Objective： To identify the differential proteins in the serum with anti-asthma activity of acupuncture to pinpoint the substance basis of anti-asthma activity of acupuncture by proteomics method. Methods： Two-dimens...Objective： To identify the differential proteins in the serum with anti-asthma activity of acupuncture to pinpoint the substance basis of anti-asthma activity of acupuncture by proteomics method. Methods： Two-dimensional electrophoresis of protein was employed to analyze the differential proteins in the serum with anti-asthma activity of acupuncture, and identify the differential proteins with mass spectrum technique. Results： In adrenaleetomy （ADX）-asthma acupuncture group and asthma acupuncture group, two-dimensional eleetrophoresis showed that there was an obvious fuscous point （MW=44.1kD, PI=5.1）, which was 60S ribosomal protein L13, or hypothetical protein FLJ32191 analyzed by mass spectrum technique, different from other groups. In ADX-asthma acupuncture group, there were two obviously fuseous points （MW=39.8kD, PI=4.9 and MW=39.5kD, PI=5.0）, which were zinc finger protein 208, and thyroid hormone receptor-associated protein analyzed by mass spectrum technique, and two special proteins （MW=17.3kD, PI〈4.5 and 16.9kD, PI〈4.5）, which were Cyclophilin A and zinc finger protein 91 analyzed by mass spectrum technique, different from other groups. Conclusion： There are multi-target effects regulating the whole body in acupuncture treating asthma relating to immunoloregulation, gene expression, and protein svnthesization. The effects of acupuncture on the resnonse nroteins need further research.展开更多
基金National Basic Research Program of China (973 Program, 2009CB52290)National Natural Science Funds (30873299,30701123)Shanghai Leading Academic Discipline Project (S30304)
文摘Objective： To identify the differential proteins in the serum with anti-asthma activity of acupuncture to pinpoint the substance basis of anti-asthma activity of acupuncture by proteomics method. Methods： Two-dimensional electrophoresis of protein was employed to analyze the differential proteins in the serum with anti-asthma activity of acupuncture, and identify the differential proteins with mass spectrum technique. Results： In adrenaleetomy （ADX）-asthma acupuncture group and asthma acupuncture group, two-dimensional eleetrophoresis showed that there was an obvious fuscous point （MW=44.1kD, PI=5.1）, which was 60S ribosomal protein L13, or hypothetical protein FLJ32191 analyzed by mass spectrum technique, different from other groups. In ADX-asthma acupuncture group, there were two obviously fuseous points （MW=39.8kD, PI=4.9 and MW=39.5kD, PI=5.0）, which were zinc finger protein 208, and thyroid hormone receptor-associated protein analyzed by mass spectrum technique, and two special proteins （MW=17.3kD, PI〈4.5 and 16.9kD, PI〈4.5）, which were Cyclophilin A and zinc finger protein 91 analyzed by mass spectrum technique, different from other groups. Conclusion： There are multi-target effects regulating the whole body in acupuncture treating asthma relating to immunoloregulation, gene expression, and protein svnthesization. The effects of acupuncture on the resnonse nroteins need further research.
