Objective:To investigate the genetic polymorphism of Plasmodium vivax(P.vivax) PvCSP and PvMSP1 genes from field isolates at four endemic regions(North,East,West and South) of Thailand.Methods:The 152 P.vivax injected...Objective:To investigate the genetic polymorphism of Plasmodium vivax(P.vivax) PvCSP and PvMSP1 genes from field isolates at four endemic regions(North,East,West and South) of Thailand.Methods:The 152 P.vivax injected cases from dried blood spots were DMA extracted and confirmed by species-specific primer sets using multiplex PCR method.PvMSPI fragments F2 and F3:PvCSP were gcnotvped using RFLP-PCR method.Resuits:Totally amplified DNA which was multiple genotypes for PvMSP1 F2 and PvMSP1 F3 were 12.50%and 8.55%.respectively while PvCSP was 3.95%.The overall frequency of multiple genotypes was 25%.There were 12 allele tvpes of PvMSP1 F2 using AluI enzyme digestion and 8 size variations were found in P\MSP1 F3.The isolates from western region was highly genetic diverse when compare among all isolates.The predominant variant type of PvCSP gene was \ K2I0 type.Conclusions:The niulliple genotypes are common found in Thailand and it might hide the real genotype.PvCSP does not have extensive genetic diversity in this study.However.PvMSPI marker due to multiple genotypes is difficult In be analyzed.The multiple genotypes findings might stem from population migration and vector species findings.展开更多
BACKGROUND There is little research investigating how human immunodeficiency virus(HIV)affects outcomes in orthopaedic surgery.With advances in treatment,HIV has become a chronic health problem and the chance of ortho...BACKGROUND There is little research investigating how human immunodeficiency virus(HIV)affects outcomes in orthopaedic surgery.With advances in treatment,HIV has become a chronic health problem and the chance of orthopaedic surgeons encountering it in clinical practice is increasing.AIM To ascertain the quantity and quality of peer-reviewed publications in orthopaedic journals about HIV.METHODS A search of the Web of Science database was carried out,identifying any articles relating to HIV published in orthopaedic journals.These were assessed for geographic origin and level of evidence.RESULTS Of 48.7%of orthopaedic journals listed on the Web of Science database had published articles relating to HIV.There were 168 articles about HIV in orthopaedic journals with only 40.5%(n=68)published in the time frame we analysed(January 2007 to September 2017).Very few articles came from lowincome countries and any articles published from that setting were collaborations.All of the articles were low level of evidence.CONCLUSION There is a need for more high level orthopaedic and trauma research investigating the effects of HIV,particularly research from low-income countries,where higher level research will help to guide improvements in their treatment of its musculoskeletal manifestations and complications.展开更多
Objective:To investigate possible protein targets for antimalarial activity of Garcina mangostana Linn.(G.mangostana)(pericarp)in 3D7 Plasmodium falciparum clone using 2-dimensional electrophoresis and liquid chromato...Objective:To investigate possible protein targets for antimalarial activity of Garcina mangostana Linn.(G.mangostana)(pericarp)in 3D7 Plasmodium falciparum clone using 2-dimensional electrophoresis and liquid chromatography mass-spectrometry(LC/MS/MS).Methods:3D7 Plasmodium falciparum was exposed to the crude ethanolic extract of G.mangostana Linn.(pericarp)at the concentrations of 12μg/mL(1C_(50)level:concentration that inhibits parasite growth by 50%)and 30μg/mL(1C_(90)level:concentration that inhibits parasite growth by 90%)for 12 h.Parasite proteins were separated by 2-dimensional electrophoresis and identified by LC/MS/MS.Results:At the IC_(50)concentration,about 82%of the expressed parasite proteins were matched with the control(non-exposed),while at the IC_(90)concentration,only 15%matched proteins were found.The selected protein spots from parasite exposed to the plant extract at the concentration of 12μg/mL were identified as eneymes that play role in glycolysis pathway,i.e.,phosphoglyeerate mutase putative,L-lactate dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase,and fruetose-bisphosphate aldolase/phosphoglyeerate kinase.The proteosome was found in parasite exposed to 30μg/mL of the extract.Conclusions:Results suggest that proteins involved in the glycolysis pathway may be the targets for antimalarial activity of G.mangostana Linn.(pericarp).展开更多
Objective:To explore the efficacy of intermittent preventive treatment in pregnancy(IPTp)with sulfadoxine and pyrimethamine(SP)against sensitive parasites.Methods:A pharmacological model was used to investigate the ef...Objective:To explore the efficacy of intermittent preventive treatment in pregnancy(IPTp)with sulfadoxine and pyrimethamine(SP)against sensitive parasites.Methods:A pharmacological model was used to investigate the effectiveness of the previous recommended at least two-dose regimen,currently recommended three-dose regimen and 4,6,8-weekly regimens with specific focus on the impact of various nonadherence patterns in multiple transmission settings.Results:The effectiveness of the recommended three-dose regimen is high in all the transmission intensities,i.e.>99%,98%and 92%in low,moderate and high transmission intensities respectively.The simulated 4 and 6 weekly IPTp-SP regimens were able to prevent new infections with sensitive parasites in almost all women(>99%)regardless of transmission intensity.However,8 weekly interval dose schedules were found to have 71%and 86%protective efficacies in high and moderate transmission areas,respectively.It highlights that patients are particularly vulnerable to acquiring new infections if IPTp-SP doses are missed.Conclusions:The pharmacological model predicts that full adherence to the currently recommended three-dose regimen should provide almost complete protection from malaria infection in moderate and high transmission regions.However,it also highlights that patients are particularly vulnerable to acquiring new infections if IPTp doses are spaced too widely or if doses are missed.Adherence to the recommended IPTp-SP schedules is recommended.展开更多
Background: Childhood tuberculosis accounts for about 10% of estimated TB cases in the world. Despite advances in diagnostics, childhood TB remains a challenge. We evaluated pooling method and testing with GeneXpert M...Background: Childhood tuberculosis accounts for about 10% of estimated TB cases in the world. Despite advances in diagnostics, childhood TB remains a challenge. We evaluated pooling method and testing with GeneXpert MTB/RIF in southern Ethiopia. Methods: This is a cross-sectional study in presumptive TB children st, 2nd and pooled samples. Results: Of 340 presumptive TB cases enrolled, 96 and 244 children submitted gastric aspirate and sputum samples respectively. Of 1020 samples collected (282 gastric aspirate and 738 sputum samples), 38 (3.7%) were positive by Xpert (10 (3.5%) from gastric aspirate and 28 (3.8%) from sputum sample). Similarly, 8 (1.2%) of sputum samples were positive by ZN but none from gastric aspirate. Of 244 children who submitted sputum samples, 3 (1.2%) were bacteriologically positive compared to 12 (4.9%) by Xpert. Of 96 children who submitted gastric aspirate samples, none were positive by ZN while 5 (5.2%) were positive by Xpert. Of bacteriologically confirmed TB cases 0.9% was by ZN and 4.7% by Xpert, an increase of 3.8%. Pooled testing increased positivity by 0.3% for ZN and 1.5% by Xpert compared to the 1st sample. Conclusions: Xpert MTB/RIF testing increases yield compared to ZN testing for gastric aspirate samples. The same-day approach and pooling samples improves efficient use of cartridge, reduce the number of visits for seeking diagnosis and save resources.展开更多
Isoniazid preventive therapy(IPT) is the administration of isoniazid(INH) to people with latent tuberculosis(TB) infection(LTBI) to prevent progression to active TB disease. Despite being life-saving for human immunod...Isoniazid preventive therapy(IPT) is the administration of isoniazid(INH) to people with latent tuberculosis(TB) infection(LTBI) to prevent progression to active TB disease. Despite being life-saving for human immunodeficiency virus(HIV)-infected persons who do not have active TB, IPT is poorly implemented globally due to misconceptions shared by healthcare providers and policy makers. However, amongst HIV-infected patients especially those living in resource-limited settings with a high burden of TB, available evidence speaks for IPT: Among HIV-infected persons, active TB- the major contraindication to IPT, can be excluded with symptom screening; chest X-ray and tuberculin skin testing are unreliable and often lead to logistic delays resulting in increased numbers of people with LTBI progressing to active TB; the use of IPT has not been found to increase the risk of the development of INH mono-resistance; IPT is cost-effective and cheaper than the cost of treating cases of active TB that would develop without IPT; ART and IPT have an additive effect on the prevention of TB, and both are safe and beneficial even in children. In order to sustain the recorded gains from ART scale-up and to further reduce TB-related morbidity and mortality, more efforts are needed to scale-up IPT implementation globally.展开更多
<strong>Introduction: </strong>Stress and burnout are a reality which nurses encounter and try to cope with especially in the Emergency Department (ED) so that they can provide optimal patient care. Nurses...<strong>Introduction: </strong>Stress and burnout are a reality which nurses encounter and try to cope with especially in the Emergency Department (ED) so that they can provide optimal patient care. Nurses who work in ED are front line providers of immediate medical care needed to stabilize patients. With the known critical shortage of health workforce in Cameroon, nurses are overloaded with work and often experience stress and burnout. <strong>Aim: </strong>This study aimed at determining the causes, effects and management strategies of stress and burnout among nurses working in the ED in hospitals in the Fako Division, Cameroon. <strong>Methods:</strong> This study was a descriptive cross-sectional study. The sample consisted of seventy nurses from five different hospitals. A purposive sampling technique was used and data was analyzed using SPSS version 16.0. Data was collected using a structured questionnaire developed from the International Stress Management Scale. <strong>Results: </strong>The highest reported cause of stress in this study was heavy workload (12.88%) and the least was no experience in handling the challenges of the department. The leading reported effect of stress was the development of musculoskeletal disorders such as joint and back pain (16.48%) and the least effect was contemplating quitting the profession (5.99%). The major management strategy used was humour (8.27%), while the least was crying out stress to feel relieved (2.18%).<strong> Conclusion: </strong>The major cause of stress and burnout among nurses in our study setting was heavy workload, which mostly results in joint and back pain. Moreover, humour was the prime management strategy for stress and burnout among nurses in the emergency department in the study setting. Thus more nurses should be employed and the working conditions of nurses improved to reduce the workload in the study hospitals.展开更多
Information on Culex mosquitoes (vectors of filarial worm and viral encephalitis) from northern Nigeria is scanty, hindering evidence-based control. Here, two Culex populations (Kano and Kaduna) were characterized. Cu...Information on Culex mosquitoes (vectors of filarial worm and viral encephalitis) from northern Nigeria is scanty, hindering evidence-based control. Here, two Culex populations (Kano and Kaduna) were characterized. Culex quinquefasciatus and Culex pipiens were found breeding in sympatry, with some hybrid individuals identified. Larval bioassays revealed high temephos resistance (LC<sub>50</sub>s = 1.34 mg/mL and 3.01 mg/mL for Kano and Kaduna, respectively). Larvae were more sensitive to α-cypermethrin (LC<sub>50</sub>s = 0.026 mg/mL and 0.067 mg/mL for Kano and Kaduna). WHO adult tube bioassays revealed high pyrethroid and DDT resistance, with mortalities of 44.01% ± 6.79%, 35.83% ± 12.58%, 29.69% ± 9.97% and 52.47% ± 4.34% for permethrin, deltamethrin, α-cypermethrin and DDT, respectively. Highest resistance was observed with bendiocarb (mortality = 13.58% ± 3.98%). High resistance was obtained with fenitrothion and malathion (mortalities = 21% ± 4.76% and 56.47% ± 8.67%, respectively), while a full susceptibility was observed with pirimiphos-methyl. Pre-exposure to piperonylbutoxide (PBO) significantly recovered α-cypermethrin susceptibility (mortality = 82% ± 5.16%, χ<sup>2</sup> = 50.99, p < 0.0001), compared with the conventional bioassay (mortality = 32 ± 7.30). Mortalities of <20% were obtained in cone bioassays with Yorkool, DuraNet and PermaNet3.0 (side panels) nets, suggesting a loss of efficacy of conventional long-lasting insecticidal nets. However, mortalities of 99% and 86% were obtained in Kano and Kaduna populations using the roof of PermaNet3.0 (containing PBO and deltamethrin). Despite the high frequency of the 1014F VGSC knockdown resistance mutation allele (0.90), no correlation was observed between the 1014F kdr genotype and resistance phenotype. Sequencing of fragments of the acetylcholinesterase-1 gene detected no G119S mutation, in malathion-alive and malathion-dead females. These suggest a preeminent role of metabolic resistance in these Culex populations.展开更多
<strong>Background: </strong>Low and Middle-Income Countries (LMIC) account for 94% of maternal deaths annually. Interventions to reduce these deaths include;access to Emergency Obstetric Care (EmOC) and S...<strong>Background: </strong>Low and Middle-Income Countries (LMIC) account for 94% of maternal deaths annually. Interventions to reduce these deaths include;access to Emergency Obstetric Care (EmOC) and Skilled Birth Attendant (SBA) at childbirth. However, evidence indicates increasing access to EmOC, and SBA only does not translate into positive maternal and newborn outcome due to disrespectful care faced by women during labour. World Health Organization (WHO) guidelines emphasize on positive birth experience through Respectful Maternity Care (RMC). Therefore, this review aims to explore enablers and barriers to respectful maternity care in low and middle-income countries. <strong>Methods:</strong> We conducted an exhaustive literature search for studies that reported on enablers and barriers to respectful maternity care. Qualitative studies done in low and middle-income countries, published in English Language from the year 2000 to June 2020 were included in this study. Articles were screened by two researchers for eligibility and critical appraisal skills programme checklist was used to appraise the quality. The themes and quotes from the studies were extracted and synthesized using thematic synthesis. <strong>Results: </strong>The search strategy generated 14,190 articles and 54 studies met the inclusion criteria. Two main themes: interpersonal relationship and support, and privacy and confidential care were reported as both enablers and barriers to respectful maternity care. Strategies to promote RMC were: health education to pregnant women on care expected during labour, good communication between maternity staff and women, capacity building of staff on RMC and staff motivation. <strong>Conclusion:</strong> Respectful maternity care plays a big role in promoting health-seeking behaviours among pregnant women. However, women experience barriers ranging from provider behaviour, work environment and health system challenges. Ensuring a dignified and respectful working environment could contribute to an increase in health seeking-behaviours and consequently reduction of maternal mortality.展开更多
<b>Background:</b> Good knowledge of diseases’ epidemiology, diagnostic methods and treatment schemes are critical for early detection and effective management of dengue and chikungunya outbreaks. Here, w...<b>Background:</b> Good knowledge of diseases’ epidemiology, diagnostic methods and treatment schemes are critical for early detection and effective management of dengue and chikungunya outbreaks. Here, we assessed knowledge and practices regarding dengue and chikungunya among health care workers of some health facilities of Yaoundé and its surroundings. <b>Methods:</b> A descriptive cross-sectional study was conducted from January to August 2020 in 35 health facilities of Yaoundé and its surroundings. Structured pre-tested questionnaire including 11 questions was used to assess healthcare workers’ (i) knowledge on dengue and chikungunya (pathogen, mode of transmission, signs and symptoms) and, (ii) management practices (diagnosis, prevention and treatment measures). Face to face interviews were conducted with healthcare workers including medical doctors, nurses, medical assistants and laboratory assistants. <b>Findings:</b> A total of 232 healthcare workers were interviewed. Despite that 40.95% (n = 95) of respondents had heard about dengue and chikungunya, only 4.74% (n = 11) of them were aware of the fact that both diseases are caused by virus. Also, 9 out of 232 (3.88%) knew that these diseases were transmitted by the bite of an Aedes mosquito. About 15.52% (n = 36) were able to give at least 1 clinical symptom related to dengue and chikungunya. The main diagnostic method they knew was Polymerase Chain Reaction (3.02%;n = 7). For prevention, many participants indicated vector control by using insecticide treated bednets or destroying potential breeding sites (14.23%;n = 33). Only few participants 3.88% (n = 9) knew how to manage dengue or chikungunya cases. To improve the management of both diseases, a significant proportion of healthcare workers (37.93%;n = 88) reported being in need of additional training. <b>Conclusion:</b> The poor knowledge regarding dengue and chikungunya among healthcare workers in Yaoundé and its neighbourhood raises the need to intensify sensitization campaigns and training activities towards this personnel.展开更多
<b><span style="font-family:Verdana;">Background:</span></b></span><span><span><span style="font-family:""><span style="font-family:Verd...<b><span style="font-family:Verdana;">Background:</span></b></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> Understanding the biology of </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> malaria vector species is essential to planning effective and sustainable malaria control strategies in endemic countries. This study reported the implication of </span><i><span style="font-family:Verdana;">Anopheles leesoni </span></i><span style="font-family:Verdana;">in malaria transmission in Cameroon, Central Africa. </span><b><span style="font-family:Verdana;">Methods:</span></b><i> </i><span style="font-family:Verdana;">Mosquitoes were collected in three localities from May 2015 to March 2018 using electric aspirators and Centers for Disease Control light traps (CDC-LT). </span><i><span style="font-family:Verdana;">Anopheles funestus</span></i> <i><span style="font-family:Verdana;">sensu lato</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">.) mosquitoes were identified as species using polymerase chain reaction assay (PCR). Furthermore, </span><i><span style="font-family:Verdana;">Plasmodium falciparum</span></i><span style="font-family:Verdana;"> infection status was determined using the enzyme-linked</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">immunosorbent assay (ELISA) method. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A total of 12,744 </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> mosquitoes were collected by electric aspirator (N = 4844) and CDC-LT (N = 7900). </span><i><span style="font-family:Verdana;">Anopheles funestus</span></i><span> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. (86.95%)</span><i> </i></span><span style="font-family:Verdana;">was the major species and the main malaria vector in rural savannah and rural forest sites followed by </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">gambiae</span></i><span> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. (13.05%)</span></span><span style="font-family:Verdana;"> whereas</span><span><span style="font-family:Verdana;"> in urban areas, </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> gambiae</span></i> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. was</span></span><span style="font-family:Verdana;"> by far the most abundant representing 91.45% of </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> mosquitoes collected. Two members of the </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus</span></i><span style="font-family:Verdana;"> group were identified among 1389 analysed by PCR: 1307 </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus sensu stricto</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.)</span><i> </i><span style="font-family:Verdana;">(94.10%) and 82 </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i><span style="font-family:Verdana;"> (5.9%). </span><i><span style="font-family:Verdana;">Plasmodium falciparum </span></i><span style="font-family:Verdana;">infection rate was 21.04% in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus </span></i></span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">. For the first time, </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i> </span><span style="font-family:Verdana;">was found positive for </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i><span style="font-family:Verdana;"> (infection rate: 10.98%)</span></span><span style="font-family:Verdana;"> in Cameroon. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">A very high </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i></span><span style="font-family:Verdana;"> infection rate was observed in this study in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i></span><span style="font-family:Verdana;">., highlighting its high implication in malaria transmission in Cameroon. Furthermore, the detection of </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i></span><span style="font-family:Verdana;"> infection in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i></span><span style="font-family:Verdana;"> calls for more attention towards this neglected vector species.展开更多
<strong>Background: </strong><span><span><span><span>With the rapid expansion of insecticide resistance limiting the effectiveness of insecticide-based vector control interventions,...<strong>Background: </strong><span><span><span><span>With the rapid expansion of insecticide resistance limiting the effectiveness of insecticide-based vector control interventions, integrated control strategies associating larviciding could be appropriate to improve current control efforts. The present experimental study assesses laboratory and field efficacy of the larvicide </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> on <i>Anopheline</i> and <i>Culicine</i> larval stages in Yaoundé. <strong>Methods:</strong> The effect of the larvicide </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G,</span></span></span></span><span><span><span><span> a combination of <i>Bacillus</i><span> <i>thuringiensis</i> var. <i>israelensis</i> </span>(<i>Bti</i>) </span></span></span></span><span><span><span><span>and <i>Bacillus</i> <i>sphaericus</i> (<i>Bs</i>),</span></span></span></span><span><span><span><span> on larval development was assessed during both laboratory and open field trial experiments. Laboratory experiments permitted the evaluation of five different concentrations with four replicates/experiments. Laboratory experiments were conducted with <i>Anopheles</i> <i>coluzzii</i> “Ngousso” and <i>Culex</i> <i>quinquefasciatus</i> laboratory strains. Open field trials were conducted using </span></span></span></span><span><span><span><span>sixteen plastic containers with a diameter of 0.31 m buried in an array of four rows with 4 containers each. Distance between rows and between containers in a row was 1 meter. This experiment permitted to </span></span></span></span><span><span><span><span>test the effect of the microbial larvicide </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> under operational application conditions on field mosquito populations. <strong>Results:</strong> <span>The time to induce 100% mortality after exposure to serial concentrations of the larvicide varied according to the dose from 4 - 12 hours for <i>An.</i> <i>coluzzii</i> and 6 - 9 hours for <i>Cx.</i> <i>quinquefasciatus</i> in laboratory experiments. Measurements of the</span> residual activity indicated that all </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> concentrations were still active after 35 days and killed 86</span></span></span></span><span><span><span><span>% </span></span></span></span><span><span><span><span>-</span></span></span></span><span><span><span><span> </span></span></span></span><span><span><span><span>100% of larvae. Lethal dose of </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> killing 50% of larvae was estimated at 5.24 × 10<sup>-8</sup> mg/m<sup>2</sup> for <i>An.</i> <i>coluzzii</i> and 1.25 × 10<sup>-8</sup> mg/m<sup>2</sup> for <i>Cx.</i> <i>quinquefasciatus</i>. The lethal concentration inducing 95% mortality was estimated at 3.13 × 10<sup>-7</sup> mg/m<sup>2</sup> for <i>An.</i> <i>coluzzii</i> and 2.5 × 10<sup>-8</sup> <span>mg/m<sup>2</sup> for <i>Cx.</i> <i>quinquefasciatus</i>. Open field trials tests indicated that </span>sub-lethal concentrations of </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> successfully killed 100% <i>An.</i> <i>gambiae</i> s.l. larvae within 24 hours, while with <i>Culex</i> spp. larvae, 100% mortality was recorded after 48 hours post-treatment. Natural recolonization of water containers by larvae was recorded between 3 and 6 days respectively after the treatment with sublethal doses. Late instar larvae were recorded 5 and 6 days after treatment. When the jars were treated with reference dosage or supra doses of </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G,</span></span></span></span><span><span><span><span> recolonization of water containers was observed six days after treatments. No pupae of both species were found 6 and 7 days post-treatment. <strong>Conclusions:</strong> The study indicated high efficacy of the microbial larvicide </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> against <i>Anopheline</i> and <i>Culex</i> larvae. Microbial larvicides such as </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> could be appropriate for controlling mosquito population particularly in areas experiencing high insecticide resistance or outdoor biting mosquitoes.</span></span></span></span>展开更多
研究院长负责制的实施北景、内容及对人员表现的影响。选择社会经济水平不同的B县(较富裕)和C县(较贫困)两个县进行调查,以召开专题小组讨论(focus group discussion)和知情者访谈(key informant interview)的定性调查方法为主,结合医...研究院长负责制的实施北景、内容及对人员表现的影响。选择社会经济水平不同的B县(较富裕)和C县(较贫困)两个县进行调查,以召开专题小组讨论(focus group discussion)和知情者访谈(key informant interview)的定性调查方法为主,结合医院收支、诊疗等方面的定量数据进行分析。结果提示院长负责制受到院长、卫技人员的普遍拥护,调动院长与职工的工作积极性。实行院长负责制期间,两个县医院的医疗活动、经济创收、满意度与人员培训方面均有所改善。分析“放权”政策的局限性以及改善人员表现应采取的措施。讨论实行并完善院长负责制要正确处理扩大自主权和强化束约力的辩证关系和正确认识与理解新形势下的院长负责制等问题。展开更多
基金supported by "Strategic Scholarships Fellowships Fromtier Research Network"from the Commission Higher Education(Thailand) (Grant No.CHE-PhD-SW-INV-20060169)also parially supported by the China Medical Board,Faculty of Public Health,Mahidol University,Bangkok,Thailand
文摘Objective:To investigate the genetic polymorphism of Plasmodium vivax(P.vivax) PvCSP and PvMSP1 genes from field isolates at four endemic regions(North,East,West and South) of Thailand.Methods:The 152 P.vivax injected cases from dried blood spots were DMA extracted and confirmed by species-specific primer sets using multiplex PCR method.PvMSPI fragments F2 and F3:PvCSP were gcnotvped using RFLP-PCR method.Resuits:Totally amplified DNA which was multiple genotypes for PvMSP1 F2 and PvMSP1 F3 were 12.50%and 8.55%.respectively while PvCSP was 3.95%.The overall frequency of multiple genotypes was 25%.There were 12 allele tvpes of PvMSP1 F2 using AluI enzyme digestion and 8 size variations were found in P\MSP1 F3.The isolates from western region was highly genetic diverse when compare among all isolates.The predominant variant type of PvCSP gene was \ K2I0 type.Conclusions:The niulliple genotypes are common found in Thailand and it might hide the real genotype.PvCSP does not have extensive genetic diversity in this study.However.PvMSPI marker due to multiple genotypes is difficult In be analyzed.The multiple genotypes findings might stem from population migration and vector species findings.
文摘BACKGROUND There is little research investigating how human immunodeficiency virus(HIV)affects outcomes in orthopaedic surgery.With advances in treatment,HIV has become a chronic health problem and the chance of orthopaedic surgeons encountering it in clinical practice is increasing.AIM To ascertain the quantity and quality of peer-reviewed publications in orthopaedic journals about HIV.METHODS A search of the Web of Science database was carried out,identifying any articles relating to HIV published in orthopaedic journals.These were assessed for geographic origin and level of evidence.RESULTS Of 48.7%of orthopaedic journals listed on the Web of Science database had published articles relating to HIV.There were 168 articles about HIV in orthopaedic journals with only 40.5%(n=68)published in the time frame we analysed(January 2007 to September 2017).Very few articles came from lowincome countries and any articles published from that setting were collaborations.All of the articles were low level of evidence.CONCLUSION There is a need for more high level orthopaedic and trauma research investigating the effects of HIV,particularly research from low-income countries,where higher level research will help to guide improvements in their treatment of its musculoskeletal manifestations and complications.
基金supported by The National Research Council of Thailand.(Grant No.034/2556)Thailand Research Fund,(Grant No.MRG5380192)
文摘Objective:To investigate possible protein targets for antimalarial activity of Garcina mangostana Linn.(G.mangostana)(pericarp)in 3D7 Plasmodium falciparum clone using 2-dimensional electrophoresis and liquid chromatography mass-spectrometry(LC/MS/MS).Methods:3D7 Plasmodium falciparum was exposed to the crude ethanolic extract of G.mangostana Linn.(pericarp)at the concentrations of 12μg/mL(1C_(50)level:concentration that inhibits parasite growth by 50%)and 30μg/mL(1C_(90)level:concentration that inhibits parasite growth by 90%)for 12 h.Parasite proteins were separated by 2-dimensional electrophoresis and identified by LC/MS/MS.Results:At the IC_(50)concentration,about 82%of the expressed parasite proteins were matched with the control(non-exposed),while at the IC_(90)concentration,only 15%matched proteins were found.The selected protein spots from parasite exposed to the plant extract at the concentration of 12μg/mL were identified as eneymes that play role in glycolysis pathway,i.e.,phosphoglyeerate mutase putative,L-lactate dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase,and fruetose-bisphosphate aldolase/phosphoglyeerate kinase.The proteosome was found in parasite exposed to 30μg/mL of the extract.Conclusions:Results suggest that proteins involved in the glycolysis pathway may be the targets for antimalarial activity of G.mangostana Linn.(pericarp).
基金funded by the Bill and Melinda Gates Foundation(grant No.37999.01)the Medical Research Council(grant No.G110052)supported by the Liverpool School of Tropical Medicine
文摘Objective:To explore the efficacy of intermittent preventive treatment in pregnancy(IPTp)with sulfadoxine and pyrimethamine(SP)against sensitive parasites.Methods:A pharmacological model was used to investigate the effectiveness of the previous recommended at least two-dose regimen,currently recommended three-dose regimen and 4,6,8-weekly regimens with specific focus on the impact of various nonadherence patterns in multiple transmission settings.Results:The effectiveness of the recommended three-dose regimen is high in all the transmission intensities,i.e.>99%,98%and 92%in low,moderate and high transmission intensities respectively.The simulated 4 and 6 weekly IPTp-SP regimens were able to prevent new infections with sensitive parasites in almost all women(>99%)regardless of transmission intensity.However,8 weekly interval dose schedules were found to have 71%and 86%protective efficacies in high and moderate transmission areas,respectively.It highlights that patients are particularly vulnerable to acquiring new infections if IPTp-SP doses are missed.Conclusions:The pharmacological model predicts that full adherence to the currently recommended three-dose regimen should provide almost complete protection from malaria infection in moderate and high transmission regions.However,it also highlights that patients are particularly vulnerable to acquiring new infections if IPTp doses are spaced too widely or if doses are missed.Adherence to the recommended IPTp-SP schedules is recommended.
文摘Background: Childhood tuberculosis accounts for about 10% of estimated TB cases in the world. Despite advances in diagnostics, childhood TB remains a challenge. We evaluated pooling method and testing with GeneXpert MTB/RIF in southern Ethiopia. Methods: This is a cross-sectional study in presumptive TB children st, 2nd and pooled samples. Results: Of 340 presumptive TB cases enrolled, 96 and 244 children submitted gastric aspirate and sputum samples respectively. Of 1020 samples collected (282 gastric aspirate and 738 sputum samples), 38 (3.7%) were positive by Xpert (10 (3.5%) from gastric aspirate and 28 (3.8%) from sputum sample). Similarly, 8 (1.2%) of sputum samples were positive by ZN but none from gastric aspirate. Of 244 children who submitted sputum samples, 3 (1.2%) were bacteriologically positive compared to 12 (4.9%) by Xpert. Of 96 children who submitted gastric aspirate samples, none were positive by ZN while 5 (5.2%) were positive by Xpert. Of bacteriologically confirmed TB cases 0.9% was by ZN and 4.7% by Xpert, an increase of 3.8%. Pooled testing increased positivity by 0.3% for ZN and 1.5% by Xpert compared to the 1st sample. Conclusions: Xpert MTB/RIF testing increases yield compared to ZN testing for gastric aspirate samples. The same-day approach and pooling samples improves efficient use of cartridge, reduce the number of visits for seeking diagnosis and save resources.
文摘Isoniazid preventive therapy(IPT) is the administration of isoniazid(INH) to people with latent tuberculosis(TB) infection(LTBI) to prevent progression to active TB disease. Despite being life-saving for human immunodeficiency virus(HIV)-infected persons who do not have active TB, IPT is poorly implemented globally due to misconceptions shared by healthcare providers and policy makers. However, amongst HIV-infected patients especially those living in resource-limited settings with a high burden of TB, available evidence speaks for IPT: Among HIV-infected persons, active TB- the major contraindication to IPT, can be excluded with symptom screening; chest X-ray and tuberculin skin testing are unreliable and often lead to logistic delays resulting in increased numbers of people with LTBI progressing to active TB; the use of IPT has not been found to increase the risk of the development of INH mono-resistance; IPT is cost-effective and cheaper than the cost of treating cases of active TB that would develop without IPT; ART and IPT have an additive effect on the prevention of TB, and both are safe and beneficial even in children. In order to sustain the recorded gains from ART scale-up and to further reduce TB-related morbidity and mortality, more efforts are needed to scale-up IPT implementation globally.
文摘<strong>Introduction: </strong>Stress and burnout are a reality which nurses encounter and try to cope with especially in the Emergency Department (ED) so that they can provide optimal patient care. Nurses who work in ED are front line providers of immediate medical care needed to stabilize patients. With the known critical shortage of health workforce in Cameroon, nurses are overloaded with work and often experience stress and burnout. <strong>Aim: </strong>This study aimed at determining the causes, effects and management strategies of stress and burnout among nurses working in the ED in hospitals in the Fako Division, Cameroon. <strong>Methods:</strong> This study was a descriptive cross-sectional study. The sample consisted of seventy nurses from five different hospitals. A purposive sampling technique was used and data was analyzed using SPSS version 16.0. Data was collected using a structured questionnaire developed from the International Stress Management Scale. <strong>Results: </strong>The highest reported cause of stress in this study was heavy workload (12.88%) and the least was no experience in handling the challenges of the department. The leading reported effect of stress was the development of musculoskeletal disorders such as joint and back pain (16.48%) and the least effect was contemplating quitting the profession (5.99%). The major management strategy used was humour (8.27%), while the least was crying out stress to feel relieved (2.18%).<strong> Conclusion: </strong>The major cause of stress and burnout among nurses in our study setting was heavy workload, which mostly results in joint and back pain. Moreover, humour was the prime management strategy for stress and burnout among nurses in the emergency department in the study setting. Thus more nurses should be employed and the working conditions of nurses improved to reduce the workload in the study hospitals.
文摘Information on Culex mosquitoes (vectors of filarial worm and viral encephalitis) from northern Nigeria is scanty, hindering evidence-based control. Here, two Culex populations (Kano and Kaduna) were characterized. Culex quinquefasciatus and Culex pipiens were found breeding in sympatry, with some hybrid individuals identified. Larval bioassays revealed high temephos resistance (LC<sub>50</sub>s = 1.34 mg/mL and 3.01 mg/mL for Kano and Kaduna, respectively). Larvae were more sensitive to α-cypermethrin (LC<sub>50</sub>s = 0.026 mg/mL and 0.067 mg/mL for Kano and Kaduna). WHO adult tube bioassays revealed high pyrethroid and DDT resistance, with mortalities of 44.01% ± 6.79%, 35.83% ± 12.58%, 29.69% ± 9.97% and 52.47% ± 4.34% for permethrin, deltamethrin, α-cypermethrin and DDT, respectively. Highest resistance was observed with bendiocarb (mortality = 13.58% ± 3.98%). High resistance was obtained with fenitrothion and malathion (mortalities = 21% ± 4.76% and 56.47% ± 8.67%, respectively), while a full susceptibility was observed with pirimiphos-methyl. Pre-exposure to piperonylbutoxide (PBO) significantly recovered α-cypermethrin susceptibility (mortality = 82% ± 5.16%, χ<sup>2</sup> = 50.99, p < 0.0001), compared with the conventional bioassay (mortality = 32 ± 7.30). Mortalities of <20% were obtained in cone bioassays with Yorkool, DuraNet and PermaNet3.0 (side panels) nets, suggesting a loss of efficacy of conventional long-lasting insecticidal nets. However, mortalities of 99% and 86% were obtained in Kano and Kaduna populations using the roof of PermaNet3.0 (containing PBO and deltamethrin). Despite the high frequency of the 1014F VGSC knockdown resistance mutation allele (0.90), no correlation was observed between the 1014F kdr genotype and resistance phenotype. Sequencing of fragments of the acetylcholinesterase-1 gene detected no G119S mutation, in malathion-alive and malathion-dead females. These suggest a preeminent role of metabolic resistance in these Culex populations.
文摘<strong>Background: </strong>Low and Middle-Income Countries (LMIC) account for 94% of maternal deaths annually. Interventions to reduce these deaths include;access to Emergency Obstetric Care (EmOC) and Skilled Birth Attendant (SBA) at childbirth. However, evidence indicates increasing access to EmOC, and SBA only does not translate into positive maternal and newborn outcome due to disrespectful care faced by women during labour. World Health Organization (WHO) guidelines emphasize on positive birth experience through Respectful Maternity Care (RMC). Therefore, this review aims to explore enablers and barriers to respectful maternity care in low and middle-income countries. <strong>Methods:</strong> We conducted an exhaustive literature search for studies that reported on enablers and barriers to respectful maternity care. Qualitative studies done in low and middle-income countries, published in English Language from the year 2000 to June 2020 were included in this study. Articles were screened by two researchers for eligibility and critical appraisal skills programme checklist was used to appraise the quality. The themes and quotes from the studies were extracted and synthesized using thematic synthesis. <strong>Results: </strong>The search strategy generated 14,190 articles and 54 studies met the inclusion criteria. Two main themes: interpersonal relationship and support, and privacy and confidential care were reported as both enablers and barriers to respectful maternity care. Strategies to promote RMC were: health education to pregnant women on care expected during labour, good communication between maternity staff and women, capacity building of staff on RMC and staff motivation. <strong>Conclusion:</strong> Respectful maternity care plays a big role in promoting health-seeking behaviours among pregnant women. However, women experience barriers ranging from provider behaviour, work environment and health system challenges. Ensuring a dignified and respectful working environment could contribute to an increase in health seeking-behaviours and consequently reduction of maternal mortality.
文摘<b>Background:</b> Good knowledge of diseases’ epidemiology, diagnostic methods and treatment schemes are critical for early detection and effective management of dengue and chikungunya outbreaks. Here, we assessed knowledge and practices regarding dengue and chikungunya among health care workers of some health facilities of Yaoundé and its surroundings. <b>Methods:</b> A descriptive cross-sectional study was conducted from January to August 2020 in 35 health facilities of Yaoundé and its surroundings. Structured pre-tested questionnaire including 11 questions was used to assess healthcare workers’ (i) knowledge on dengue and chikungunya (pathogen, mode of transmission, signs and symptoms) and, (ii) management practices (diagnosis, prevention and treatment measures). Face to face interviews were conducted with healthcare workers including medical doctors, nurses, medical assistants and laboratory assistants. <b>Findings:</b> A total of 232 healthcare workers were interviewed. Despite that 40.95% (n = 95) of respondents had heard about dengue and chikungunya, only 4.74% (n = 11) of them were aware of the fact that both diseases are caused by virus. Also, 9 out of 232 (3.88%) knew that these diseases were transmitted by the bite of an Aedes mosquito. About 15.52% (n = 36) were able to give at least 1 clinical symptom related to dengue and chikungunya. The main diagnostic method they knew was Polymerase Chain Reaction (3.02%;n = 7). For prevention, many participants indicated vector control by using insecticide treated bednets or destroying potential breeding sites (14.23%;n = 33). Only few participants 3.88% (n = 9) knew how to manage dengue or chikungunya cases. To improve the management of both diseases, a significant proportion of healthcare workers (37.93%;n = 88) reported being in need of additional training. <b>Conclusion:</b> The poor knowledge regarding dengue and chikungunya among healthcare workers in Yaoundé and its neighbourhood raises the need to intensify sensitization campaigns and training activities towards this personnel.
文摘<b><span style="font-family:Verdana;">Background:</span></b></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> Understanding the biology of </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> malaria vector species is essential to planning effective and sustainable malaria control strategies in endemic countries. This study reported the implication of </span><i><span style="font-family:Verdana;">Anopheles leesoni </span></i><span style="font-family:Verdana;">in malaria transmission in Cameroon, Central Africa. </span><b><span style="font-family:Verdana;">Methods:</span></b><i> </i><span style="font-family:Verdana;">Mosquitoes were collected in three localities from May 2015 to March 2018 using electric aspirators and Centers for Disease Control light traps (CDC-LT). </span><i><span style="font-family:Verdana;">Anopheles funestus</span></i> <i><span style="font-family:Verdana;">sensu lato</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">.) mosquitoes were identified as species using polymerase chain reaction assay (PCR). Furthermore, </span><i><span style="font-family:Verdana;">Plasmodium falciparum</span></i><span style="font-family:Verdana;"> infection status was determined using the enzyme-linked</span></span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">immunosorbent assay (ELISA) method. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A total of 12,744 </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> mosquitoes were collected by electric aspirator (N = 4844) and CDC-LT (N = 7900). </span><i><span style="font-family:Verdana;">Anopheles funestus</span></i><span> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. (86.95%)</span><i> </i></span><span style="font-family:Verdana;">was the major species and the main malaria vector in rural savannah and rural forest sites followed by </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">. </span><i><span style="font-family:Verdana;">gambiae</span></i><span> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. (13.05%)</span></span><span style="font-family:Verdana;"> whereas</span><span><span style="font-family:Verdana;"> in urban areas, </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> gambiae</span></i> <i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">l</span></i><span style="font-family:Verdana;">. was</span></span><span style="font-family:Verdana;"> by far the most abundant representing 91.45% of </span><i><span style="font-family:Verdana;">Anopheles</span></i><span style="font-family:Verdana;"> mosquitoes collected. Two members of the </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus</span></i><span style="font-family:Verdana;"> group were identified among 1389 analysed by PCR: 1307 </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus sensu stricto</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.)</span><i> </i><span style="font-family:Verdana;">(94.10%) and 82 </span><i><span style="font-family:Verdana;">A</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i><span style="font-family:Verdana;"> (5.9%). </span><i><span style="font-family:Verdana;">Plasmodium falciparum </span></i><span style="font-family:Verdana;">infection rate was 21.04% in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus </span></i></span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i><span style="font-family:Verdana;">. For the first time, </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i> </span><span style="font-family:Verdana;">was found positive for </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i><span style="font-family:Verdana;"> (infection rate: 10.98%)</span></span><span style="font-family:Verdana;"> in Cameroon. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">A very high </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i></span><span style="font-family:Verdana;"> infection rate was observed in this study in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> funestus s</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;">s</span></i></span><span style="font-family:Verdana;">., highlighting its high implication in malaria transmission in Cameroon. Furthermore, the detection of </span><i><span style="font-family:Verdana;">P</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> falciparum</span></i></span><span style="font-family:Verdana;"> infection in </span><i><span style="font-family:Verdana;">A</span></i><span><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> leesoni</span></i></span><span style="font-family:Verdana;"> calls for more attention towards this neglected vector species.
文摘<strong>Background: </strong><span><span><span><span>With the rapid expansion of insecticide resistance limiting the effectiveness of insecticide-based vector control interventions, integrated control strategies associating larviciding could be appropriate to improve current control efforts. The present experimental study assesses laboratory and field efficacy of the larvicide </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> on <i>Anopheline</i> and <i>Culicine</i> larval stages in Yaoundé. <strong>Methods:</strong> The effect of the larvicide </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G,</span></span></span></span><span><span><span><span> a combination of <i>Bacillus</i><span> <i>thuringiensis</i> var. <i>israelensis</i> </span>(<i>Bti</i>) </span></span></span></span><span><span><span><span>and <i>Bacillus</i> <i>sphaericus</i> (<i>Bs</i>),</span></span></span></span><span><span><span><span> on larval development was assessed during both laboratory and open field trial experiments. Laboratory experiments permitted the evaluation of five different concentrations with four replicates/experiments. Laboratory experiments were conducted with <i>Anopheles</i> <i>coluzzii</i> “Ngousso” and <i>Culex</i> <i>quinquefasciatus</i> laboratory strains. Open field trials were conducted using </span></span></span></span><span><span><span><span>sixteen plastic containers with a diameter of 0.31 m buried in an array of four rows with 4 containers each. Distance between rows and between containers in a row was 1 meter. This experiment permitted to </span></span></span></span><span><span><span><span>test the effect of the microbial larvicide </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> under operational application conditions on field mosquito populations. <strong>Results:</strong> <span>The time to induce 100% mortality after exposure to serial concentrations of the larvicide varied according to the dose from 4 - 12 hours for <i>An.</i> <i>coluzzii</i> and 6 - 9 hours for <i>Cx.</i> <i>quinquefasciatus</i> in laboratory experiments. Measurements of the</span> residual activity indicated that all </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> concentrations were still active after 35 days and killed 86</span></span></span></span><span><span><span><span>% </span></span></span></span><span><span><span><span>-</span></span></span></span><span><span><span><span> </span></span></span></span><span><span><span><span>100% of larvae. Lethal dose of </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> killing 50% of larvae was estimated at 5.24 × 10<sup>-8</sup> mg/m<sup>2</sup> for <i>An.</i> <i>coluzzii</i> and 1.25 × 10<sup>-8</sup> mg/m<sup>2</sup> for <i>Cx.</i> <i>quinquefasciatus</i>. The lethal concentration inducing 95% mortality was estimated at 3.13 × 10<sup>-7</sup> mg/m<sup>2</sup> for <i>An.</i> <i>coluzzii</i> and 2.5 × 10<sup>-8</sup> <span>mg/m<sup>2</sup> for <i>Cx.</i> <i>quinquefasciatus</i>. Open field trials tests indicated that </span>sub-lethal concentrations of </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> successfully killed 100% <i>An.</i> <i>gambiae</i> s.l. larvae within 24 hours, while with <i>Culex</i> spp. larvae, 100% mortality was recorded after 48 hours post-treatment. Natural recolonization of water containers by larvae was recorded between 3 and 6 days respectively after the treatment with sublethal doses. Late instar larvae were recorded 5 and 6 days after treatment. When the jars were treated with reference dosage or supra doses of </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G,</span></span></span></span><span><span><span><span> recolonization of water containers was observed six days after treatments. No pupae of both species were found 6 and 7 days post-treatment. <strong>Conclusions:</strong> The study indicated high efficacy of the microbial larvicide </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> against <i>Anopheline</i> and <i>Culex</i> larvae. Microbial larvicides such as </span></span></span></span><span><span><span><span>VectoMax</span></span></span></span><span><span><span><span style="color:#111111;font-family:Roboto, sans-serif;font-size:16px;white-space:normal;background-color:#FFFFFF;"><sup>®</sup></span></span></span></span><span><span><span><span>G</span></span></span></span><span><span><span><span> could be appropriate for controlling mosquito population particularly in areas experiencing high insecticide resistance or outdoor biting mosquitoes.</span></span></span></span>
文摘研究院长负责制的实施北景、内容及对人员表现的影响。选择社会经济水平不同的B县(较富裕)和C县(较贫困)两个县进行调查,以召开专题小组讨论(focus group discussion)和知情者访谈(key informant interview)的定性调查方法为主,结合医院收支、诊疗等方面的定量数据进行分析。结果提示院长负责制受到院长、卫技人员的普遍拥护,调动院长与职工的工作积极性。实行院长负责制期间,两个县医院的医疗活动、经济创收、满意度与人员培训方面均有所改善。分析“放权”政策的局限性以及改善人员表现应采取的措施。讨论实行并完善院长负责制要正确处理扩大自主权和强化束约力的辩证关系和正确认识与理解新形势下的院长负责制等问题。