The role of Ese-2,an Ets family transcription factor,in gene regulation is not known.In this study,the interactionbetween Ese-2 and cytokeratin 18(K18)intron 1 was characterized in lung epithelial cells.Reporter gene ...The role of Ese-2,an Ets family transcription factor,in gene regulation is not known.In this study,the interactionbetween Ese-2 and cytokeratin 18(K18)intron 1 was characterized in lung epithelial cells.Reporter gene assays showedEse-2 was able to upregulate K18 intron 1 enhanced reporter gene expression by approximately 2-fold.We found thatfull length Ese-2 did not bind DNA strongly,therefore truncated versions of the protein,containing the ETS domain orPointed domain,were created and tested in electrophoresis mobility shift assays.Multiple interactions between the ETSdomain and putative DNA binding sites within K18 intron 1 were observed,which led to the determination of a possibleEse-2 DNA binding consensus sequence.These experiments suggest that Ese-2 could play a role in the regulation ofK18 expression in lung epithelial cells.展开更多
基金This work was supported by operating grants from the Canadian Institutes of Health Researchthe Canadian Cystic Fibrosis Foundation(to J.H.)the Foundation Fighting Blindness—Canada(to J.H.).
文摘The role of Ese-2,an Ets family transcription factor,in gene regulation is not known.In this study,the interactionbetween Ese-2 and cytokeratin 18(K18)intron 1 was characterized in lung epithelial cells.Reporter gene assays showedEse-2 was able to upregulate K18 intron 1 enhanced reporter gene expression by approximately 2-fold.We found thatfull length Ese-2 did not bind DNA strongly,therefore truncated versions of the protein,containing the ETS domain orPointed domain,were created and tested in electrophoresis mobility shift assays.Multiple interactions between the ETSdomain and putative DNA binding sites within K18 intron 1 were observed,which led to the determination of a possibleEse-2 DNA binding consensus sequence.These experiments suggest that Ese-2 could play a role in the regulation ofK18 expression in lung epithelial cells.
