After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembranc sector F0 was specifically extracted from the stripped membranes in t...After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembranc sector F0 was specifically extracted from the stripped membranes in the presence of detergent CHAPS and partially purified. Two-dimensional crystals were produced by the reconstitu-tion of the partially purified F0 into asolectin and microdi-alysis. The obtained crystals are able to diffract to 2 nm. The projection map of the negatively stained crystal shows that the crystal has p4212 symmetry, lattice constant, a = b = 14.4 nm. A unit cell contains four F0 molecules.展开更多
基金This work was supported by the Key Projectof Chinese Academy of Sciences (Grant No. KJ951-A1-601) the National Natural Science Foundation of China (Grant No. 39730130) the Fund of the Chinese Academy of Sciences to Zhang Xujia (Grant No. KSCX2-2-0
文摘After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembranc sector F0 was specifically extracted from the stripped membranes in the presence of detergent CHAPS and partially purified. Two-dimensional crystals were produced by the reconstitu-tion of the partially purified F0 into asolectin and microdi-alysis. The obtained crystals are able to diffract to 2 nm. The projection map of the negatively stained crystal shows that the crystal has p4212 symmetry, lattice constant, a = b = 14.4 nm. A unit cell contains four F0 molecules.
