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A simple and efficient method for the preparation of live leukocytes from peripheral blood using the LeukoCatch<sup>TM</sup>system
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作者 Ayumi Okamoto Kosuke Torigata +7 位作者 Minami A. Sakurai Daisuke Okuzaki Hodaka Fujii Toshinari Ohmine Daisaku Miura Shoichi Kimura Norikazu Yabuta Hiroshi Nojima 《Advances in Bioscience and Biotechnology》 2012年第5期630-642,共13页
Leukocytes from peripheral blood (PB) are of great value for diagnosis as well as basic and clinical research. However, no easy, centrifugation-free method is available for the isolation of live leukocytes from blood.... Leukocytes from peripheral blood (PB) are of great value for diagnosis as well as basic and clinical research. However, no easy, centrifugation-free method is available for the isolation of live leukocytes from blood. We here develop a simple and quick method for the purification of viable leukocytes from whole blood using novel tools, named tLeukoCatch (tip-type) or sLeukoCatch (syringe-type), which is equipped with three Pall filter layers and captures leukocytes but not red blood cells (RBCs) in whole blood. Indeed, we showed that several million leukocytes per mL (~35% of the recovery rate) were captured and eluted from whole blood. The number of contaminant RBCs decreased from several million to several thousand. When mouse blood was hemolysed, almost all of the lysed RBC fragments were removed by passage through sLeukoCatch. Optical microscopic observation confirmed that the recovered leukocytes were sufficiently healthy to respond to growth stimuli. Efficient leukocyte recovery was also confirmed for hemolysed human blood. These results suggest that the LeukoCatchTM system is useful for bedside diagnosis and basic research with blood samples. 展开更多
关键词 Novel Tool LEUKOCYTE Purification Centrifugation-Free Diagnosis PBMC
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Association of ITPA polymorphism with outcomes of peginterferon-α plus ribavirin combination therapy 被引量:1
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作者 Tatsuya Fujino Yoko Aoyagi +12 位作者 Mariko Takahashi Ryoko Yada Naoko Yamamoto Yuki Ohishi Akihiko Nishiura Motoyuki Kohjima Tsuyoshi Yoshimoto Kunitaka Fukuizumi Manabu Nakashima Masaki Kato Kazuhiro Kotoh Makoto Nakamuta Munechika Enjoji 《World Journal of Gastrointestinal Pharmacology and Therapeutics》 CAS 2013年第3期54-60,共7页
AIM:To analyzed the association between inosine triphosphatase(ITPA)(rs1127354) genotypes and sustained virological response(SVR) rates in peginterferon(Peg-IFN)α + ribavirin(RBV) treatment.METHODS:Patients who under... AIM:To analyzed the association between inosine triphosphatase(ITPA)(rs1127354) genotypes and sustained virological response(SVR) rates in peginterferon(Peg-IFN)α + ribavirin(RBV) treatment.METHODS:Patients who underwent Peg-IFNα + RBV combination therapy were enrolled(n = 120) and they had no history of other IFN-based treatments.Variation in hemoglobin levels during therapy,cumulative reduction of RBV dose,frequency of treatment withdrawal,and SVR rates were investigated in each ITPA genotype.RESULTS:In patients with ITPA CC genotype,hemoglobin decline was significantly greater and the percentage of patients in whom total RBV dose was < 60% of standard and/or treatment was withdrawn was significantly higher compared with CA/AA genotype.However,SVR rates were equivalent between CC and CA/AA genotypes,and within a subset of patients with Interleukin 28B(IL28B)(rs8099917) TT genotype,SVR rates tended to be higher in patients with ITPA CC genotype,although the difference was not significant.CONCLUSION:ITPA CC genotype was a disadvantageous factor for Peg-IFNα + RBV treatment in relation to completion rates and RBV dose.However,CC genotype was not inferior to CA/AA genotype for SVR rates.When full-length treatment is accomplished,it is plausible that more SVR is achieved in patients with ITPA CC variant,especially in a background of IL28B TT genotype. 展开更多
关键词 Chronic HEPATITIS C INTERLEUKIN 28B INOSINE triphosphatase PEGINTERFERON RIBAVIRIN
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