Carnosine concentration and expression profiles of carnosine related genes in Mytilus after beta-alanine injection

Carnosine and its analogues are histidine-containing dipeptides(HCDs)playing diverse functions in vertebrates.However,the distribution and the metabolism of carnosine in invertebrates are still unknown.In this study,Mytilus coruscus,a shellfish with important economic value in China,was selected for the investigation of HCD content and the expression profiling of carnosine-related genes in various mussel tissues.Quantification of HCD by amino acids analyzer revealed a low concentration of anserine in muscular tissues in Mytilus,indicating the presence of HCD even in an invertebrate.mRNA expression of five carnosine metabolic-related genes was profiled in various tissues,and the results highlighted the relative higher expression level of these genes in muscular tissues.Considering the fact that beta-alanine supplementation can increase the HCD content in vertebrates,a beta-alanine injection was performed and the changes of HCD concentration and the mRNA expression of carnosine related genes were investigated in five mussel tissues.The results revealed the increase of HCD concentration,as well as the up-regulated expression level of related genes,in tested tissues of beta-alanine injected mussels.Transcriptomic analysis for the whole soft tissue of mussel before and after beta-alanine injection were performed,and 3569 differential expression genes(DEGs)were identified in the beta-alanine injected group when compared to their expression levels in the control.These data indicated the complex eff ects of betaalanine on M.coruscus metabolism,and those DEGs enriched in pathways of cancers,muscle contraction,and tyrosine metabolism highlighted the possible functions of beta-alanine in cell proliferation,sports,and melanogenesis,respectively.

A Method for Sea Surface Wind Field Retrieval from SAR Image Mode Data

To retrieve wind field from SAR images, the development for surface wind field retrieval from SAR images based on the improvement of new inversion model is present. Geophysical Model Functions(GMFs) have been widely applied for wind field retrieval from SAR images. Among them CMOD4 has a good performance under low and moderate wind conditions. Although CMOD5 is developed recently with a more fundamental basis, it has ambiguity of wind speed and a shape gradient of normalized radar cross section under low wind speed condition. This study proposes a method of wind field retrieval from SAR image by combining CMOD5 and CMOD4 Five VV-polarisation RADARSAT2 SAR images are implemented for validation and the retrieval results by a combination method(CMOD5 and CMOD4) together with CMOD4 GMF are compared with QuikSCAT wind data. The root-mean-square error(RMSE) of wind speed is 0.75 m s-1 with correlation coefficient 0.84 using the combination method and the RMSE of wind speed is 1.01 m s-1 with correlation coefficient 0.72 using CMOD4 GMF alone for those cases. The proposed method can be applied to SAR image for avoiding the internal defect in CMOD5 under low wind speed condition.