Objective To investigate whether intracellular amyloid β （iAβ） induces toxicity in wild type （WT） and APP/PS1 mice, a mouse model of Alzheimer＇s disease. Methods Different forms of Aβ aggregates were microinje...Objective To investigate whether intracellular amyloid β （iAβ） induces toxicity in wild type （WT） and APP/PS1 mice, a mouse model of Alzheimer＇s disease. Methods Different forms of Aβ aggregates were microinjected into cultured WT or APP/PS1 mouse hippocampal neurons. TUNEL staining was performed to examine neuronal cell death. Reactive oxidative species （ROS） were measured by MitoSOXTM Red mitochondrial superoxide indicator. Results Crude, monomer and protofibrilAβ induced more toxicity inAPP/PS1 neurons than in WT neurons. ROS are involved in mediating the vulnerability of APP/PS1 neurons to iAβ toxicity. Conclusion Oxidative stress may mediate cell death induced by iAβ in neurons.展开更多
Objective Curcumin is extracted from the turmeric plant （Curcuma longa Linn.） and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurospo...Objective Curcumin is extracted from the turmeric plant （Curcuma longa Linn.） and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine （STS） toxicity in cell culture. Methods Rat hippocampal neurons in primary culture were exposed to STS （20 μmol/L） and treated with curcumin （20 μmol/L）. Cell viability was tested by MTT assay and reactive oxygen species （ROS） were measured using the MitoSOX red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 （Csp3）, heat shock protein 70 （Hsp70） and Akt. Results The results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection. Conclusion Curcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS.展开更多
基金supported by grants from the National Basic Research Development Program of the Ministry of Science and Technology of China (2009CB941301)Roche Research Grant, Peking University President Research Grant and the Recruiting Research Grant, Ministry of Education of China
文摘Objective To investigate whether intracellular amyloid β （iAβ） induces toxicity in wild type （WT） and APP/PS1 mice, a mouse model of Alzheimer＇s disease. Methods Different forms of Aβ aggregates were microinjected into cultured WT or APP/PS1 mouse hippocampal neurons. TUNEL staining was performed to examine neuronal cell death. Reactive oxidative species （ROS） were measured by MitoSOXTM Red mitochondrial superoxide indicator. Results Crude, monomer and protofibrilAβ induced more toxicity inAPP/PS1 neurons than in WT neurons. ROS are involved in mediating the vulnerability of APP/PS1 neurons to iAβ toxicity. Conclusion Oxidative stress may mediate cell death induced by iAβ in neurons.
基金supported by the National Basic Research Development Program of China(2009CB941301)the National Natural Science Foundation of China (91132718, 30701138)+3 种基金the Peking University President Research Grantthe Ministry of Education Recruiting Research Grantthe Fundamental Research Funds for the Central Universities of China (1112KYZY41)the "985 Project" of Minzu University, China (MUC98504-14)
文摘Objective Curcumin is extracted from the turmeric plant （Curcuma longa Linn.） and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine （STS） toxicity in cell culture. Methods Rat hippocampal neurons in primary culture were exposed to STS （20 μmol/L） and treated with curcumin （20 μmol/L）. Cell viability was tested by MTT assay and reactive oxygen species （ROS） were measured using the MitoSOX red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 （Csp3）, heat shock protein 70 （Hsp70） and Akt. Results The results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection. Conclusion Curcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS.
