Objective To study the relationship between polymorphism of cystathionine beta synthase （CBS） gene and development of congenital heart disease （CHD）. Methods One hundred and twenty-seven CHD case-parent triads wer...Objective To study the relationship between polymorphism of cystathionine beta synthase （CBS） gene and development of congenital heart disease （CHD）. Methods One hundred and twenty-seven CHD case-parent triads were recruited from Liaoning Province as patient group, and 129 healthy subjects without family history of birth defect were simultaneously recruited as control group together with their biological parents. For all subjects the polymorphism of CBS gene G919A locus was examined by PCR-ARMS method, Results The frequencies of three genotypes （w/w, w/m, and m/m） in control group were 27.2%, 58,4%, and 14.4%, respectively, with no significant difference in gender. A significant difference in the allele frequency was found between CHD patients and controls, the wild allele frequency was 67,9% in patients and 55.7% in controls CHD parents＇ genotype distribution was significantly different from that in controls. Further comparison of each type of CHD showed that genotype frequencies in several CHD subtypes were significantly different from those in their corresponding controls. The results of TDT analysis showed that no allele transmission disequilibrium existed in CHD nuclear families. Conclusions CBS gene G919A mutation is associated with the development of CHD, and the mutated allele may decrease the risk of CHD.展开更多
In this study,we investigated whether two brain regions,the bed nucleus of the stria terminalis(BNST)and the basolateral amygdala(BLA),affected male rats(Rattus norvigicus)ability to innately discriminate between a pr...In this study,we investigated whether two brain regions,the bed nucleus of the stria terminalis(BNST)and the basolateral amygdala(BLA),affected male rats(Rattus norvigicus)ability to innately discriminate between a predator odor(cat urine)and female rat urine.Muscimol,a GABAa receptor agonist,was bilaterally microinjected into either the BNST or BLA of rats through展开更多
基金This work was supported by Major State Basic Research Development Program of the People's Republic of China (No. 2001CB510305).
文摘Objective To study the relationship between polymorphism of cystathionine beta synthase （CBS） gene and development of congenital heart disease （CHD）. Methods One hundred and twenty-seven CHD case-parent triads were recruited from Liaoning Province as patient group, and 129 healthy subjects without family history of birth defect were simultaneously recruited as control group together with their biological parents. For all subjects the polymorphism of CBS gene G919A locus was examined by PCR-ARMS method, Results The frequencies of three genotypes （w/w, w/m, and m/m） in control group were 27.2%, 58,4%, and 14.4%, respectively, with no significant difference in gender. A significant difference in the allele frequency was found between CHD patients and controls, the wild allele frequency was 67,9% in patients and 55.7% in controls CHD parents＇ genotype distribution was significantly different from that in controls. Further comparison of each type of CHD showed that genotype frequencies in several CHD subtypes were significantly different from those in their corresponding controls. The results of TDT analysis showed that no allele transmission disequilibrium existed in CHD nuclear families. Conclusions CBS gene G919A mutation is associated with the development of CHD, and the mutated allele may decrease the risk of CHD.
文摘In this study,we investigated whether two brain regions,the bed nucleus of the stria terminalis(BNST)and the basolateral amygdala(BLA),affected male rats(Rattus norvigicus)ability to innately discriminate between a predator odor(cat urine)and female rat urine.Muscimol,a GABAa receptor agonist,was bilaterally microinjected into either the BNST or BLA of rats through
