In order to increase the yield of polysaccharides from Porphyridium cruentum and the cell biomass, we investigated several important cultural conditions such as nutrient-salts, light irradiance as well as growth condi...In order to increase the yield of polysaccharides from Porphyridium cruentum and the cell biomass, we investigated several important cultural conditions such as nutrient-salts, light irradiance as well as growth conditions in the flat plate photobioreacturs (FPPBR) with different light-path. We have found that the optimal (OCM II ) was a superior medium for extracellular polysaccharide production than the f/2 and Koch medium, and the polysaccharide production with OCM II was increased by 1.79 times and 1.62 times compared with that of f/2 and Koch medium; the yields of polysaccharides were also improved by optimizing the light intensity and irradiation length Furthermore, we significantly enhanced the yields of both the biomass and extracellular polysaccharidc using a small light-path photobioreactor, and about 2.1 times increase of polysaccharide production was observed in FPPBR-30 than in FPPBR-100. Overall, the process for polysaccharides production was improved and this would facilitate further studies on such polysaccharidcs such as their antiviral and antitumor activities.展开更多
文摘In order to increase the yield of polysaccharides from Porphyridium cruentum and the cell biomass, we investigated several important cultural conditions such as nutrient-salts, light irradiance as well as growth conditions in the flat plate photobioreacturs (FPPBR) with different light-path. We have found that the optimal (OCM II ) was a superior medium for extracellular polysaccharide production than the f/2 and Koch medium, and the polysaccharide production with OCM II was increased by 1.79 times and 1.62 times compared with that of f/2 and Koch medium; the yields of polysaccharides were also improved by optimizing the light intensity and irradiation length Furthermore, we significantly enhanced the yields of both the biomass and extracellular polysaccharidc using a small light-path photobioreactor, and about 2.1 times increase of polysaccharide production was observed in FPPBR-30 than in FPPBR-100. Overall, the process for polysaccharides production was improved and this would facilitate further studies on such polysaccharidcs such as their antiviral and antitumor activities.