Fabrication of trace elements incorporated apatite coating could combine the ions’ pharmaceutical effect into the materials. In this study, strontium, silicon, and fluoride ions have been incorporated into apatite co...Fabrication of trace elements incorporated apatite coating could combine the ions’ pharmaceutical effect into the materials. In this study, strontium, silicon, and fluoride ions have been incorporated into apatite coatings through a biomineralization method, which mimics an in vitro mineralization process. The surface composition is tested with X-ray diffraction and X-ray photoelectron spectroscopy, and the surface morphology is characterized with scanning electron microscopy. Compared with pure hydroxyapatite coating, the strontium, silicon, and fluoride substituted apatite coatings show different morphology as spherical, needle-like, and nano-flake-like, individually. The crystal size of these biomimetic hydroxyapatite coatings decreased after ion substitution. The results of the analysis of surface composition present the ion substitutions are increased with the increasing of ion concentrations in the soaking solution. That means the ion incorporation into the apatite structure based on the biomineralization method could not only vary the ion content in but also change the morphology of the apatite coatings. Herein, the role of ion substitution is considered from the point of view of materials science at the micro structural and surface chemistry levels.展开更多
Regenerative bone implants promote new bone formation and ideally degrade simultaneously to osteogenesis.Although clinically established calcium phosphate bone grafts provide excellent osseointegration and osteoconduc...Regenerative bone implants promote new bone formation and ideally degrade simultaneously to osteogenesis.Although clinically established calcium phosphate bone grafts provide excellent osseointegration and osteoconductive efficacy,they are limited in terms of bioresorption.Magnesium phosphate(MP)based ceramics are a promising alternative,because they are biocompatible,mechanically extremely stable,and degrade much faster than calcium phosphates under physiological conditions.Bioresorption of an implant material can include both chemical dissolution as well as cellular resorption.We investigated the bioresorption of 3D powder printed struvite and newberyite based MP ceramics in vitro by a direct human osteoclast culture approach.The osteoclast response and cellular resorption was evaluated by means of fluorescence and TRAP staining,determination of osteoclast activities(CA II and TRAP),SEM imaging as well as by quantification of the ion release during cell culture.Furthermore,the bioactivity of the materials was investigated via SBF immersion,whereas hydroxyapatite precipitates were analyzed by SEM and EDX measurements.This bioactive coating was resorbed by osteoclasts.In contrast,only chemical dissolution contributed to bioresorption of MP,while no cellular resorption of the materials was observed.Based on our results,we expect an increased bone regeneration effect of MP compared to calcium phosphate based bone grafts and complete chemical degradation within a maximum of 1.5-3.1 years.展开更多
The authors regret the reversal of the order of first and last names in the original article.The correct order of the authors’first and last names are Eugen Gefel,Claus Moseke,Anna-Maria Schmitt,Niklas Dümmler,P...The authors regret the reversal of the order of first and last names in the original article.The correct order of the authors’first and last names are Eugen Gefel,Claus Moseke,Anna-Maria Schmitt,Niklas Dümmler,Philipp Stahlhut,Andrea Ewald,Andrea Meyer-Lindenberg,Elke Vorndran.展开更多
基金This work was supported by BIOMATCELL,VINN Ex-cellence Center of Biomaterials and Cell Therapy.
文摘Fabrication of trace elements incorporated apatite coating could combine the ions’ pharmaceutical effect into the materials. In this study, strontium, silicon, and fluoride ions have been incorporated into apatite coatings through a biomineralization method, which mimics an in vitro mineralization process. The surface composition is tested with X-ray diffraction and X-ray photoelectron spectroscopy, and the surface morphology is characterized with scanning electron microscopy. Compared with pure hydroxyapatite coating, the strontium, silicon, and fluoride substituted apatite coatings show different morphology as spherical, needle-like, and nano-flake-like, individually. The crystal size of these biomimetic hydroxyapatite coatings decreased after ion substitution. The results of the analysis of surface composition present the ion substitutions are increased with the increasing of ion concentrations in the soaking solution. That means the ion incorporation into the apatite structure based on the biomineralization method could not only vary the ion content in but also change the morphology of the apatite coatings. Herein, the role of ion substitution is considered from the point of view of materials science at the micro structural and surface chemistry levels.
基金funded by the Deutsche Forschungsgemeinschaft,grant number 417069397,as a collaboration project between the Ludwig Maximilians University Munich and the University Clinic of Würzburgthe DFG for funding the crossbeam scanning electron microscope Zeiss CB 340(INST 105022/58-1 FUGG)within the DFG State Major Instrumentation Programme。
文摘Regenerative bone implants promote new bone formation and ideally degrade simultaneously to osteogenesis.Although clinically established calcium phosphate bone grafts provide excellent osseointegration and osteoconductive efficacy,they are limited in terms of bioresorption.Magnesium phosphate(MP)based ceramics are a promising alternative,because they are biocompatible,mechanically extremely stable,and degrade much faster than calcium phosphates under physiological conditions.Bioresorption of an implant material can include both chemical dissolution as well as cellular resorption.We investigated the bioresorption of 3D powder printed struvite and newberyite based MP ceramics in vitro by a direct human osteoclast culture approach.The osteoclast response and cellular resorption was evaluated by means of fluorescence and TRAP staining,determination of osteoclast activities(CA II and TRAP),SEM imaging as well as by quantification of the ion release during cell culture.Furthermore,the bioactivity of the materials was investigated via SBF immersion,whereas hydroxyapatite precipitates were analyzed by SEM and EDX measurements.This bioactive coating was resorbed by osteoclasts.In contrast,only chemical dissolution contributed to bioresorption of MP,while no cellular resorption of the materials was observed.Based on our results,we expect an increased bone regeneration effect of MP compared to calcium phosphate based bone grafts and complete chemical degradation within a maximum of 1.5-3.1 years.
文摘The authors regret the reversal of the order of first and last names in the original article.The correct order of the authors’first and last names are Eugen Gefel,Claus Moseke,Anna-Maria Schmitt,Niklas Dümmler,Philipp Stahlhut,Andrea Ewald,Andrea Meyer-Lindenberg,Elke Vorndran.
