees of vascular calcification(16.79±4.91 vs.13.61±2.82 vs.14.62±3.63 vs.10.71±3.74,P<0.01).The telomere length was shorter in the patients on hemodialysis who did not receive a kidney transplant than in the surgical patients(8.12±1.83 vs.14.33±4.63,P<0.01).Conclusion:This study demonstrated that the telomere length was significantly correlated with HD vintage in patients of a certain age group.The telomere length was shorter in patients on hemodialysis who matched for age and dialysis vintage with kidney transplant patients.It was also associated with vascular calcification and serum Hb levels in all patients undergoing HD.展开更多
基金This study was supported by the grants from the National Nature Science Foundation of China （No. 2011BAI10B07, No. 2012CB517603, and No. 2012AA02A512）.
文摘Membranous nephropathy （MN） is the most common cause of nephrotic syndrome in adults.The renal biopsy is the gold standard for the disease diagnosis,but is invasive.M-type phospholipase A2 receptor （PLA2R） on human podocytes could serve as the target for podocytopathic antibodies （anti-PLA2R）.1 Patients with iMN （70%-80%） were found the circulating antibodies against PLA2R.1 And the podocyte-secreted angiopoietinlike-4 （Angptl4） could be another key role in nephrotic syndrome3.
文摘Background:Leukocyte telomere length shortening is a characteristic of premature senescence,a process that can be accelerated by oxidative stress.In general,patients with end-stage renal disease undergoing regular hemodialysis(HD)are repeatedly exposed to oxidative stress.Patients undergoing HD tend to have cardiovascular diseases associated with oxidative stress and inflammation.Therefore,we assumed that telomere length is associated with HD vintage and the degree of vascular calcification.Methods:A total of 144 patients undergoing regular HD before kidney transplantation and 62 patients on hemodialysis,but not undergoing kidney transplantation,were enrolled.We measured common laboratory values,such as calcium,phosphate,and hemoglobin levels,and assessed the degree of vascular calcification in the patients.The leukocyte telomere length was measured using reverse transcription polymerase chain reaction,and Spearman correlation was used for correlation analysis.Results:The leukocyte telomere length was negatively associated with age(rho=-0.306,P<0.01);it was shorter in middle-aged patients than in young patients(13.48±4.80 vs.15.86±4.51,P<0.01).The telomere length was significantly different among patients aged 52-74 years in groups with different HD vintages.Additionally,the telomere length was positively associated with serum hemoglobin(Hb)levels in all patients(rho=0.290,P<0.01).There was a significant difference among patients divided into three groups according to the degree of anemia(17.09±5.64 vs.14.40±4.07 vs.13.99±3.95,P<0.01).Further,a significant difference was observed in the telomere length among patients with different degrees of vascular calcification(16.79±4.91 vs.13.61±2.82 vs.14.62±3.63 vs.10.71±3.74,P<0.01).The telomere length was shorter in the patients on hemodialysis who did not receive a kidney transplant than in the surgical patients(8.12±1.83 vs.14.33±4.63,P<0.01).Conclusion:This study demonstrated that the telomere length was significantly correlated with HD vintage in patients of a certain age group.The telomere length was shorter in patients on hemodialysis who matched for age and dialysis vintage with kidney transplant patients.It was also associated with vascular calcification and serum Hb levels in all patients undergoing HD.
