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Detection of Citrus yellow vein clearing virus by Quantitative Real-time RT-PCR 被引量:4
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作者 CHEN Hongming ZHOU Yan +3 位作者 WANG Xuefeng ZHOU Changyong YANG Xiuyan LI Zhongan 《Horticultural Plant Journal》 SCIE 2016年第4期188-192,共5页
To develop a rapid and reliable detection method for Citrus yellow vein clearing virus(CYVCV), a quantitative real-time reverse transcriptionpolymerase chain reaction(q RT-PCR) system based on SYBR Green I was establi... To develop a rapid and reliable detection method for Citrus yellow vein clearing virus(CYVCV), a quantitative real-time reverse transcriptionpolymerase chain reaction(q RT-PCR) system based on SYBR Green I was established by using a pair of specific primers designed from its conserved coat protein gene. The sensitivity, specificity, and applicability of the system were evaluated accordingly. The results showed that amplicons were produced from CYVCV isolates, whereas no amplicons from non-CYVCV citrus virus samples, including Citrus tristeza virus(CTV) and Citrus tatter leaf virus(CTLV), were obtained. The sensitivity of the q RT-PCR was 100-fold higher than that of conventional RT-PCR. An excellent linear correlation(R2= 0.999) was obtained from two standard curves of c RNA, and the amplification efficiency was 102%. The data from field citrus samples detection showed that the q RT-PCR system could be used in determining the concentration of CYVCV in different citrus species. 展开更多
关键词 CITRUS Citrus yellow vein clearing virus q RT-PCR DETECTION application
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