Detection of ECTV,MHV and SV in Clean-grade Mice and Rats in East China

To study the epidemic status of Ectromelia virus （ ECTV）, Mouse hepatitis virus （MHV） and Sendai virus （SV） in clean-grade experimental mice and rats in East China, 30 individuals of ICR and/or C57BL/6 mice and 15 individuals of SD or Wistar rats were purchased from four major experimental animal supply bases in East China. Blood samples were collected by eyeball picking method, and serum was separated. ECTV, MHV and SV serum antibodies in mice were de- teeted using imported commercial ELISA kits. Since rats were not easily infected by MHV and SV, only the ECTV serum antibody was detected in rats. Survey re- suits revealed that clean-grade experimental mice and rats derived from three out of four major experimental animal supply bases were negative in detection, but the positive rates of MHV and SV serum antibody in clean-grade ICR mice in one supply base were 56.7% and 100.0%, respectively, while the positive rate of MHV serum antibody in C57BL/6 mice was 100%. Research results showed that most of the commercial experimental mice and rats in major experimental animal supply bases in East China were qualified in detection of above three kinds of diseases, but MHV and SV still existed in individual base, with severe infection status. Thus, active measures must be strengthened to purify mice and rats. Meanwhile, monitoring of mouse and rat diseases must be developed seriously to guarantee the quality of experimental mice and rats, so as to avoid adveme impacts on breeding and use of experimental rats and miee.

Effect of Complex Preparation on the Hemorheological Indexes of Heat Stress Piglets

In the study, heat stress environment was simulated by artificial climate cabin to establish the heat stress model of piglets. A total of 64 2-month-old Chinese miniature pigs used for test were sdected and randomly divided into control group, heat stress group, complex preparation extract I treatment group and complex preparation extract II treatment group. Hematocrit was analyzed by Wintrobe test, meanwhile, the hemorheological indexes such as whole blood viscosity were analyzed by the automated analysis system of hemorrheology. In order to study the dynamic changes of complex preparation on the hemorheologieal indexes of heat stress piglets. The results showed that the piglets whole blood viscosity at high shear rate on the 10~ days in heat stress group was significantly highe~ than that in control group （P 〈 0.05） ; erythrocyte deformation indexes on the 6th and 10th days in heat stress group were significantly lower than that in control group （P 〈 0.05） ; hematocfit on the 1＂, 3＇~, 6~h and 10＇h days in heat stress group was significantly higher than that in control group （P 〈 0.05）. Hemorhealogieal inde- xes of the piglets in treatment group adding with complex preparation had been significantly restored, the effect on the 10th day after the stress was the most significant （P 〈 0.05）. The test results indicated that heat stress showed some significant influences on the health of piglets. Complex preparation could reduce the physiological damage causing by heat stress, and the effect of complex preparation II was better than that of complex preparation I.

Epidemic Status and Research Progress on Prevention of Rabbit Coccidiosis in China

Rabbit is not only an economic animal, but also an important experimental animal. Rabbit coccidiosis seriously affects development of rabbit industry and quality safety of experimental rabbits. Currently, infections of rabbit coccidiosis are very common in China, which mostly are mixed infections in clinical, with multiple species of coccidia. The current prevention against rabbit coccidiosis mainly depends on anticoccidial drugs, while live vaccine, subunit vaccines and ge- netic engineering vaccine for coccidia are developing.