By using chemical mutagenesis, ‘Huayu 40’, a normal-oleic(NO) large-seed high yielding peanut cultivar with wide adaptability was transformed into mutants with desirable high-oleic(HO) quality traits, comparable pro...By using chemical mutagenesis, ‘Huayu 40’, a normal-oleic(NO) large-seed high yielding peanut cultivar with wide adaptability was transformed into mutants with desirable high-oleic(HO) quality traits, comparable productivity and adaptability. Of the 3 chemical treatments, viz. 5 mM ethyl methane sulfonate(EMS), 25 mM EMS and 15 mM sodium azide(NaN_3), only NaN_3 produced HO M_2 mutant plants. High oleate phenotype was initially identified by near infrared spectroscopy(NIRS) screening, and was further confirmed by gas spectrometry(GC) analysis. Sequence analysis showed that HO plant had a 448 G>A mutation in FAD2 A and a 441_442 insA mutation in FAD2 B, respectively. Although the direct use of high yielding and stress resistant cultivars/lines to induce quality mutations might be a judicious choice to accelerate breeding, our results showed that chemical mutagenesis could quickly create HO peanut materials and expand the genetic bases of HO peanuts.展开更多
基金financial support from the China Agricultural Research System (CARS-13)the Agricultural Scientifc and Technological Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2016-02, CXGC2018E21)+3 种基金the Yantai Science and Technology Plan Project (2018ZDCX)the Shandong Key Research and Development Program (2018GNC110027)Taishan Industry Leading Talents Special Fund and Key Laboratory of Agro-Products Processing, Ministry of AgricultureRural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (NCPJG 2018-001)
文摘By using chemical mutagenesis, ‘Huayu 40’, a normal-oleic(NO) large-seed high yielding peanut cultivar with wide adaptability was transformed into mutants with desirable high-oleic(HO) quality traits, comparable productivity and adaptability. Of the 3 chemical treatments, viz. 5 mM ethyl methane sulfonate(EMS), 25 mM EMS and 15 mM sodium azide(NaN_3), only NaN_3 produced HO M_2 mutant plants. High oleate phenotype was initially identified by near infrared spectroscopy(NIRS) screening, and was further confirmed by gas spectrometry(GC) analysis. Sequence analysis showed that HO plant had a 448 G>A mutation in FAD2 A and a 441_442 insA mutation in FAD2 B, respectively. Although the direct use of high yielding and stress resistant cultivars/lines to induce quality mutations might be a judicious choice to accelerate breeding, our results showed that chemical mutagenesis could quickly create HO peanut materials and expand the genetic bases of HO peanuts.
