1,8-cineole, also known as eucalyptol, is the main constituent and the most important of the leaf oils of many species of the genus Eucalyptus. In present study, the essential oils isolated by hydrodistillation from t...1,8-cineole, also known as eucalyptol, is the main constituent and the most important of the leaf oils of many species of the genus Eucalyptus. In present study, the essential oils isolated by hydrodistillation from the leaves of Eucalyptus camaldulensis from six different locations were analyzed by GC-FID and GC-MS. Essential oil yields ranged from 0.33% to 4.35%. In total, 34 constituents were identified, accounting for 94.0% to 99.8% of the total compositions. 1,8-cineole (46.4% - 84.4%) was the main constituent of all the essential oil samples, except for one sample collected in the Fatick region, which had p-cymene (46.4%) and 1,8-cineole (26.0%) as major compounds. The essential oil showed excellent activity against S. aureus, E. coli and E. faecalis (IZ = 25.3 ± 1.2 mm;18.7 ± 0.6 mm;17.8 ± 0.3 mm, respectively) and moderate activity against P. aeruginosa (IZ = 10.8 ± 0.8 mm). They may have potential applications in food and pharmaceutical products.展开更多
Background: Extended-spectrum beta-lactamase (ESBL) producing bacteria are a real public health problem, particularly in Africa. Among these ESBLs, there are the Muenchen Cefotaximase (CTX-M) described all over the wo...Background: Extended-spectrum beta-lactamase (ESBL) producing bacteria are a real public health problem, particularly in Africa. Among these ESBLs, there are the Muenchen Cefotaximase (CTX-M) described all over the world of which the most frequent is the CTX-M of group 1 particularly the CTX-M-15 variant. The objective of this study was to determine the prevalence of CTX-M group 1 ESBL-producing Escherichia coli strains and to test their antibiotics susceptibility profile. Methodology: A retrospective cross-sectional descriptive study was conducted to detect ESBL-secreting Escherichia coli strains by the synergy test. Identification of CTX-M type ESBL from group 1 was performed using the NG-Test CTX-M rapid diagnostic test (NG-Biotech®). Antibiotic susceptibility profile was determined using CA-SFM/EUCAST guidelines 2019. Data entry and statistical analysis were performed with Excel version 2010 and SPSS 20.0 respectively. Results: Eighty-two ESBL-producing Escherichia coli strains were tested. A group 1 CTX-M ESBL was detected in 75.6% of the strains (n = 62). These strains were highly resistant to cefotaxim (100%), aztreonam (100%), ceftazidim (85.4%) and cefepim (66.1%). They were also resistant to quinolones, gentamycin and sulfadoxine-trimethoprim combination. However, these strains showed sensitivity to ertapenem (100%), cefoxitin (69.3%), tigecyclin (66%), and amikacin (66.1%). The combination of piperacillin and tazobactam was active on 30.6% of the strains against 6.4% for the combination of amoxicillin and clavulanic acid. Conclusion: The CTX-M type ESBL of group 1 was present in the majority of ESBL-producing Escherichia colis trains. Despite the production of this enzyme conferring resistance to most beta-lactam antibiotics, some antibiotics remain active to treat infections caused by these germs.展开更多
文摘1,8-cineole, also known as eucalyptol, is the main constituent and the most important of the leaf oils of many species of the genus Eucalyptus. In present study, the essential oils isolated by hydrodistillation from the leaves of Eucalyptus camaldulensis from six different locations were analyzed by GC-FID and GC-MS. Essential oil yields ranged from 0.33% to 4.35%. In total, 34 constituents were identified, accounting for 94.0% to 99.8% of the total compositions. 1,8-cineole (46.4% - 84.4%) was the main constituent of all the essential oil samples, except for one sample collected in the Fatick region, which had p-cymene (46.4%) and 1,8-cineole (26.0%) as major compounds. The essential oil showed excellent activity against S. aureus, E. coli and E. faecalis (IZ = 25.3 ± 1.2 mm;18.7 ± 0.6 mm;17.8 ± 0.3 mm, respectively) and moderate activity against P. aeruginosa (IZ = 10.8 ± 0.8 mm). They may have potential applications in food and pharmaceutical products.
文摘Background: Extended-spectrum beta-lactamase (ESBL) producing bacteria are a real public health problem, particularly in Africa. Among these ESBLs, there are the Muenchen Cefotaximase (CTX-M) described all over the world of which the most frequent is the CTX-M of group 1 particularly the CTX-M-15 variant. The objective of this study was to determine the prevalence of CTX-M group 1 ESBL-producing Escherichia coli strains and to test their antibiotics susceptibility profile. Methodology: A retrospective cross-sectional descriptive study was conducted to detect ESBL-secreting Escherichia coli strains by the synergy test. Identification of CTX-M type ESBL from group 1 was performed using the NG-Test CTX-M rapid diagnostic test (NG-Biotech®). Antibiotic susceptibility profile was determined using CA-SFM/EUCAST guidelines 2019. Data entry and statistical analysis were performed with Excel version 2010 and SPSS 20.0 respectively. Results: Eighty-two ESBL-producing Escherichia coli strains were tested. A group 1 CTX-M ESBL was detected in 75.6% of the strains (n = 62). These strains were highly resistant to cefotaxim (100%), aztreonam (100%), ceftazidim (85.4%) and cefepim (66.1%). They were also resistant to quinolones, gentamycin and sulfadoxine-trimethoprim combination. However, these strains showed sensitivity to ertapenem (100%), cefoxitin (69.3%), tigecyclin (66%), and amikacin (66.1%). The combination of piperacillin and tazobactam was active on 30.6% of the strains against 6.4% for the combination of amoxicillin and clavulanic acid. Conclusion: The CTX-M type ESBL of group 1 was present in the majority of ESBL-producing Escherichia colis trains. Despite the production of this enzyme conferring resistance to most beta-lactam antibiotics, some antibiotics remain active to treat infections caused by these germs.
