Allelopathic growth inhibition of Heterosigma akashiwo by the three Ulva spcieces(Ulva Pertusa,Ulva Linza,Enteromorpha intestinalis) under laboratory conditions

Allelopathic effects of several concentrations of fresh tissue,dry powder and dry tissue of three bloom-forming green macroalgae Ulva pertusa,Ulva linza and Enteromopha intestinalis on the red tide microalga Heterosigma akashiwo were evaluated in microcosms systems.The effects of macroalgae culture medium filtrate were investigated on H.akashiwo using initial or semi-continuous filtrate addition.Preliminary studies on the algicidal effects of one aqueous and four organic solvent extracts from the macroalgae on the microalga were carried out to confirm the existence of allelochemicals in the tissue of these macroalgae.The dry powder of U.pertusa was extracted with methanol,and the methanol extracts were partitioned to petroleum ether phase,ethyl acetate phase,butanol phase and distilled phase by liquid-liquid fractionation.The bioassays of the activity of every fraction were carried out on H.akashiwo.The resultant microcosms assay showed that the growth of H.akashiwo was strongly inhibited by using fresh tissues,dry powder or dry tissue of these three macroalgae,while aqueous and methanol extracts of both macroalgae had strong inhibitory effects on the growth of H.akashiwo,and the EC50 values for methanol extract of U.pertusa,U.linza or E.intestinalis were 0.016,0.028× 10-12 or 0.033× 10-12,respectively.While the other three organic solvent extracts (acetone,ether and chloroform) had no apparent effect on its growth,this suggests that the allelochemicals from these three maeroalgae had relatively high polarities.The activity of petroleum ether phase,ethyl acetate phase,butanol phase and distilled phase of U.pertusa methanol extract was carried out on H.akashiwo indicating that petroleum ether phase and ethyl acetate phase had stronger algicidal effect on H.akashiwo.The inhibition effect of the ethyl acetate phase was not as strong as that of petroleum ether phase,and effective concentration of petroleum ether phase was 17 mg/L for H.akashiwo.However,no significant algicidal effects were observed on the butanol phase and distilled water phase.These three macroalgae's culture medium filtrate exhibited no apparent growth inhibitory effect on the microalga under initial filtrate addition whereas the growth of H.akashiwo was significantly inhibited under semi-continuous filtrate addition,which suggests that continuous release of small quantities of rapidly degradable allelochemicals from the fresh tissue of both macroalgae was effective in inhibiting the growth of H.akashiwo.

Identification of the toxic compounds produced by Sargassum thunbergii to red tide microalgae

The inhibitory effects of methanol extracts from the tissues of three macroalgal species on the growths of three marine red tide microalgae were assessed under laboratory conditions.Extracts of Sargassum thunbergii(Mertens ex Roth) Kuntz tissue had stronger inhibitory effects than those of either Sargassum pallidum(Turner) C.Agardh or Sargassum kjellmanianum Yendo on the growths of Heterosigma akashiwo(Hada) Hada,Skeletonema costatum(Grev.) Grev,and Prorocentrum micans Ehrenberg.Methanol extracts of S.thunbergii were further divided into petroleum ether,ethyl acetate,butanol,and distilled water phases by liquid-liquid fractionation.The petroleum ether and ethyl acetate fractions had strong algicidal effects on the microalgae.Gas chromatography-mass spectrometry analyses of these two phases identified nine fatty acids,most of which were unsaturated fatty acids.In addition,pure compounds of four of the nine unsaturated fatty acids had effective concentrations below 5 mg/L.Therefore,unsaturated fatty acids are a component of the allelochemicals in S.thunbergii tissue.

Experimental Study on the Interspecific Interactions Between the Two Bloom-Forming Algal Species and the Rotifer Brachionus plicatilis

The interspecific interactions between the rotifer Brachionus plicatilis and two harmful algal blooms(HAB) species were investigated experimentally by single culture method. B. plicatilis population and the growth of the two algae were compared at different algal cell densities. The results demonstrated that the B. plicatilis obtained sufficient nutrition from Prorocentrum donghaiense to support net population increase. With exposure to 2.5×104 cells mL-1 of P. donghaiense,the number of B. plicatilis increased faster than it did when exposed to other four algal densities(5,10,15 and 20 ×104 cells mL-1),and the increase rate of B. plicatilis population(r) at this algal density was 0.104 ± 0.015 r d-1. Cell densities of P. donghaiense decreased due to the grazing of B. plicatilis. In contrast,Heterosigma akashiwo had an adverse effect on B. plicatilis population and its growth was largely unaffected by rotifer grazing. In this case,B. plicatilis population decreased and H. akashiwo grew at a rate similar to that of the control.

Comparative Studies on the Allelopathic Effects of Ulva pertusa Kjellml, Corllina pilulifera Postl et Ruprl, and Sargassum thunbergii mertl O. Kuntze on Skeletonema costatum （Grev.）Cleve

In the present study, we evaluated the allelopathic effects of three macroalgae, namely Ulva pertusa Kjellml, Corallina pilufifera Postl et Ruprl, and Sargassum thunbergii Mertl O. Kuntze, on the growth of the microalga Skeletonema costaturn （Grev.） Creve using culture systems in which the algae coexisted. The effects of the macroalgal culture medium filtrate on S. costatum were also investigated. Moreover, isolated co-culture systems were built to confirm the existence of allelochemicals and preclude growth inhibition by direct contact. The coexistence assay data demonstrated that the growth of S. costaturn was strongly inhibited when fresh tissues, dry powder and aqueous extracts were used; the allelochemicals were lethal to S. costatum at relatively higher concentrations. The effects of the macroalgal culture medium filtrate on the microalga showed both species specificity and complexity. The inhibitory effect of fresh macroalgal tissue and culture medium filtrate on the microalga was due to the alleochemicals released by the macroalgae. The results of the present study show that the allelopathic effects of macroalgae on the microalga are complex. The present study could shed light onto the basis of the interaction between macro- and microalgae.

背景匹配的和破坏性的色彩之间的相互作用：利用人类的天敌和虚拟螃蟹实验

破坏性的着色和猎物和背景光斑尺寸之间的匹配之间的相互作用，在两个实验所用的时间由人类“捕食者”找到人工猎物操纵（虚拟蟹变种）对异构背景作为衡量他们的伪装。实验1，在该点及其布局模仿对蟹Carcinus maenas的安排，测试是否高和低对比度的点接触身体的轮廓（破坏性的“边缘”的变种）的人工猎物比更难检测点时，未触及的轮廓（非破坏性的“内在”的变种）对三不同的背景：＇小＇，＇中间＇和＇大＇的光斑尺寸。在实验2中，光斑的大小和位置的范围（边缘＇和＇内＇）对螃蟹的变体不同来确定最有效的组合对中为背景。总共有640名志愿者参加了这些计算机试验，代表着高度的独立复制。所有图案的变种检测比普通的变种非常困难，这表明至少具有一定程度的背景匹配可以提供伪装。实验表明，不同的变种，但不具有相同的尺寸为背景，有类似的或更好的生存与匹配大小的点——表明优化玛伪装不是来自背景匹配的孤独。在实验2中，边破坏形态始终需要更长的时间来找到比他们的非破坏性的同行。边缘破裂，对比的相对影响，和背景/猎物光斑尺寸显然是上下文相关的，突出的猎物隐藏[当前生态61的复杂性（4）：718-728，2015 ]。