High-throughput SNP detection microarrays were used here to explore the relationship between 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism C677T and the risk of gastric carcinoma among population...High-throughput SNP detection microarrays were used here to explore the relationship between 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism C677T and the risk of gastric carcinoma among population in Jiangsu region,by genotyping the specimens from 170 patients with gastric carcinoma and 140 age-and sex- matched control subjects.PCR products were spotted onto a 3-aminopropyltriethoxysilane coated glass slide to fabricate a microarray,then interrogated by hybridization with dual-color probes (Cy3,CyS) to determine the SNP genotype of each sample,and the relation between the genotypes and the risk of gastric carcinoma was analyzed.The frequencies of C677T genotype were CC(47.9%),CT(40%),CT(12.1%) in control group and CC(35.9%),CT(45.9%),TT(18.2%) in gastric carcinoma group,respectively.The individuals with 677CT+TT genotype group or 677TT had a 1.67-fold (95% CI:1.06-2.64) or 2.67-fold (95% CI:1.382-5.341) increased risk to develop gastric carcinoma compared with those having 677CC genotype. It was shown that the single nucleotide polymorphisms in the MTHFR gene are associated with the risk of gastric carcinoma in the Chinese population.展开更多
基金financially supported by the National Natural Science Foundation of China(No.60571032,60121101)the Trans-Century Training Programme Foundation for the Talents by the Ministry of Education of China(2003)+1 种基金the Doctoral Program of Higher Education for funding(20050286014)the Natural Science Foundation of Guangdong,Province,China(No.04008782).
文摘High-throughput SNP detection microarrays were used here to explore the relationship between 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism C677T and the risk of gastric carcinoma among population in Jiangsu region,by genotyping the specimens from 170 patients with gastric carcinoma and 140 age-and sex- matched control subjects.PCR products were spotted onto a 3-aminopropyltriethoxysilane coated glass slide to fabricate a microarray,then interrogated by hybridization with dual-color probes (Cy3,CyS) to determine the SNP genotype of each sample,and the relation between the genotypes and the risk of gastric carcinoma was analyzed.The frequencies of C677T genotype were CC(47.9%),CT(40%),CT(12.1%) in control group and CC(35.9%),CT(45.9%),TT(18.2%) in gastric carcinoma group,respectively.The individuals with 677CT+TT genotype group or 677TT had a 1.67-fold (95% CI:1.06-2.64) or 2.67-fold (95% CI:1.382-5.341) increased risk to develop gastric carcinoma compared with those having 677CC genotype. It was shown that the single nucleotide polymorphisms in the MTHFR gene are associated with the risk of gastric carcinoma in the Chinese population.
