Diversity and Distribution of Host Animal Species of Hantavirus and Risk to Human Health in Jiuhua Mountain Area,China

Objective To investigate the diversity and the distribution of host animal species of hantavirus and the effect on human health in Jiuhua Mountain area,China.Methods The host animal species of hantavirus was surveyed by using the trap method and the species diversity was evaluated by using the Simpson,Shannon-Weaner,and Pielou indices.Hantavirus antigens or antibodies in lung and blood samples of all the captured host animals were detected by direct or indirect immunofluorescence.Results Nine animal species of hantavirus were distributed in the forest ecosystem of Jiuhua Mountain.Of these,Niviventer confucianus and Apodemus agrarius were predominant,and N.confucianus,Rattus norvegicus,and Mus musculus had relatively large niche breadth index values.The host animals in the eastern and western mountain regions shared similar biodiversity index characteristics,predominant species,and species structures.Hantavirus was detected in 5 host animal species in Jiuhua Mountain area,the carriage rate of hantavirus was 6.03%.The average density of host animals in forest areas of the mountainous area was only 2.20%,and the virus infection rate in the healthy population was 2.33%.Conclusion The circulation of hantavirus was low in the forest areas of Jiuhua Mountain and did not pose a threat to human health.

Synthesis, Characterization, and Antifungal Evaluation of Thiolactomycin Derivatives

5-Substituted benzylidene 3-acylthiotetronic acids are antifungal.A series of 3-acylthiotetronic acid derivatives with varying substitutions at the 5-position were designed,synthesized,and characterized,based on the binding pose of 3-acyl thiolactone with the protein C171Q KasA.Fungicidal activities of these compounds were screened against Valsa Mali,Curvularia lunata,Fusarium graminearum,and Fusarium oxysporum f.sp.lycopersici.Most target compounds exhibited excellent fungicidal activities against target fungi at the concentration of 50μg·mL-1.Compounds 11c and 11i displayed the highest activity with a broad spectrum.The median effective concentration(EC50)values of 11c and 11i were 1.9–10.7 and 3.1–7.8μg·mL-1,respectively,against the tested fungi,while the EC50 values of the fungicides azoxystrobin,carbendazim,and fluopyram were respectively 0.30,4.22,and>50μg·mL-1 against V.Mali;6.7,41.7,and 0.18μg·mL-1 against C.lunata;22.4,0.42,and 0.43μg·mL-1 against F.graminearum;and 4.3,0.12,and>50μg·mL-1 against F.oxysporum f.sp.lycopersici.The structures and activities of the target compounds against C.lunata were analyzed to obtain a statistically significant comparative molecular field analysis(CoMFA)model with high prediction abilities(q2=0.9816,r2=0.8060),and its reliability was verified.The different substituents on the benzylidene at the 5-position had significant effects on the activity,while the introduction of a halogen atom at the benzene ring of benzylidene was able to improve the activity against the tested fungi.

Restoring the plant productivity of heavy metal-contaminated soil using phosphate sludge,marble waste,and beneficial microorganisms

Assisted natural remediation(ANR)has been highlighted as a promising,less expensive,and environmentally friendly solution to remediate soil contaminated with heavy metals.We tested the effects of three amendments(10%compost,C;5 or 15%phosphate sludge,PS5 and PS15;and 5 or 15%marble waste,MW5 and MW15)in combination with microorganism inoculation(rhizobacteria consortium alone,mycorrhizae alone,and the two incombination)on alfalfa in contaminated soil.Plant concentrations of Zn,Cu,and Pb were measured,along with proline and malondialdehyde production.The microbiological and physicochemical properties of the mining soil were evaluated.Application of the amendments allowed germination and promoted growth.Inoculation with the rhizobacteria consortium and/or mycorrhizae stimulated plant growth.PS and MW stimulated the production of proline.Inoculation of alfalfa with the rhizobacteria-mycorrhizae mixture and the application of MW allowed the safe cultivation of the legume,as shown by the low concentrations of metals in plant shoots.Zn and Pb concentrations were below the limits recommended for animal grazing and accumulated essentially in roots.Soil analyses showed the positive effect of the amendments on the soil physicochemical properties.All treatments increased soil p H(around 7),total organic carbon,and assimilable phosphorus content.Notably,an important decrease in soluble heavy metals concentrations was observed.Overall,our findings revealed that the applied treatments reduced the risk of metal-polluted soils limiting plant growth.The ANR has great potential for success in the restoration of polymetallic and acidic mining soils using the interaction between alfalfa,microorganisms,and organomineral amendments.

Design, Synthesis and Application of a Robust d_(3)-Methyithiolating Reagent

A deuterated methylthiolating reagent with a broad spectrum,designated as DMTp,has been ingeniously formulated.This new reagent can be produced with high efficiency through the simple combination of commercially available methyl 3-thiopropanoate and CD_(3)l.This novel SCD_(3) reagent exhibits excellent reactivity with electrophiles such as alkyl iodides,benzyl chlorides,aryl halides(l,Br)and aryl triflates,resulting in high levels of deuterium content(99%D).Representative deuterated methylthiolated biomolecules,such as Sulfoxaflor,havebeen synthesizedforuse in pharmaceutical discoveryand development.