Bioethanol is a renewable,clean energy and a very important basic chemical raw material,improving production efciency and reducing production cost are the goals pursued by ethanol enterprises.Adding acid protease in t...Bioethanol is a renewable,clean energy and a very important basic chemical raw material,improving production efciency and reducing production cost are the goals pursued by ethanol enterprises.Adding acid protease in the process of producing ethanol from grain will have many efects on fermentation,one of the most concerned efects is to increase liquor yield.Acid protease can hydrolyze the protein in cereals to produce free amino acids that can be used directly by yeast,thus reducing the amount of sugar consumed for amino acid metabolism,and allowing more sugar to be converted to ethanol.The application method and efect of acid protease in the industrial production of ethanol from corn were studied in this study,the results showed that the addition of acid protease at the dosage of 10 U/g(raw material)during yeast seeding could replace urea,promote yeast proliferation,reduce the infection rate,shorten the fermentation period by 7.5 h and increase the alcohol yield of raw materials by 0.33 percentage point.When acid protease was used in ethanol production line with an annual output of 180,000 tons,the net proft increased by at least US$4900/day compared with the control process.It is hoped that this study can lay a foundation for the application of acid protease in ethanol industry.展开更多
Lignocellulose is an abundant and renewable biomass that is mainly composed of cellulose,hemicellulose and lignin.The development and utilization of lignocellulosic ethanol is an efective way to solve the energy probl...Lignocellulose is an abundant and renewable biomass that is mainly composed of cellulose,hemicellulose and lignin.The development and utilization of lignocellulosic ethanol is an efective way to solve the energy problem/crises,In addition,lignocellulosic ethanol industry can play a signifcant role in controlling environmental pollution and extending agricultural industrial chain;however,it has not been able to realize large-scale industrialization due to the limitation of both biotechnology and economy.This review frst introduces industrialization status of lignocellulosic ethanol,and then focuses on the progress of practical technology and analyzing the economic feasibility of the second-generation bioethanol plant;fnally,the future development trend of the industry was prospected.To summarize,continuous technological innovation is needed in vital steps such as pretreatment,enzymatic hydrolysis,and fermentation.Meanwhile,for making the cellulosic ethanol industry truly economically competitive,we also need to achieve interdisciplinary,cross-domain integration innovation,such as the construction of raw material collection and storage system,in situ producing special enzyme system,high-value utilization of raw material components,developing a closely integration of biomass refnery with mature equipment and overall industrialization programs,etc.It is hoped that this review can provide a useful reference for the industrialization of second-generation bioethanol.展开更多
文摘Bioethanol is a renewable,clean energy and a very important basic chemical raw material,improving production efciency and reducing production cost are the goals pursued by ethanol enterprises.Adding acid protease in the process of producing ethanol from grain will have many efects on fermentation,one of the most concerned efects is to increase liquor yield.Acid protease can hydrolyze the protein in cereals to produce free amino acids that can be used directly by yeast,thus reducing the amount of sugar consumed for amino acid metabolism,and allowing more sugar to be converted to ethanol.The application method and efect of acid protease in the industrial production of ethanol from corn were studied in this study,the results showed that the addition of acid protease at the dosage of 10 U/g(raw material)during yeast seeding could replace urea,promote yeast proliferation,reduce the infection rate,shorten the fermentation period by 7.5 h and increase the alcohol yield of raw materials by 0.33 percentage point.When acid protease was used in ethanol production line with an annual output of 180,000 tons,the net proft increased by at least US$4900/day compared with the control process.It is hoped that this study can lay a foundation for the application of acid protease in ethanol industry.
基金This work was supported by the Foundation of State Key Laboratory of Biobased Material and Green Papermaking(No.ZZ20190401),Qilu University of Technology(Shandong Academy of Sciences).
文摘Lignocellulose is an abundant and renewable biomass that is mainly composed of cellulose,hemicellulose and lignin.The development and utilization of lignocellulosic ethanol is an efective way to solve the energy problem/crises,In addition,lignocellulosic ethanol industry can play a signifcant role in controlling environmental pollution and extending agricultural industrial chain;however,it has not been able to realize large-scale industrialization due to the limitation of both biotechnology and economy.This review frst introduces industrialization status of lignocellulosic ethanol,and then focuses on the progress of practical technology and analyzing the economic feasibility of the second-generation bioethanol plant;fnally,the future development trend of the industry was prospected.To summarize,continuous technological innovation is needed in vital steps such as pretreatment,enzymatic hydrolysis,and fermentation.Meanwhile,for making the cellulosic ethanol industry truly economically competitive,we also need to achieve interdisciplinary,cross-domain integration innovation,such as the construction of raw material collection and storage system,in situ producing special enzyme system,high-value utilization of raw material components,developing a closely integration of biomass refnery with mature equipment and overall industrialization programs,etc.It is hoped that this review can provide a useful reference for the industrialization of second-generation bioethanol.
