Comparing growth and fine root distribution in monocultures and mixed plantations of hybrid poplar and spruce

Disease prevention, biodiversity, productivity improvement and ecological considerations are all factors that contribute to increasing interest in mixed plantations. The objective of this study was to evaluate early growth and productivity of two hybrid poplar clones, P. balsamifera x trichocarpa （PBT） and P. maximowiczii x balsamifera （PMB）, one improved family of Norway spruce （Picea glauca （PA）） and one improved family of white spruce （Picea abies （PG）） growing under different spacings in monocultures and mixed plots. The plantations were established in 2003 in Abitibi-Témiscamingue, Quebec, Canada, in a split plot design with spacing as the whole plot factor （1 × 1 m, 3 × 3 m and 5 × 5 m） and mixture treatments as subplot factor （pure： PBT, PMB, PA and PG, and 1：1 mixture PBT：PA, PBT：PG, PMB：PA and PMB：PG）. Results showed a beneficial effect of the hybrid poplar-spruce mixture on diameter growth for hybrid poplar clones, but not for the 5 × 5 m spacing because of the relatively young age of the plantations. Diameter growth of the spruces decreased in mixed plantings in the 1 × 1 m, while their height growth increased, resulting in similar aboveground biomass per tree across treatments. Because of the large size differences between spruces and poplars, aboveground biomass in the mixed plantings was generally less than that in pure poplar plots. Leaf nitrogen concentration for the two spruce families and hybrid poplar clone PMB was greater in mixed plots than in monocultures, while leaf nitrogen concentration of clone PBT was similar among mixture treatments. Because of its faster growth rate and greater soil resources demands, clone PMB was the only one showing an increase in leaf N with increased spacing between trees. Fine roots density was greater for both hybrid poplars than spruces. The vertical distribution of fine roots was insensitive to mixture treatment.

How can the forest sector mitigate climate change in a changing climate? Case studies of boreal and northern temperate forests in eastern Canada

Background:Forest based climate mitigation emerged as a key component of the Paris Agreement,and thus re-quires robust science to reduce uncertainties related to such strategies.The aim of this study was to assess and compare the cumulative effects on carbon dynamics of forest management and climate change on boreal and northern temperate forest sector in eastern Canada for the 2020–2100 period.Methods:We used the spatially explicit forest landscape model LANDIS-II and its extension Forest Carbon Suc-cession,in conjunction with the Carbon Budget Model for Harvested Wood Products framework.We simulated the dynamics of forest composition and carbon flows from forest ecosystems to wood products and their substitution effect on markets under increasing climate forcing,according to a tonne-year approach.Simulations were con-ducted for a series of forest management scenarios based on realistic practices principally by clearcut in the boreal territory and continuous-cover forestry in the northern temperate one.These scenarios included:i)a business-as-usual scenario(BaU),representing the current management strategy,ii)increased harvesting by 6.3%to 13.9%,iii)increased conservation(i.e.reduced harvesting by 11.1%to 49.8%),iiii)and a scenario representing the natural evolution of the forest landscape(i.e.without any management activity).Results:Our study revealed that increasing harvesting levels had contrasting effects on the mitigation potential in northern temperate(enhance net sequestration)and boreal forest sector(enhance net emissions)in comparison to the BaU from 2040 onwards,regardless of the future climate.Carbon storage in wood products and the substi-tution effect were not sufficient to offset carbon emissions from ecosystems.Moreover,climate change had a strong impact on the capacity of both landscapes to act as carbon sinks.Northern temperate landscapes became a net source of carbon over time due to their greater vulnerability to climate change than boreal landscapes.Conclusions:Our study highlights the need to consider the initial landscape characteristics in simulations to maximize the mitigation potential of alternative forest management strategies.The optimal management solution can be very different according to the characteristics of forest ecosystems.This opens the possibility of optimizing management for specific forest stands,with the objective of maximizing the mitigation potential of a given landscape.

Global Transcriptional Profile of Tranosema rostrale Ichnovirus Genes in Infected Lepidopteran Hosts and Wasp Ovaries

The ichnovirus TrIV, transmitted by the endoparasitic wasp Tranosema rostrale to its lepidopteran host during oviposition, replicates asymptomatically in wasp ovaries and causes physiological dysfunctions in parasitized caterpillars. The need to identify ichnoviral genes responsible for disturbances induced in lepidopteran hosts has provided the impetus for the sequencing and annotation of ichnovirus genomes, including that of TrIV. In the latter, 86 putative genes were identified, including 35 that could be assigned to recognized ichnoviral gene families. With the aim of assessing the relative importance of each TrIV gene, as inferred from its level of expression, and evaluating the accuracy of the gene predictions made during genome annotation, the present study builds on an earlier qPCR quantification of transcript abundance of TrIV rep ORFs, in both lepidopteran and wasp hosts, extending it to other gene families as well as to a sample of unassigned ORFs. We show that the majority (91%) of putative ORFs assigned to known gene families are expressed in infected larvae, while this proportion is lower (67%) for a sample taken among the remaining ORFs. Selected members of the TrV and rep gene families are shown to be transcribed in infected larvae at much higher levels than genes from any other TrIV gene family, pointing to their likely involvement in host subjugation. In wasp ovaries, the transcriptional profile is dominated by a rep gene and a member of a newly described gene family encoding secreted proteins displaying a novel cysteine motif, which we identified among previously unassigned ORFs.

Holocene variations of wildfire occurrence as a guide for sustainable management of the northeastern Canadian boreal forest

Background: Cumulative impacts of wildfires and forest harvesting can cause shifts from closed-crown forest to open woodland in boreal ecosystems. To lower the probability of occurrence of such catastrophic regime shifts,forest logging must decrease when fire frequency increases, so that the combined disturbance rate does not exceed the Holocene maximum. Knowing how climate warming will affect fire regimes is thus crucial to sustainably manage the forest. This study aimed to provide a guide to determine sustainable forest harvesting levels, by reconstructing the Holocene fire history at the northern limit of commercial forestry in Quebec using charcoal particles preserved in lake sediments.Methods: Sediment cores were sampled from four lakes located close to the northern limit of commercial forestry in Quebec. The cores were sliced into consecutive 0.5 cm thick subsamples from which 1 cm3 was extracted to count and measure charcoal particles larger than 150 microns. Age-depth models were obtained for each core based on accelerator mass spectroscopy(AMS) radiocarbon dates. Holocene fire histories were reconstructed by combining charcoal counts and age-depth models to obtain charcoal accumulation rates and, after statistical treatment,long-term trends in fire occurrence(expressed as number of fires per 1000 years).Results: Fire occurrence varied between the four studied sites, but fires generally occurred more often during warm and dry periods of the Holocene, especially during the Holocene Thermal Maximum(7000–3500 cal. BP), when fire occurrence was twice as high as at present.Conclusions: The current fire regime in the study area is still within the natural range of variability observed over the Holocene. However, climatic conditions comparable to the Holocene Thermal Maximum could be reached within the next few decades, thus substantially reducing the amount of wood available to the forest industry.

Evaluating and quantifying the effect of various spruce budworm intervention strategies on forest carbon dynamics in Atlantic Canada

Spruce budworm (SBW) outbreaks are one of the most devastating natural disturbances in spruce-balsam fir forests of eastern North America. Both early intervention strategy (EIS) and foliage protection strategy (FP) are being tested to limit forest losses, but the quantitative impact on forest carbon (C) dynamics is still unclear. In this study, we designed 19 separate scenarios of no intervention or varying success of EIS, FP, and their combination on SBW caused defoliation and mortality. We then used the TRIPLEX-Insect model to quantify their effects on forest C dynamics in the forests of the four provinces of Atlantic Canada. A scenario applying FP to 10%of the area with the greatest potential C losses of living biomass, protecting foliage in 10%of the forests is more realistic than higher proportion of FP given the high cost and large areas involved, resulted in reducing average cumulative net ecosystem productivity (NEP) from 2020 to 2039 by 56%–127%compared to a no outbreak scenario.Our results showed that FP would have to be applied everywhere to reduce tree mortality and increase NEP more than 8 years of successful EIS applied. However, if EIS can be successfully implemented for 12 years, it will maintain more forest C than FP applied everywhere during a moderate outbreak. We also found that the combination of EIS followed by FP in 10%of the areas disturbed by the SBW could maintain average cumulative NEP at similar levels to no defoliation in every province of Atlantic Canada. Black/red spruce forests younger than 60years old underwent the smallest changes in C dynamics whether using EIS, FP, or both. This highlights the importance of forest species, forest age, and their interactions on the effectiveness of a treatment during SBW outbreak. Overall, 31%–76%of the study area in Atlantic Canada could convert from a C sink to a source by 2039,if no protective measures are used under the worst-case scenarios, thus contributing to future climate warming.

Sexual Dimorphism in the Mandible Shape of <i>Neospondylis upiformis</i>(Coleoptera: Cerambycidae)

Sexes of adult Cerambycidae (Coleoptera) are usually discriminated by the greater length of male antennae. However, in Spondylidinae, adult antennae are short and the difference between sexes is negligible and difficult to appraise. Only two species belong to this subfamily in North America, one of these being Neospondylis upiformis (Mannerheim), a species rarely caught in eastern North America. Unexpectedly, we collected numerous specimens of Neospondylis upiformis on Anticosti Island, Quebec, which appears as a hotspot for this species in eastern Canada. We show that sexual dimorphism in the mandible shape of N. upiformis (Mannerheim) can be used to discriminate sexes. Females have robust mandibles with a sharp cutting inner edge while males have thin mandibles and a well-rounded inner edge. There was no overlap between sexes in all measures done on mandibles, showing that mandible shape was a reliable criterion for sexing N. upiformis. We also tested previously reported criteria using antennae, as well as other characteristics such as body size, and show that they can hardly discriminate between sexes in N. upiformis. We also present illustrations of male and female genitalia, which is rarely available for Cerambycidae.

Soil Agricultural Potential in Four Common Andean Land Use Types in the Highlands of Southern Ecuador as Revealed by a Corn Bioassay

In the Andes, little is known about the relationships among current land uses and their effect on soil fertility. Corn (Zea mays L.) was used to evaluate soil quality for plant growth on soils of four land uses, along an expected gradient of fertility: native forests (Nf) > pastures (Pa) > Eucalyptus globulus Labill. plantations (Eg) > Pinus patula Schlecht. plantations (Pp). Corn was grown in soils taken from four different areas, for the four land uses in each. In a common garden, a randomized block design was used with four treatments: controls (C), ammonium nitrate (N), triple superphosphate (P), and combined N and P fertilizers (N + P). On soils from Nf, Pa and Eg, fertilization response was N + P > P > N > C;corn biomass (g/pot-1) averaged 4.5 in N + P, 3.3 in P, 1.8 in N, 1.7 in C;P content (mg/pot-1) averaged 12 in N + P, 11.9 in P, 2.3 in N, 2 in C. N + P enhanced growth the most. Mortality was high on Pp soils, growth weak, and fertilization response was P > N + P > C ≥ N;corn biomass (g/pot-1) was 0.9 in P, 0.5 in N + P, 0.8 in C, 0.4 in N;P content (mg/pot-1) was 4.4 in P, 2.3 in N + P, 1.8 in C, 1 in N. All soils had P, K, Ca and Mg deficiencies. Al toxicity possibly occurred only in Pp soils. All control soils had low fertility. Responses to N and P were high except for Pp. Pastures and plantations were once natural forests converted to agriculture, then to pastures as soil fertility declined. Plantations were likely established on poorest pastures;only pine grew on poorest soils. This land use endpoint has the lowest agricultural potential;other land uses have limitations in P, N, and potentially K.

The Relative Importance of Natural Disturbances and Local Site Factors on Woody Vegetation Regeneration Diversity across a Large, Contiguous Forest Region

Stand-level diversity after natural disturbance can potentially differ across a large, contiguous forest region despite being dominated by the same canopy species throughout as differences in disturbance types and local site conditions can regulate species distribution. Our main objective was to examine the relative importance of natural disturbances (spruce budworm (Choristoneura fumiferana) outbreak, windthrow, and their interaction) and local site factors (climate, physiography, and stand structure and composition variables) on woody vegetation diversity among three, physiographically distinct locations across a large, contiguous forest region. Seventy-six Abies balsamea-Betula spp. stands affected by natural disturbance were compared and analysed using canonical ordination methods, diversity indices, and ANOVA. Different combinations of factors were important for vegetation re-establishment at each location. Differences in alpha (α), beta (β), gamma (γ), Shannon’s H’, and evenness (J) diversity indices were observed among locations across the study region. Our findings indicate that while certain processes are important for maintaining canopy dominance by Abies balsamea and Betula spp. throughout the region, different combinations of factors were important for creating variation in woody species diversity among locations that resulted in greater woody species diversity at the regional scale.

Cloning, expression and localization of a trypsin-like serine protease in the spruce budworm, Choristoneura fumiferana

A trypsin-like molting-related serine protease cDNA （CfMRSP） was cloned from the spruce budworm, Choristoneurafumiferana. The full-length CfMRSP complementary DNA （cDNA） encoded a 43 kDa protein that contained a trypsin-like serine protease catalytic domain, but no clip domain. The C-terminal extension contained five cystein residues, which may allow the protein to form a homodimer through interchain disulfide bonds and regulate the activity of CfMRSE Phylogenetic tree analysis showed that CfMRSP clusters with lepidopteran homologues such as serine protease 1 of Lonomia obliqua, hemolymph proteinase 20 （HP20）, pattern recognition serine proteinase precursor （ProHP14） and a trypsin-like protein ofManduca sexta. Northern blot analysis of developmental expression of CfMRSP indicated that its transcripts were found primarily in the epidermis and were produced during all of the tested stadia, from 4th instar larvae to pupae, but increased levels of CfMRSP transcripts were always found after each molt. A high level of the protein was found in the epidermis by immunohistochemistry analysis. Altogether these data suggest that CfMRSP plays a role in the epidermis during molting and metamorphosis.

Two sides of a coin： host-plant synchrony fitness trade-offs in the population dynamics of the western spruce budworm

Conifer-feeding budworms emerge from overwintering sites as small larvae in early spring, several days before budburst, and mine old needles. These early-emerging larvae suffer considerable mortality during this foraging period as they disperse in search of available, current-year buds. Once buds flush, surviving budworms construct feeding shelters and must complete maturation before fresh host foliage senesces and lignifies later in the summer. Late-developing larvae suffer greater mortality and survivors have lower fecundity when feeding on older foliage. Thus, there is a seasonal trade-off in fitness associated with host synchrony： early-emerging budworms have a greater risk of mortality during spring dispersal but gain better access to the most nutritious foliage, while, on the other hand, late-emerging larvae incur a lower risk during the initial foraging period but must contend with rapidly diminishing resource quality at the end of the feeding period. We investigate the balance that results from these early-season and late-season synchrony fitness trade-offs using the concept of the phenological window. Parameters associated with the variation in the phenological window are used to estimate generational fitness as a function of host-plant synchrony. Because defoliation modifies these relationships, it is also included in the analysis. We show that fitness trade-offs characterizing the phenological window result in a robust synchrony relationship between budworm and host plant over a wide geographic range in southern British Columbia, Canada.