Salmonella typhimurium purB encodes adeny-losuccinate lyase (ASL), the enzyme that catalyzes step 8 in the pathway for de novo synthesis of inosine 5’-monophos-phate (IMP) and also the final reaction in the two-step ...Salmonella typhimurium purB encodes adeny-losuccinate lyase (ASL), the enzyme that catalyzes step 8 in the pathway for de novo synthesis of inosine 5’-monophos-phate (IMP) and also the final reaction in the two-step sequence from IMP to adenosine monophosphate (AMP). The nucleotide sequence ofpurB was obtained by the genetic map and sequence homologous analysis. The conserved pur operator in purB was identified to be located 185 bp downstream of the initiation codon and overlaps codons 62 - 67 in the protein-coding region. The binding of PurR to this operator was demonstrated by gel retardation experiment and site-directed mutagenesis, indicating that the purB is under the control of purR. We also answered why previous study had conflicting report concerning the regulation of purB by purR by identifying the junction site of purB to lacZ in a purB-MudJ (lacZ, Kan’) fusion strain. This result strongly supports that the purB is the second gene in the ycfC-purB operon.展开更多
Cholelithiasis-induced cholestasis is one of the most common causes of hospitalization due to gastrointestinal disease,yet considerable knowledge gaps exist in the pathogenesis of this disease.This can partially be ex...Cholelithiasis-induced cholestasis is one of the most common causes of hospitalization due to gastrointestinal disease,yet considerable knowledge gaps exist in the pathogenesis of this disease.This can partially be explained by inadequate characterization of experimental cholestasis models.Here,we compared the transcriptional profile of commonly used mouse models for obstructive cholestasis and benchmarked them to human disease to identify the model(s)best suited for cholelithiasis-induced cholestasis research and to uncover conserved mechanisms involved in human and murine cholestasis.展开更多
基金Thiswork was supported by the National Natural Science Foundation of China.
文摘Salmonella typhimurium purB encodes adeny-losuccinate lyase (ASL), the enzyme that catalyzes step 8 in the pathway for de novo synthesis of inosine 5’-monophos-phate (IMP) and also the final reaction in the two-step sequence from IMP to adenosine monophosphate (AMP). The nucleotide sequence ofpurB was obtained by the genetic map and sequence homologous analysis. The conserved pur operator in purB was identified to be located 185 bp downstream of the initiation codon and overlaps codons 62 - 67 in the protein-coding region. The binding of PurR to this operator was demonstrated by gel retardation experiment and site-directed mutagenesis, indicating that the purB is under the control of purR. We also answered why previous study had conflicting report concerning the regulation of purB by purR by identifying the junction site of purB to lacZ in a purB-MudJ (lacZ, Kan’) fusion strain. This result strongly supports that the purB is the second gene in the ycfC-purB operon.
基金This work was supported by a doctoral grant strategic basic research with application number 1S47219N from Research Foundation-Flanders,Belgium and the Special Research Fund-UGent.
文摘Cholelithiasis-induced cholestasis is one of the most common causes of hospitalization due to gastrointestinal disease,yet considerable knowledge gaps exist in the pathogenesis of this disease.This can partially be explained by inadequate characterization of experimental cholestasis models.Here,we compared the transcriptional profile of commonly used mouse models for obstructive cholestasis and benchmarked them to human disease to identify the model(s)best suited for cholelithiasis-induced cholestasis research and to uncover conserved mechanisms involved in human and murine cholestasis.
