Most of marine sponges harbor dense and diverse microbial communities of bioactivity importance. Four Gram positive bacterial cultures (HA-21, HA-68, HA- MS-105 and HA-MS-119) were isolated from the sponge Amphimedon ...Most of marine sponges harbor dense and diverse microbial communities of bioactivity importance. Four Gram positive bacterial cultures (HA-21, HA-68, HA- MS-105 and HA-MS-119) were isolated from the sponge Amphimedon ochracea, collected from the Red Sea coast of Egypt. Bacterial species were identified based on the phylogenetic analysis of the nucleotide sequences of their 16S rDNA genes. The Sequences similarity values of 98% - 100% to other strains in the NCBI database showed strong similarities with the 16S rDNA genes of firmicutes (Bacillus sp.). The four bacterial species were submitted to the GenBank database and had accession numbers of: HA-21 [JQ-768238];HA-68 [JQ751264];HA-MS-105 [JQ768239];HAMS-119 [JQ768240]. The cytotoxic activities of the bacterial isolates were tested against three established human cancer cell lines;HepG2 (hepatocellular carcinoma), HCT (colon carcinoma) and MCF-7 (breast carcinoma). The inhibitory effect on these cell lines, measured by MTT cell assay protocol, revealed promising cytotoxic activity of the four isolates (IC50 values (μg/mL) were: HA-21: 13.2, 9.3 and 12.2;HA-68: 10.42, 4.3 and 5.5;HA-MS-105: 46.9, 28.6 and 21.3;HAMS-119: 10.42, 6.3 and 22.1;respectively). The recovery of bacterial strains with cytotoxic activity suggests that marine invertebrates remain a rich source for the isolation of culturable isolates capable of producing novel bioactive secondary metabolites.展开更多
The present study focused on water quality assessment of 14 hotspot locations in the Gulf of Suez by measuring the physicochemical parameters seasonally during 2016. The results of investigated area revealed that, the...The present study focused on water quality assessment of 14 hotspot locations in the Gulf of Suez by measuring the physicochemical parameters seasonally during 2016. The results of investigated area revealed that, the annual mean range of water was: temperature (21.91°C - 29.22°C), pH (7.64 - 7.78), salinity (38.71‰ - 42.74‰), dissolved oxygen (6.09 - 8.78 mgO2/l,) oxidizable organic matter (1.4 - 5.4 mg/l), biological oxygen demand (1.14 - 3.94 mgO2/l), total suspended solids (18.56 - 37.69 mg/l), ammonia (13.51 - 494.41 μg/l), nitrite (1.261 - 151.76 μg/l), nitrate (7.11 - 487.85), dissolved inorganic phosphate (2.22 - 53.26) and silicate (19.83 - 347.61 μg/l). The N:P ratio fluctuated between 4.21 and 1214.61 with the main value of 81.16 indicating that the different sites in the northern part of the Gulf of Suez are P-limited. Based on the Principal Component Analysis Data, the stations locating in the Northern and Southern side of the Gulf of Suez are relatively good water quality;meanwhile, water quality of the other stations locating in the northern side of the Gulf of Suez is found slightly polluted to a different degree co-incided with an increase in the human activities in each of these locations.展开更多
Thirty five Sediment samples were collected from three Northern Egyptian Lakes namely El Burullus, El Manzala and El Bardawil to evaluate for the first time the concentrations of poorly studied technology-critical ele...Thirty five Sediment samples were collected from three Northern Egyptian Lakes namely El Burullus, El Manzala and El Bardawil to evaluate for the first time the concentrations of poorly studied technology-critical elements, namely Ge, Zr, Mo, Sn, Sb, Hf, Ta, W and Ti, and how that may be linked to local water quality parameters and other geochemical factors. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to determine element’s concentration. The results were compared with Upper Continental Crust (UCC) and Nile sediment (ANS). Lake sediments generally have relatively low contents of most elements. The average concentration of Zr, Hf, Ta and W are lower than UCC and ANS in three Lakes. El Bardawil Lake attained the lowest average concentration for all elements except Mo. Correlation analysis revealed that water quality and geochemical properties did not play any important role affecting the metal distribution and the studied metals are generally not affected by the complex lacustrine system of these lakes and their spatial distribution still not affected by the different anthropogenic activates.展开更多
文摘Most of marine sponges harbor dense and diverse microbial communities of bioactivity importance. Four Gram positive bacterial cultures (HA-21, HA-68, HA- MS-105 and HA-MS-119) were isolated from the sponge Amphimedon ochracea, collected from the Red Sea coast of Egypt. Bacterial species were identified based on the phylogenetic analysis of the nucleotide sequences of their 16S rDNA genes. The Sequences similarity values of 98% - 100% to other strains in the NCBI database showed strong similarities with the 16S rDNA genes of firmicutes (Bacillus sp.). The four bacterial species were submitted to the GenBank database and had accession numbers of: HA-21 [JQ-768238];HA-68 [JQ751264];HA-MS-105 [JQ768239];HAMS-119 [JQ768240]. The cytotoxic activities of the bacterial isolates were tested against three established human cancer cell lines;HepG2 (hepatocellular carcinoma), HCT (colon carcinoma) and MCF-7 (breast carcinoma). The inhibitory effect on these cell lines, measured by MTT cell assay protocol, revealed promising cytotoxic activity of the four isolates (IC50 values (μg/mL) were: HA-21: 13.2, 9.3 and 12.2;HA-68: 10.42, 4.3 and 5.5;HA-MS-105: 46.9, 28.6 and 21.3;HAMS-119: 10.42, 6.3 and 22.1;respectively). The recovery of bacterial strains with cytotoxic activity suggests that marine invertebrates remain a rich source for the isolation of culturable isolates capable of producing novel bioactive secondary metabolites.
文摘The present study focused on water quality assessment of 14 hotspot locations in the Gulf of Suez by measuring the physicochemical parameters seasonally during 2016. The results of investigated area revealed that, the annual mean range of water was: temperature (21.91°C - 29.22°C), pH (7.64 - 7.78), salinity (38.71‰ - 42.74‰), dissolved oxygen (6.09 - 8.78 mgO2/l,) oxidizable organic matter (1.4 - 5.4 mg/l), biological oxygen demand (1.14 - 3.94 mgO2/l), total suspended solids (18.56 - 37.69 mg/l), ammonia (13.51 - 494.41 μg/l), nitrite (1.261 - 151.76 μg/l), nitrate (7.11 - 487.85), dissolved inorganic phosphate (2.22 - 53.26) and silicate (19.83 - 347.61 μg/l). The N:P ratio fluctuated between 4.21 and 1214.61 with the main value of 81.16 indicating that the different sites in the northern part of the Gulf of Suez are P-limited. Based on the Principal Component Analysis Data, the stations locating in the Northern and Southern side of the Gulf of Suez are relatively good water quality;meanwhile, water quality of the other stations locating in the northern side of the Gulf of Suez is found slightly polluted to a different degree co-incided with an increase in the human activities in each of these locations.
文摘Thirty five Sediment samples were collected from three Northern Egyptian Lakes namely El Burullus, El Manzala and El Bardawil to evaluate for the first time the concentrations of poorly studied technology-critical elements, namely Ge, Zr, Mo, Sn, Sb, Hf, Ta, W and Ti, and how that may be linked to local water quality parameters and other geochemical factors. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to determine element’s concentration. The results were compared with Upper Continental Crust (UCC) and Nile sediment (ANS). Lake sediments generally have relatively low contents of most elements. The average concentration of Zr, Hf, Ta and W are lower than UCC and ANS in three Lakes. El Bardawil Lake attained the lowest average concentration for all elements except Mo. Correlation analysis revealed that water quality and geochemical properties did not play any important role affecting the metal distribution and the studied metals are generally not affected by the complex lacustrine system of these lakes and their spatial distribution still not affected by the different anthropogenic activates.
