Introduction: Peanut pastes are food products resulting from artisanal or industrial processing, used in cooking in Africa in general and in Central African Republic in particular. These peanut pastes are often contam...Introduction: Peanut pastes are food products resulting from artisanal or industrial processing, used in cooking in Africa in general and in Central African Republic in particular. These peanut pastes are often contaminated by molds and filamentous fungi involved in the degradation of hygienic and organoleptic or even toxicological quality. This study aims to determine the epidemiological profile of molds contaminating peanut pastes sold on the Central African market. Methodology: This was a cross-sectional study carried out from June to September 2023. Samples of peanut pastes sold on Central African market were taken and analyzed at the National Laboratory of Clinical Biology and Public Health using the conventional microbiology method according to ISO 7954 standards. The data obtained were collected in the ODK 2023.3.1 application and analyzed with the Epi Info 7 software. A multivariate analysis by logistic regression, Ficher’s exact test, and chi<sup>2</sup> at the 5% threshold (p Penicillium sp.;11.25% of Mucor sp.;10.63% of Aspergillus terrei;3.13% of Aspergillus niger;1.25% of Aspergillus medullans;28.13% of Aspergillus flavus;2.50% of Aspergillus fumigatus. Peanut pastes stored beyond three days were more contaminated (94.19%). Conclusion: The results of this study made it possible to highlight strains of mold that impact the hygienic and organoleptic quality of peanut pastes sold at the Central African market. Most of the isolated strains were the Aspergillus flavus species which is recognized by its toxigenic effects. This species is much more incriminated in the contamination of foodstuffs with the production of the toxin which causes underlying pulmonary pathologies in humans.展开更多
This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated Decembe...This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated December 17, 2001 and n˚407 dated October 3, 2008 respectively. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazolyl-2-yl-2,5-diphenyltetrazolium bromide (MTT) test. No significant toxicity was observed after 14 and 28 days, although a dose-dependent decrease in creatinemia was observed in male rats (the recipe to be used to moderate creatinine levels). Cytotoxicity was without effect on NCM 365 cells. The results obtained justify the use of the recipe in traditional medicine.展开更多
The antitumor effects of conventional drug carriers are often attenuated and limited in application by biological barriers associated with tumor heterogeneity and resistance brought about by low tumor immunogenicity.W...The antitumor effects of conventional drug carriers are often attenuated and limited in application by biological barriers associated with tumor heterogeneity and resistance brought about by low tumor immunogenicity.With the rapid development of nanotechnology,naturally derived bioactive materials,and live cell carriers,a promising strategy emerges for targeting the tumor microenvironment(TME)for precision cancer therapy.The unique injury-targeting properties of platelets can significantly extend functional activity,which cannot be achieved with conventional nanocarriers.In this review,three strategies based on platelet-engineered materials are systematically described,namely nanoparticles for platelet membrane camouflage,engineered activated platelets,and targeted-platelets nanosystems.Platelet-based nanomaterials can effectively coordinate local and distant tumor-host crosstalk with controlled active tumor site recognition and killing effects due to the presence of specific membrane proteins on the surface and the self-secretion of a large number of particles.Further advances in platelets for effectively overcoming biological barriers and reducing immune resistance in cancer immunotherapy applications will be discussed in future clinical practice.This review provides an overview of recent research advances in platelet-based bioactive material-directed immunotherapy and chemotherapy to inform future antitumor combination therapies.展开更多
Androgenetic alopecia(AGA)is a chronic and progressive form of hair loss characterized by vascular degeneration in the perifollicular microenvironment,leading to cell apoptosis and eventual loss of hair follicles(HFs)...Androgenetic alopecia(AGA)is a chronic and progressive form of hair loss characterized by vascular degeneration in the perifollicular microenvironment,leading to cell apoptosis and eventual loss of hair follicles(HFs).Traditional therapeutic formulations,such as Minoxidil(MXD)tincture,have limitations in reshaping the perifollicular microenvironment and exhibit limited effectiveness.Here,we report a multi-synergistic therapeutic platform for high-performance hair regeneration therapy.The platform combines microneedle(MN)patches loaded with MXD-encapsulated nanostructured lipid carriers(MXD-NLC-MNs)and cold atmospheric plasma(CAP).The MNs’mechanical strength enables efficient transdermal delivery of MXD to the targeted dermal papilla cells,promoting cell proliferation.Furthermore,in collaboration with MXD,the mechanical stimulation exerted by MN application synergistically upregulates the expression of vascular endothelial growth factor,leading to neoangiogenesis.Meanwhile,the transient microchannels in the skin created by MNs facilitate the transdermal delivery of CAPgenerated nitric oxide(NO)to the sites of HF lesions,whereby the synergistic interaction between MXD and NO boosts perifollicular vasodilation.Consequently,the perifollicular microenvironment can be effectively reshaped to accelerate hair regeneration in AGA murine models.This multi-synergistic combination therapy strategy would hold great promise for effectively treating AGA and promoting hair regrowth.展开更多
Despite the rapid development of probes for targeting single organelle, the construction of robust dual-organelle targeting probes with multicolor emission was rarely reported. Herein, two dual-emissive aggregation-in...Despite the rapid development of probes for targeting single organelle, the construction of robust dual-organelle targeting probes with multicolor emission was rarely reported. Herein, two dual-emissive aggregation-induced emission luminogens(AIEgens)with donor-π-acceptor structures were designed and synthesized, namely QT-1 and QF-2. Both two AIEgens exhibited excitation wavelength-dependence defying the Kasha's rule, and could stain lipid droplets(LDs) and mitochondria in blue and red fluorescence, respectively. Moreover, thanks to the near-infrared emission and abundant reactive oxygen species(ROS) generation efficiency of QT-1, it was chosen as a photodynamic therapy agent to selectively kill cancer cells from normal cells. Upon light irradiation, an obvious decrease of mitochondrial membrane potential(MMP) and serious change of mitochondrial shape in cells were observed, which corresponded to the efficient inhibition of tumor growth in vivo. This work afforded a promising strategy for the construction of multicolor emission by tuning anti-Kasha behaviors and expanding their application in dualorganelle targeting-based phototheranostics.展开更多
High mobility group box 1(HMGB1)is an evolutionarily conserved non-histone chromatin-binding protein.During infection or injury,activated immune cells and damaged cells release HMGB1 into the extracellular space,where...High mobility group box 1(HMGB1)is an evolutionarily conserved non-histone chromatin-binding protein.During infection or injury,activated immune cells and damaged cells release HMGB1 into the extracellular space,where HMGB1 functions as a proinflammatory mediator and contributes importantly to the pathogenesis of inflammatory diseases.Recent studies reveal that infl ammasomes,intracellular protein complexes,critically regulate HMGB1 release from activated immune cells in response to a variety of exogenous and endogenous danger signals.Double stranded RNA dependent kinase(PKR),an intracellular danger-sensing molecule,physically interacts with inflammasome components and is important for infl ammasome activation and HMGB1 release.Together,these studies not only unravel novel mechanisms of HMGB1 release during infl ammation,but also provide potential therapeutic targets to treat HMGB1-related infl ammatory diseases.展开更多
Amelogenin isoforms constitute the predominant component in the enamel matrix and each amelogenin isoform executes unique role in the enamel biomineralization process. Enamel matrix derivative enriching amelogenin iso...Amelogenin isoforms constitute the predominant component in the enamel matrix and each amelogenin isoform executes unique role in the enamel biomineralization process. Enamel matrix derivative enriching amelogenin isoforms have also hioactive property for tissue regeneration. Despite the development of recombinant protein technology that has greatly forwarded the understanding ofamelogenin properties, substantial evidences have revealed biochemical and functional difference between natural amelogenins and their recombinant form. To facilitate the study of enamel formation mechanism, more facile methodology to purify multiple natural amelogenin isoforms is pursued. Here we developed an effective one-shot method via reverse phase high-performance liquid chromatography (RP-HPLC) to purify various amelogenin isoforms from pig-derived amelogenin complex. A thorough process of chromatographic condition establishment including sample analysis on analytical scale and chromatographic condition design on preparative scale was described. Three representative amelogenin isoforms (TRAP, P148, P173) were isolated in one step and their purity was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and high resolution mass spectrometry.展开更多
In this research, polypyrrole nanocone arrays doped with β-Naphthalene sulphonic acid (PPy-NSA) were built. This film was expected to control protein adsorption and bacterial adhesion by potential-induced reversibl...In this research, polypyrrole nanocone arrays doped with β-Naphthalene sulphonic acid (PPy-NSA) were built. This film was expected to control protein adsorption and bacterial adhesion by potential-induced reversibly redox. The scanning Kelvin probe microscopy (SKPM) and surface contact angles (SCA) tests suggested that the surface potential and wettability of PPy-NSA nanocone arrays could be controlled by simply controlling its redox property via applying potential. The controllable surface potential and wettability in return controlled the adsorption of protein and adhesion of bacteria. The proposed material might find application in the preparation of smart biomaterial surfaces that can regulate proteins and bacterial adhesion by a simple potential switching.展开更多
To improve the osteointegration property of biomedical titanium,nano-architectured electroactive coating was synthesized through the electrochemical polymerization of dopamine and pyrrole.The highly binding affinity o...To improve the osteointegration property of biomedical titanium,nano-architectured electroactive coating was synthesized through the electrochemical polymerization of dopamine and pyrrole.The highly binding affinity of Ca2t to the catechol moiety of doped dopamine enabled efficient interaction between polypyrrole/polydopamine nanowires and mineral ions.The results indicate that the PPy/PDA nanowires preserved its efficient electro-activity and accelerated the hydroxyapatite deposition in a simulated body fluid.The PPy/PDA nanowires coating could be applied to promote the osteointegration of titanium implant.展开更多
Yeast cells have controllable biosorption on metallic ions during metabolism.However,few studies were dedicated to using yeast-regulated biomimetic mineralization process to control the strontium-doped positions in ca...Yeast cells have controllable biosorption on metallic ions during metabolism.However,few studies were dedicated to using yeast-regulated biomimetic mineralization process to control the strontium-doped positions in calcium phosphate microcapsules.In this study,the yeast cells were allowed to pre-adsorb strontium ions metabolically and then served as sacrificing template for the precipitation and calcination of mineral shell.The pre-adsorption enabled the microorganism to enrich of strontium ions into the inner part of the microcapsules,which ensured a slow-release profile of the trace element from the microcapsule.The co-culture with human marrow stromal cells showed that gene expressions of alkaline phosphatase and Collagen-I were promoted.The promotion of osteogenic differentiation was further confirmed in the 3D culture of cell-material complexes.The strategy using living microorganism as‘smart doping apparatus’to control incorporation of trace element into calcium phosphate paved a pathway to new functional materials for hard tissue regeneration.展开更多
Catheters navigating through complex vessels,such as sharp turns or multiple U-turns,remain challenging for vascular embolization.Here,we propose a novel multistage vascular embolization strategy for hard-to-reach ves...Catheters navigating through complex vessels,such as sharp turns or multiple U-turns,remain challenging for vascular embolization.Here,we propose a novel multistage vascular embolization strategy for hard-to-reach vessels that releases untethered swimming shape-memory magnetic microrobots(SMMs)from the prior catheter to the vessel bifurcation.SMMs,made of organo-gel with magnetic particles,ensure biocompatibility,radiopacity,thrombosis,and fast thermal and magnetic responses.An SMM is initially a linear shape with a 0.5-mm diameter at 20°C inserted in a catheter.It transforms into a predetermined helix within 2 s at 38°C blood temperature after being pushed out of the catheter into the blood.SMMs enable agile swimming in confined and tortuous vessels and can swim upstream using helical propulsion with rotating magnetic fields.Moreover,we validated this multistage vascular embolization in living rabbits,completing 100-cm travel and renal artery embolization in 2 min.After 4 weeks,the SMMs maintained the embolic position,and the kidney volume decreased by 36%.展开更多
文摘Introduction: Peanut pastes are food products resulting from artisanal or industrial processing, used in cooking in Africa in general and in Central African Republic in particular. These peanut pastes are often contaminated by molds and filamentous fungi involved in the degradation of hygienic and organoleptic or even toxicological quality. This study aims to determine the epidemiological profile of molds contaminating peanut pastes sold on the Central African market. Methodology: This was a cross-sectional study carried out from June to September 2023. Samples of peanut pastes sold on Central African market were taken and analyzed at the National Laboratory of Clinical Biology and Public Health using the conventional microbiology method according to ISO 7954 standards. The data obtained were collected in the ODK 2023.3.1 application and analyzed with the Epi Info 7 software. A multivariate analysis by logistic regression, Ficher’s exact test, and chi<sup>2</sup> at the 5% threshold (p Penicillium sp.;11.25% of Mucor sp.;10.63% of Aspergillus terrei;3.13% of Aspergillus niger;1.25% of Aspergillus medullans;28.13% of Aspergillus flavus;2.50% of Aspergillus fumigatus. Peanut pastes stored beyond three days were more contaminated (94.19%). Conclusion: The results of this study made it possible to highlight strains of mold that impact the hygienic and organoleptic quality of peanut pastes sold at the Central African market. Most of the isolated strains were the Aspergillus flavus species which is recognized by its toxigenic effects. This species is much more incriminated in the contamination of foodstuffs with the production of the toxin which causes underlying pulmonary pathologies in humans.
文摘This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated December 17, 2001 and n˚407 dated October 3, 2008 respectively. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazolyl-2-yl-2,5-diphenyltetrazolium bromide (MTT) test. No significant toxicity was observed after 14 and 28 days, although a dose-dependent decrease in creatinemia was observed in male rats (the recipe to be used to moderate creatinine levels). Cytotoxicity was without effect on NCM 365 cells. The results obtained justify the use of the recipe in traditional medicine.
基金supported by the National Key Research and Development Program of China(Nos.2022YFC2402400 and 2023YFA0915400)Guangdong Provincial Key Area R&D Program(No.2020B1111540001)+5 种基金the Natural Science Foundation of Guangdong Province(Nos.2024A1515010825,2024A1515030063,2022A1515010780,and 2022A1515011337)Guangdong Province Universities and Colleges Characteristic Innovation(No.2021KTSCX036)Traditional Chinese Medicine Research Project of Guangdong Province Traditional Chinese Medicine Bureau(No.20221206)Shenzhen Medical Research Fund(No.A2303057),Discipline Construction Project of Guangdong Medical University(No.4SG24015G)Construction Project of Nano Technology and Application Engineering Research Center of Guangdong Medical University(No.4SG24179G)Funds for PHD researchers of Guangdong Medical University in 2024.
文摘The antitumor effects of conventional drug carriers are often attenuated and limited in application by biological barriers associated with tumor heterogeneity and resistance brought about by low tumor immunogenicity.With the rapid development of nanotechnology,naturally derived bioactive materials,and live cell carriers,a promising strategy emerges for targeting the tumor microenvironment(TME)for precision cancer therapy.The unique injury-targeting properties of platelets can significantly extend functional activity,which cannot be achieved with conventional nanocarriers.In this review,three strategies based on platelet-engineered materials are systematically described,namely nanoparticles for platelet membrane camouflage,engineered activated platelets,and targeted-platelets nanosystems.Platelet-based nanomaterials can effectively coordinate local and distant tumor-host crosstalk with controlled active tumor site recognition and killing effects due to the presence of specific membrane proteins on the surface and the self-secretion of a large number of particles.Further advances in platelets for effectively overcoming biological barriers and reducing immune resistance in cancer immunotherapy applications will be discussed in future clinical practice.This review provides an overview of recent research advances in platelet-based bioactive material-directed immunotherapy and chemotherapy to inform future antitumor combination therapies.
基金supported by the National Key Research and Development Program of China(Nos.2022YFE0126000(Z.T.C.),and 2020YFA0210800(Z.W.C.))the National Natural Science Foundation of China(Nos.22277011(Z.W.C.),and 22107019(Z.W.C.))+1 种基金the Major Project of Science and Technology of Fujian Province(No.2020HZ06006(Z.W.C.))the Guangdong Basic and Applied Basic Research Foundation(No.2022A1515011129((Z.T.C.)).
文摘Androgenetic alopecia(AGA)is a chronic and progressive form of hair loss characterized by vascular degeneration in the perifollicular microenvironment,leading to cell apoptosis and eventual loss of hair follicles(HFs).Traditional therapeutic formulations,such as Minoxidil(MXD)tincture,have limitations in reshaping the perifollicular microenvironment and exhibit limited effectiveness.Here,we report a multi-synergistic therapeutic platform for high-performance hair regeneration therapy.The platform combines microneedle(MN)patches loaded with MXD-encapsulated nanostructured lipid carriers(MXD-NLC-MNs)and cold atmospheric plasma(CAP).The MNs’mechanical strength enables efficient transdermal delivery of MXD to the targeted dermal papilla cells,promoting cell proliferation.Furthermore,in collaboration with MXD,the mechanical stimulation exerted by MN application synergistically upregulates the expression of vascular endothelial growth factor,leading to neoangiogenesis.Meanwhile,the transient microchannels in the skin created by MNs facilitate the transdermal delivery of CAPgenerated nitric oxide(NO)to the sites of HF lesions,whereby the synergistic interaction between MXD and NO boosts perifollicular vasodilation.Consequently,the perifollicular microenvironment can be effectively reshaped to accelerate hair regeneration in AGA murine models.This multi-synergistic combination therapy strategy would hold great promise for effectively treating AGA and promoting hair regrowth.
基金supported by the National Natural Science Foundation of China (52173152, 21805002)Guangdong Basic and Applied Basic Research Foundation (2020A1515110476)+7 种基金the Fund of the Rising Stars of Shaanxi Province (2021KJXX-48)the Shenzhen Science and Technology Program (KQTD20210811090115019)the Major Instrumentation Development Program of the Chinese Academy of Sciences(ZDKYYQ20220008)Shenzhen Basic Research (key project)(China)(JCYJ20210324120011030)the Scientific and Technological Innovation Team of Shaanxi Province (2022TD-36)the National Key R&D Programs(China)(2021YFA0910001)Shaanxi Fundamental Science Research Project for Chemistry&Biology (22JHQ078)the Scientific Research Program Funded by Shaanxi Provincial Education Department (22JK0247)。
文摘Despite the rapid development of probes for targeting single organelle, the construction of robust dual-organelle targeting probes with multicolor emission was rarely reported. Herein, two dual-emissive aggregation-induced emission luminogens(AIEgens)with donor-π-acceptor structures were designed and synthesized, namely QT-1 and QF-2. Both two AIEgens exhibited excitation wavelength-dependence defying the Kasha's rule, and could stain lipid droplets(LDs) and mitochondria in blue and red fluorescence, respectively. Moreover, thanks to the near-infrared emission and abundant reactive oxygen species(ROS) generation efficiency of QT-1, it was chosen as a photodynamic therapy agent to selectively kill cancer cells from normal cells. Upon light irradiation, an obvious decrease of mitochondrial membrane potential(MMP) and serious change of mitochondrial shape in cells were observed, which corresponded to the efficient inhibition of tumor growth in vivo. This work afforded a promising strategy for the construction of multicolor emission by tuning anti-Kasha behaviors and expanding their application in dualorganelle targeting-based phototheranostics.
文摘High mobility group box 1(HMGB1)is an evolutionarily conserved non-histone chromatin-binding protein.During infection or injury,activated immune cells and damaged cells release HMGB1 into the extracellular space,where HMGB1 functions as a proinflammatory mediator and contributes importantly to the pathogenesis of inflammatory diseases.Recent studies reveal that infl ammasomes,intracellular protein complexes,critically regulate HMGB1 release from activated immune cells in response to a variety of exogenous and endogenous danger signals.Double stranded RNA dependent kinase(PKR),an intracellular danger-sensing molecule,physically interacts with inflammasome components and is important for infl ammasome activation and HMGB1 release.Together,these studies not only unravel novel mechanisms of HMGB1 release during infl ammation,but also provide potential therapeutic targets to treat HMGB1-related infl ammatory diseases.
基金financially supported by the National Natural Science Foundation of China (No. 51572087)the 111 Project (No. B13039)+1 种基金the Science and Technology Program of Guangdong Province (No. 2013B010403007)the Program for Changjiang Scholars and Innovative Research Team in University (IRT 0919)
文摘Amelogenin isoforms constitute the predominant component in the enamel matrix and each amelogenin isoform executes unique role in the enamel biomineralization process. Enamel matrix derivative enriching amelogenin isoforms have also hioactive property for tissue regeneration. Despite the development of recombinant protein technology that has greatly forwarded the understanding ofamelogenin properties, substantial evidences have revealed biochemical and functional difference between natural amelogenins and their recombinant form. To facilitate the study of enamel formation mechanism, more facile methodology to purify multiple natural amelogenin isoforms is pursued. Here we developed an effective one-shot method via reverse phase high-performance liquid chromatography (RP-HPLC) to purify various amelogenin isoforms from pig-derived amelogenin complex. A thorough process of chromatographic condition establishment including sample analysis on analytical scale and chromatographic condition design on preparative scale was described. Three representative amelogenin isoforms (TRAP, P148, P173) were isolated in one step and their purity was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and high resolution mass spectrometry.
基金the financial support of the National Basic Research Program of China (Grant No. 2012CB619100)the National High Technology Research and Development Program of China (863 Program, Grant No. 2015AA033502)+2 种基金the National Natural Science Foundation of China (Grant Nos. 51372087, 51072055 and 51232002)the Science and Technology Planning Project of Guangdong Province, China (Grant No. 2014A010105048)the State Key Laboratory for Mechanical Behavior of Materials, China (Grant No. 20141607)
文摘In this research, polypyrrole nanocone arrays doped with β-Naphthalene sulphonic acid (PPy-NSA) were built. This film was expected to control protein adsorption and bacterial adhesion by potential-induced reversibly redox. The scanning Kelvin probe microscopy (SKPM) and surface contact angles (SCA) tests suggested that the surface potential and wettability of PPy-NSA nanocone arrays could be controlled by simply controlling its redox property via applying potential. The controllable surface potential and wettability in return controlled the adsorption of protein and adhesion of bacteria. The proposed material might find application in the preparation of smart biomaterial surfaces that can regulate proteins and bacterial adhesion by a simple potential switching.
基金oundation of China(grant numbers 51372087,51541201)the National High Technology Research and Development Program of China(863 Program)(grant number 2015AA033502)+1 种基金the Science and technology program of Guangzhou(grant number 201707010200)the Natural Science Foundation of Guangdong Province(grant number 2016A030308014).
文摘To improve the osteointegration property of biomedical titanium,nano-architectured electroactive coating was synthesized through the electrochemical polymerization of dopamine and pyrrole.The highly binding affinity of Ca2t to the catechol moiety of doped dopamine enabled efficient interaction between polypyrrole/polydopamine nanowires and mineral ions.The results indicate that the PPy/PDA nanowires preserved its efficient electro-activity and accelerated the hydroxyapatite deposition in a simulated body fluid.The PPy/PDA nanowires coating could be applied to promote the osteointegration of titanium implant.
基金This work was supported by National Basic Research Program of China(2012CB619100)National Natural Science Foundation of China(51072056,51572087)+2 种基金the 111 Project(B13039)Key grant of Chinese Ministry of Education(313022)Program for Changjiang Scholars and Innovative Research Team in University(IRT 0919).
文摘Yeast cells have controllable biosorption on metallic ions during metabolism.However,few studies were dedicated to using yeast-regulated biomimetic mineralization process to control the strontium-doped positions in calcium phosphate microcapsules.In this study,the yeast cells were allowed to pre-adsorb strontium ions metabolically and then served as sacrificing template for the precipitation and calcination of mineral shell.The pre-adsorption enabled the microorganism to enrich of strontium ions into the inner part of the microcapsules,which ensured a slow-release profile of the trace element from the microcapsule.The co-culture with human marrow stromal cells showed that gene expressions of alkaline phosphatase and Collagen-I were promoted.The promotion of osteogenic differentiation was further confirmed in the 3D culture of cell-material complexes.The strategy using living microorganism as‘smart doping apparatus’to control incorporation of trace element into calcium phosphate paved a pathway to new functional materials for hard tissue regeneration.
基金the National Key Research and Development Project under Grant 2023YFB4705300in part by the National Natural Science Foundation of China under Grant U22A2064+3 种基金in part by the Youth Innovation Promotion Association of CASin part by the Shenzhen Science and Technology Program under Grant JCYJ20220818101611025in part by the Guangdong Basic and Applied Basic Research Foundation(2022B1515120010)and in part by SIAT-CUHK Joint Laboratory of Robotics and Intelligent Systems.
文摘Catheters navigating through complex vessels,such as sharp turns or multiple U-turns,remain challenging for vascular embolization.Here,we propose a novel multistage vascular embolization strategy for hard-to-reach vessels that releases untethered swimming shape-memory magnetic microrobots(SMMs)from the prior catheter to the vessel bifurcation.SMMs,made of organo-gel with magnetic particles,ensure biocompatibility,radiopacity,thrombosis,and fast thermal and magnetic responses.An SMM is initially a linear shape with a 0.5-mm diameter at 20°C inserted in a catheter.It transforms into a predetermined helix within 2 s at 38°C blood temperature after being pushed out of the catheter into the blood.SMMs enable agile swimming in confined and tortuous vessels and can swim upstream using helical propulsion with rotating magnetic fields.Moreover,we validated this multistage vascular embolization in living rabbits,completing 100-cm travel and renal artery embolization in 2 min.After 4 weeks,the SMMs maintained the embolic position,and the kidney volume decreased by 36%.