AIM To assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer asymmetry.METHODS Rats were fed either with normal chow or a lowsodium diet. Tissues wer...AIM To assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer asymmetry.METHODS Rats were fed either with normal chow or a lowsodium diet. Tissues were mounted as flat sheets in a modified, airtight Ussing chamber with oxygen meters in each hemichamber. Mucosal samples from normal diet animals were studied under control conditions, in low-chloride solution and after adding chloride secretion inhibitors and chloride secretagogues. Samples from sodium-deprived rats were studied before and after ouabain addition. In separate experiments, the correlation between short-circuit current and oxygen consumption was analyzed. Finally, hypoxia was induced in one hemichamber to assess the relationship between its oxygen content and the oxygen pressure differencebetween both hemichambers. RESULTS In all studied conditions, oxygen consumption was larger in the serosal hemichamber than in the mucosal one(P = 0.0025 to P < 0.0001). Short-circuit current showed significant correlation with both total oxygen consumption(r = 0.765; P = 0.009) in normoxia and oxygen consumption in the serosal hemichamber(r = 0.754; P = 0.011) during mucosal hypoxia, but not with oxygen consumption in the mucosal hemichamber. When hypoxia was induced in the mucosal hemichamber, an oxygen pressure difference of 13 k Pa with the serosal hemichamber was enough to keep its oxygen content constant. However, when hypoxia was induced in the serosal hemichamber, the oxygen pressure difference with the mucosal hemichamber necessary to keep its oxygen content constant was 40 k Pa(P < 0.0001). CONCLUSION Serosal oxygen supply is more readily available to support short-circuit current. This may be partly due to a rectifying behavior of transepithelial oxygen transfer.展开更多
基金Supported by The Secretaría de Ciencia,Técnica y Posgrado,National University of Cuyo,Mendoza 5500 Argentina to Saraví FD,No.06/J457
文摘AIM To assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer asymmetry.METHODS Rats were fed either with normal chow or a lowsodium diet. Tissues were mounted as flat sheets in a modified, airtight Ussing chamber with oxygen meters in each hemichamber. Mucosal samples from normal diet animals were studied under control conditions, in low-chloride solution and after adding chloride secretion inhibitors and chloride secretagogues. Samples from sodium-deprived rats were studied before and after ouabain addition. In separate experiments, the correlation between short-circuit current and oxygen consumption was analyzed. Finally, hypoxia was induced in one hemichamber to assess the relationship between its oxygen content and the oxygen pressure differencebetween both hemichambers. RESULTS In all studied conditions, oxygen consumption was larger in the serosal hemichamber than in the mucosal one(P = 0.0025 to P < 0.0001). Short-circuit current showed significant correlation with both total oxygen consumption(r = 0.765; P = 0.009) in normoxia and oxygen consumption in the serosal hemichamber(r = 0.754; P = 0.011) during mucosal hypoxia, but not with oxygen consumption in the mucosal hemichamber. When hypoxia was induced in the mucosal hemichamber, an oxygen pressure difference of 13 k Pa with the serosal hemichamber was enough to keep its oxygen content constant. However, when hypoxia was induced in the serosal hemichamber, the oxygen pressure difference with the mucosal hemichamber necessary to keep its oxygen content constant was 40 k Pa(P < 0.0001). CONCLUSION Serosal oxygen supply is more readily available to support short-circuit current. This may be partly due to a rectifying behavior of transepithelial oxygen transfer.
