Two novel xanthones, 1,6-dihydroxy-4,5-dimethoxyxanthone （1） and 1,5,6-trihydroxy-7,8-di（3-methyl-2-butenyl）-6＇,6＇-dimethylpyrano（2＇,3＇：3,4）xanthone （2） were isolated from the bark of Garcinia xanthochymu...Two novel xanthones, 1,6-dihydroxy-4,5-dimethoxyxanthone （1） and 1,5,6-trihydroxy-7,8-di（3-methyl-2-butenyl）-6＇,6＇-dimethylpyrano（2＇,3＇：3,4）xanthone （2） were isolated from the bark of Garcinia xanthochymus by normal phase and reverse phase silica gel column chromatography. Their structures were elucidated by spectroscopic methods, especially 2D-NMR techniques.展开更多
On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical co...On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical composition,pharmacological action and toxicological action,so as to provide a reference basis for further research and further development and utilization of Mongolian medicine.展开更多
The aim of the present study was to investigate the protective effects and underlying mechanisms of Garcinia xanthochymus, a perennial medicinal plant native to Yunnan, China, against H2 O2-induced oxidative damage in...The aim of the present study was to investigate the protective effects and underlying mechanisms of Garcinia xanthochymus, a perennial medicinal plant native to Yunnan, China, against H2 O2-induced oxidative damage in rat pheochromacytoma PC12 cells. Preincubation of PC12 cells with fruit Et OAc fraction(fruit-EFr., 12.5–50 μmol·L^(-1)) of G. xanthochymus for 24 h prior to H_2O_2 exposure markedly improved cell viability and increased the activities of antioxidant enzymes(superoxide dismutase, catalase, and heme oxygenase-1 [HO-1]), prevented lactate dehydrogenase release and lipid peroxidation malondialdehyde production, attenuated the decrease of matrix metalloproteinases(MMP), and scavenged reactive oxygen species(ROS). Fruit-EFr. also reduced BAX and cytochrome C expression and improved BCL-2 expression, thereby decreasing the ratio of BAX to BCL-2. Fruit-EFr. activated the nuclear translocation of NRF2 to increase HO-1 and induced the phosphorylation of AKT. Its cytoprotective effect was abolished by LY294002, a specific inhibitor of PI3 K. Taken together, the above findings suggested that fruit-EFr.of G. xanthochymus could enhance cellular antioxidant defense capacity, at least in part, through upregulating HO-1 expression and activating the PI3 K/AKT pathway and that it could suppress H_2O_2-induced oxidative damage via PI3 K/AKT and NRF2/HO-1 signaling pathways.展开更多
基金the National Natural Science Foundation of China （No. 30670215）
文摘Two novel xanthones, 1,6-dihydroxy-4,5-dimethoxyxanthone （1） and 1,5,6-trihydroxy-7,8-di（3-methyl-2-butenyl）-6＇,6＇-dimethylpyrano（2＇,3＇：3,4）xanthone （2） were isolated from the bark of Garcinia xanthochymus by normal phase and reverse phase silica gel column chromatography. Their structures were elucidated by spectroscopic methods, especially 2D-NMR techniques.
基金National Natural Science Foundation of China(81560702)Joint Research Project of Inner Mongolia Minzu University and Mongolian Academy of Sciences(NMDGJ0014)。
文摘On the basis of research on the origin of Mongolian medicine Veratrum nigrum L.,this paper summarizes the research situation of Mongolian medicine Veratrum nigrum L.from the aspects of clinical application,chemical composition,pharmacological action and toxicological action,so as to provide a reference basis for further research and further development and utilization of Mongolian medicine.
基金supported by the National Natural Science Foundation of China(No.31370379)the National Natural Science Foundation Youth Project Financing(No.81201610)+1 种基金State Ethnic Affairs Commission Research Project(No.CMZY13012)Universities of Hubei Province Outstanding Youth Scientific Innovation Team Plan(No.T201220)
文摘The aim of the present study was to investigate the protective effects and underlying mechanisms of Garcinia xanthochymus, a perennial medicinal plant native to Yunnan, China, against H2 O2-induced oxidative damage in rat pheochromacytoma PC12 cells. Preincubation of PC12 cells with fruit Et OAc fraction(fruit-EFr., 12.5–50 μmol·L^(-1)) of G. xanthochymus for 24 h prior to H_2O_2 exposure markedly improved cell viability and increased the activities of antioxidant enzymes(superoxide dismutase, catalase, and heme oxygenase-1 [HO-1]), prevented lactate dehydrogenase release and lipid peroxidation malondialdehyde production, attenuated the decrease of matrix metalloproteinases(MMP), and scavenged reactive oxygen species(ROS). Fruit-EFr. also reduced BAX and cytochrome C expression and improved BCL-2 expression, thereby decreasing the ratio of BAX to BCL-2. Fruit-EFr. activated the nuclear translocation of NRF2 to increase HO-1 and induced the phosphorylation of AKT. Its cytoprotective effect was abolished by LY294002, a specific inhibitor of PI3 K. Taken together, the above findings suggested that fruit-EFr.of G. xanthochymus could enhance cellular antioxidant defense capacity, at least in part, through upregulating HO-1 expression and activating the PI3 K/AKT pathway and that it could suppress H_2O_2-induced oxidative damage via PI3 K/AKT and NRF2/HO-1 signaling pathways.
