Here we report a new method to detect DNA point mutations.The method is based on the formation and deformation of double-stranded DNA(dsDNA)membranes on a gold surface.It can encage reporter molecules between the gold...Here we report a new method to detect DNA point mutations.The method is based on the formation and deformation of double-stranded DNA(dsDNA)membranes on a gold surface.It can encage reporter molecules between the gold surface and the double-stranded DNA or keep them away from the gold surface.In these systems,Fe(CN)63−was used as the reporter.As the temperature increases,a sharp electrochemical signal change in the melting curve of wild-type dsDNA appears.At a special temperature,the method gives 100:1 selectivity for the perfect complement and single base mutation target.Thus,the system provides a simple and sensitive method to detect DNA point mutations without labeling targets.展开更多
基金supported by Hi-Tech Research and Developmont Program of China (No.2002AA221011)。
文摘Here we report a new method to detect DNA point mutations.The method is based on the formation and deformation of double-stranded DNA(dsDNA)membranes on a gold surface.It can encage reporter molecules between the gold surface and the double-stranded DNA or keep them away from the gold surface.In these systems,Fe(CN)63−was used as the reporter.As the temperature increases,a sharp electrochemical signal change in the melting curve of wild-type dsDNA appears.At a special temperature,the method gives 100:1 selectivity for the perfect complement and single base mutation target.Thus,the system provides a simple and sensitive method to detect DNA point mutations without labeling targets.
