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Expression and characterization of mouse lactate dehydrogenase subunit C in Escherichia coli.
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作者 Yong-ZhongXiong De-ZhuZheng FeiXie Xiang-DongTu Feng-HuaLan 《Asian Journal of Andrology》 SCIE CAS CSCD 2004年第1期52-52,共1页
Objective: To construct a prokaryotic recombinant vector for mouse lactate dehydrogenase-C and to detect its expression in BL21. Methods: The coding sequence of mouse lactate dehydrogenase subunit C was amplified from... Objective: To construct a prokaryotic recombinant vector for mouse lactate dehydrogenase-C and to detect its expression in BL21. Methods: The coding sequence of mouse lactate dehydrogenase subunit C was amplified from mouse testis RNA with specific primers and cloned into pGEX-2T after restriction digestion with BamH I and EcoR I. GST fusion protein was expressed after induction with IPTG. Results: Sequencing and restriction digestion of the recombinant plasmid revealed the coding sequence for mouse lactate dehydrogenase subunit C. A protein band of about 60 000 could be induced by IPTG in the recombinant plasmid. Conclusion: The coding sequence of mouse lactate dehydrogenase subunit C was introduced into the pGEX-2T plasmid and a GST-fused protein could be induced at a high level. 展开更多
关键词 lactate dehydrogenase-C SPERMATOZOA CLONING gene expression MOUSE
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