Ginseng polysaccharides were extracted by water decoction from Panax ginseng C.A.Meyer(Cultivated Ginseng),named CGPS.Four polysaccharide fractions,CGPS-20,CGPS-40,CGPS-60 and CGPS-80,were precipitated at final ethano...Ginseng polysaccharides were extracted by water decoction from Panax ginseng C.A.Meyer(Cultivated Ginseng),named CGPS.Four polysaccharide fractions,CGPS-20,CGPS-40,CGPS-60 and CGPS-80,were precipitated at final ethanol concentrations of 20%,40%,60%and 80%,respectively.Physicochemical properties,molecular weight,monosaccharide composition and antioxidant capacity of polysaccharide fractions were all investigated.The results indicated that changing the concentration of ethanol could precipitate polysaccharides into fractions with different molecular weights,functional group composition and physicochemical properties,eventually leading to differences in antioxidant activity,which would help to find a simple,efficient,and reliable method for rapid extraction and purification of antioxidant polysaccharides from Panax ginseng C.A.Meyer.Among the four polysaccharide fractions,CGPS-80 had lower molecular weight,higher contents of uronic acid and total phenolic,and stronger scavenging ability on DPPH∙and ABTS∙+radicals.展开更多
Four new prenylflavonoid glycosides,namely koreanoside H-K(1-4),together with eleven known ones(5-15)were isolated from the leaves of Epimedium koreanum Nakai.Their structures were elucidated by ID NMR,2D NMR,HR-ESI-M...Four new prenylflavonoid glycosides,namely koreanoside H-K(1-4),together with eleven known ones(5-15)were isolated from the leaves of Epimedium koreanum Nakai.Their structures were elucidated by ID NMR,2D NMR,HR-ESI-MS,IR and UV.The identification of the sugar moieties was carried out by means of acid hydrolysis and HPLC analysis of their derivatives.It is worth noting that compound 3 and compound 4 were elucidated to contain fucose and quinovose moieties,which were two extremely rare sugar units from the genus Epimedium.The anti-pulmonary fibrosis activity of the new compounds was evaluated using A549 cell line.Compounds 1,2 and 4 showed significant anti-pulmonary fibrosis activities.展开更多
基金This work was granted by the National Key R&D Program of China(2017YFC1702302)LiaoNing Revitalization Talents Program(XLYC1902119).
文摘Ginseng polysaccharides were extracted by water decoction from Panax ginseng C.A.Meyer(Cultivated Ginseng),named CGPS.Four polysaccharide fractions,CGPS-20,CGPS-40,CGPS-60 and CGPS-80,were precipitated at final ethanol concentrations of 20%,40%,60%and 80%,respectively.Physicochemical properties,molecular weight,monosaccharide composition and antioxidant capacity of polysaccharide fractions were all investigated.The results indicated that changing the concentration of ethanol could precipitate polysaccharides into fractions with different molecular weights,functional group composition and physicochemical properties,eventually leading to differences in antioxidant activity,which would help to find a simple,efficient,and reliable method for rapid extraction and purification of antioxidant polysaccharides from Panax ginseng C.A.Meyer.Among the four polysaccharide fractions,CGPS-80 had lower molecular weight,higher contents of uronic acid and total phenolic,and stronger scavenging ability on DPPH∙and ABTS∙+radicals.
基金supported by the National Natural Science Foundation of China(Nos.81673548 and U20A20407)Liaoning Revitalization Talents Program(No.XLYC1902119).
文摘Four new prenylflavonoid glycosides,namely koreanoside H-K(1-4),together with eleven known ones(5-15)were isolated from the leaves of Epimedium koreanum Nakai.Their structures were elucidated by ID NMR,2D NMR,HR-ESI-MS,IR and UV.The identification of the sugar moieties was carried out by means of acid hydrolysis and HPLC analysis of their derivatives.It is worth noting that compound 3 and compound 4 were elucidated to contain fucose and quinovose moieties,which were two extremely rare sugar units from the genus Epimedium.The anti-pulmonary fibrosis activity of the new compounds was evaluated using A549 cell line.Compounds 1,2 and 4 showed significant anti-pulmonary fibrosis activities.
