Peptides (molecular weights are 1200 – 11,600 Da) biologically acting at concentration 10-8 - 10-15 mg/ml were obtained from leaves of dill Anethum graveolens L. and celandine Chelidonium majus L. by circular dichroi...Peptides (molecular weights are 1200 – 11,600 Da) biologically acting at concentration 10-8 - 10-15 mg/ml were obtained from leaves of dill Anethum graveolens L. and celandine Chelidonium majus L. by circular dichroism (CD) spectroscopy. We have shown that these peptides form second structure characterized by the prevalence of β-structures and coil in water solution. In model of organotypic roller culturing of triton liver in vitro, the ability of the peptides isolated from Anethum graveolens L. and Chelidonium majus L. at concentration 10-8 - 10-15 mg/ml to influence increasing of pigment cells, which provided protective function of organism, was demonstrated.展开更多
The three-dimensional organization of the genome is closely related to its functioning. Interactions between parts of the genome located at large distances from each other have been detected within the chromosomes of ...The three-dimensional organization of the genome is closely related to its functioning. Interactions between parts of the genome located at large distances from each other have been detected within the chromosomes of different organisms, which led to the discovery of topologically associated domains (TADs). Methods that reveal such interactions between chromosomal loci imply detection of both protein-protein and protein-DNA interactions. We investigated the possibility of involvement of the direct DNA-DNA interactions in the structural and functional organization of Drosophila melanogaster chromosomal 87A7 locus, containing genes hsp70Aa and hsp70Ab, with the sequence analysis method. Our results indicate that the functional organization of 87A7 locus may involve different elements: chromosomal DNA fragments that attach chromosomes to the nuclear envelope, short polypurine/polypyrimidine tracts, insulators and their proteins. The combination of interactions of these elements may cause different functional states of 87A7 locus.展开更多
Dear Editor,Chromosomal translocations result from the interchange of geneticmaterial between non-homologous chromosomes.Chromosomal translocations are formed by erroneous repair of double-stranded breaks(DSBs)via non...Dear Editor,Chromosomal translocations result from the interchange of geneticmaterial between non-homologous chromosomes.Chromosomal translocations are formed by erroneous repair of double-stranded breaks(DSBs)via non-homologous end joining(NHEJ)[1].Some genotoxic drugs produce DSBs and thus present a major risk factor for the development of oncogenic chromosomal translocations.The risk factors that interfere with translocationprone DSB repair,once DSBs are already formed,are obscure,and potential effects of drugs on translocation formation during this step have never been explored.展开更多
文摘Peptides (molecular weights are 1200 – 11,600 Da) biologically acting at concentration 10-8 - 10-15 mg/ml were obtained from leaves of dill Anethum graveolens L. and celandine Chelidonium majus L. by circular dichroism (CD) spectroscopy. We have shown that these peptides form second structure characterized by the prevalence of β-structures and coil in water solution. In model of organotypic roller culturing of triton liver in vitro, the ability of the peptides isolated from Anethum graveolens L. and Chelidonium majus L. at concentration 10-8 - 10-15 mg/ml to influence increasing of pigment cells, which provided protective function of organism, was demonstrated.
文摘The three-dimensional organization of the genome is closely related to its functioning. Interactions between parts of the genome located at large distances from each other have been detected within the chromosomes of different organisms, which led to the discovery of topologically associated domains (TADs). Methods that reveal such interactions between chromosomal loci imply detection of both protein-protein and protein-DNA interactions. We investigated the possibility of involvement of the direct DNA-DNA interactions in the structural and functional organization of Drosophila melanogaster chromosomal 87A7 locus, containing genes hsp70Aa and hsp70Ab, with the sequence analysis method. Our results indicate that the functional organization of 87A7 locus may involve different elements: chromosomal DNA fragments that attach chromosomes to the nuclear envelope, short polypurine/polypyrimidine tracts, insulators and their proteins. The combination of interactions of these elements may cause different functional states of 87A7 locus.
基金Cancéropole IdF,the Koltzov Institute of Developmental Biology,Russian Academy of Sciences Government basic research programs(0088-2021-0007)the Ministry of Science and Higher Education grants(075-15-2021-1075 to YV and 075-15-2021-1062 and 075-15-2021-1060 to MR)+1 种基金Russian Foundation for Basic Research(19-54-16002)the Interdisciplinary Scientific and Educational School of Moscow University≪Molecular Technologies of the Living Systems and Synthetic Biology≫to MR.AS was supported by the Travel Grant from Boehringer Ingelheim Fonds in 2018.
文摘Dear Editor,Chromosomal translocations result from the interchange of geneticmaterial between non-homologous chromosomes.Chromosomal translocations are formed by erroneous repair of double-stranded breaks(DSBs)via non-homologous end joining(NHEJ)[1].Some genotoxic drugs produce DSBs and thus present a major risk factor for the development of oncogenic chromosomal translocations.The risk factors that interfere with translocationprone DSB repair,once DSBs are already formed,are obscure,and potential effects of drugs on translocation formation during this step have never been explored.
