Mitochondrial oxaloacetate transporter protein encoded by OAT gene transports oxaloacetate from cytoplasm into mitochondria. To investigate the primary effects of OAT gene on relative metabolism in Aspergillus niger, ...Mitochondrial oxaloacetate transporter protein encoded by OAT gene transports oxaloacetate from cytoplasm into mitochondria. To investigate the primary effects of OAT gene on relative metabolism in Aspergillus niger, a oat-deleted mutant was derived from wild-type A. niger ATCC1015 using the double-crossover chromosome replacement technique. Then batch fermentation was performed to evaluate the mutant. Compared with the wild type, the mutant showed lower organic acids production, with the experimental data that the production of pyruvate and 2-ketoglutarate decreased by 31.6% and 35.7%, respectively, and by contrast, the mutant showed higher glycerol formation. The results suggest that OAT gene plays significant roles on metabolism in A. niger.展开更多
Cancerous inhibitor of protein phosphatase 2A(CIP2A),an endogenous protein phosphatase 2A(PP2A)inhibitor,has been identified as an oncoprotein in promoting cancer initiation and progression of several types of cancer....Cancerous inhibitor of protein phosphatase 2A(CIP2A),an endogenous protein phosphatase 2A(PP2A)inhibitor,has been identified as an oncoprotein in promoting cancer initiation and progression of several types of cancer.However,the expression and the role played by CIP2A in the pathogenesis of multiple myeloma(MM)remain unclear.In this study,we showed that CIP2A was overexpressed in human MM cell lines and MM patients’bone marrow tissues.Clinicopathologic analysis showed that CIP2A expression was significantly correlated with clinical stage and percent of plasma cells in bone marrow.Kaplan–Meier analysis revealed that patients with high CIP2A expression presented with poorer overall survival rates than those with low CIP2A expression.Moreover,CIP2A knockdown in MM cells resulted in attenuated proliferative abilities.In addition,CIP2A depletion sensitizes dexamethasone(Dex)-resistant cells to Dex.The effect of CIP2A on proliferation and Dex therapy was mediated by the inhibition of PP2A,which in turn activated Akt.In vivo studies confirmed that CIP2A regulated MM tumorigenesis and the phosphorylation of Akt.Taken together,our results suggest that CIP2A oncoprotein plays an important role in MM progression and could serve as a prognosis marker and a novel therapeutic target for the treatment of patients with MM.展开更多
文摘Mitochondrial oxaloacetate transporter protein encoded by OAT gene transports oxaloacetate from cytoplasm into mitochondria. To investigate the primary effects of OAT gene on relative metabolism in Aspergillus niger, a oat-deleted mutant was derived from wild-type A. niger ATCC1015 using the double-crossover chromosome replacement technique. Then batch fermentation was performed to evaluate the mutant. Compared with the wild type, the mutant showed lower organic acids production, with the experimental data that the production of pyruvate and 2-ketoglutarate decreased by 31.6% and 35.7%, respectively, and by contrast, the mutant showed higher glycerol formation. The results suggest that OAT gene plays significant roles on metabolism in A. niger.
基金This work was supported by grants from the National Natural Sciences Foundation of China(no.81400157)the Natural Science Foundation of Hubei Province(no.2016CFB528)+3 种基金the Foundation of Health and Family planning Commission of Hubei Province(no.WJ2017F065)the Faculty Development Grants from Hubei University of Medicine(nos 2014QDJZR08 and 2015QDJZR16)the Foundation of Hubei University of Medicine(no.FDFR201605)the Foundation for Innovative Research Team of Hubei University of Medicine(no.2014CXX05).
文摘Cancerous inhibitor of protein phosphatase 2A(CIP2A),an endogenous protein phosphatase 2A(PP2A)inhibitor,has been identified as an oncoprotein in promoting cancer initiation and progression of several types of cancer.However,the expression and the role played by CIP2A in the pathogenesis of multiple myeloma(MM)remain unclear.In this study,we showed that CIP2A was overexpressed in human MM cell lines and MM patients’bone marrow tissues.Clinicopathologic analysis showed that CIP2A expression was significantly correlated with clinical stage and percent of plasma cells in bone marrow.Kaplan–Meier analysis revealed that patients with high CIP2A expression presented with poorer overall survival rates than those with low CIP2A expression.Moreover,CIP2A knockdown in MM cells resulted in attenuated proliferative abilities.In addition,CIP2A depletion sensitizes dexamethasone(Dex)-resistant cells to Dex.The effect of CIP2A on proliferation and Dex therapy was mediated by the inhibition of PP2A,which in turn activated Akt.In vivo studies confirmed that CIP2A regulated MM tumorigenesis and the phosphorylation of Akt.Taken together,our results suggest that CIP2A oncoprotein plays an important role in MM progression and could serve as a prognosis marker and a novel therapeutic target for the treatment of patients with MM.
