Dynamic expression of rat heat shock protein gp96 and its gene during development of hepatocellular carcinoma

BACKGROUND: Hepatocellular carcinoma (HCC) is characterized by multicause, obvious multistage and multifocal processes of tumor progression. The development of HCC is related intimately to overexpression and signal transduction of many cellular factors. This study was undertaken to investigate the dynamic expression and alteration of heat shock protein (HSP) gp96 along with its gene during HCC development. METHODS: A rat model of hepatoma induced with 2-fluorenylacetamide (2-FAA, 0.05%) was established in male Sprague-Dawley rats. Total RNA and pathological changes were observed during hepatocarcinogenesis. Total RNAs were transcribed into cDNA by reverse transcription and the gene fragment of gp96 was amplified by nested RT-PCR. The gp96 expression in rat liver tissues was semi-quantitatively analyzed by immunohistochemistry. RESULTS: Histological examination suggested that hepatocytes in rats fed with 2-FAA showed vacuole-like denaturation at the early stages, then dysplastic nodules appeared at the middle stage, and finally progressed to tubercles of cancerous nests. A tendency of increasing liver gp96 protein level was found from normal liver to precancerous to cancerous tissues during hepatoma development (P<0.01), and was in accordance with the changes in gp96 mRNA (P<0.05). CONCLUSION: HSP gp96 is involved in HCC development and its overexpression may be a useful marker for early diagnosis.

Cyclosporin A protects Balb/c mice from liver damage induced by superantigen SEB and D-GaIN

AIM To investigate the pathogenic effect ofSEB and D-GalN on liver and the protection ofcyclosporin A, the relationship between hepaticapoptosis and necrosis and the possiblemechanism of acute hepatic necrosis.METHODS After staphylococcal enterotoxin B(SEB ) mixed with D--galactosamine (D-GaiN )were injected intraperitoneally into Balb/c miceand those previously treated with cyclosporin A,blood samples were collected and livers wereisolated at 2, 6, 12 and 24 h. Patterns othepatocellular death were studiedmorphologically and biochemically, circulatingcytokines (TNF-a, IFN--y ) and mice mortalitywithin 24h was assessed.RESU’LTS The SEB could induce the typicalapoptotic changes of hepatocytes, the D-GaiNcould induce hepatocytes apoptosis anddegeneration at the same time, and the micehaving received the SEB + D-GaiN injectionsdeveloped apoptosis at 2 and 6 h, but after 12 hhepatocytes were characterized by severein jury, whereas all the examinations in thecyclosporin A treated mice were normal.CONCLUSION Hepatic cell apoptosis might berelated to necrosis, and massive hepatocyteapoptosis is likely the initiating step of acutehepatic necrosis in mice. The effects induced bySEB and D--GaiN on hepatocytes might bemediated by T cells, and could be prevented bycyclosporin A.

Interferon and lamivudine combination therapy versus lamivudine monotherapy for hepatitis B e antigen-negative hepatitis B treatment:a meta-analysis of randomized controlled trials

BACKGROUND: It has been demonstrated that only a minority of patients with hepatitis B e antigen (HBeAg) negative chronic hepatitis B (CHB) obtain a sustained response after either interferon (IFN) or nucleos (t)ide analogue monotherapy. Therefore, combination therapy of drugs with synergistic antiviral effects was proposed to have a sustained response in these patients. We compared the effect and safety of lamivudine monotherapy and its combination with IFN including conventional interferon (CON-IFN) and pegylated interferon (PEG-IFN) for HBeAg-negative CHB patients. DATA SOURCES: A group of three independent reviewers identified 9 eligible randomized controlled trials through electronic searches (MEDLINE, OVID, EMBASE, the Cochrane Library Clinical Trials Registry, and the Chinese Medical Database), manual searches, and contact with experts. Sustained virological and biochemical responses were defined as primary efficacy measures. We performed quantitative meta-analyses to assess differences between CON-IFN plus lamivudine combination and lamivudine monotherapy groups. RESULTS: No greater sustained virological and biochemical rates were found in patients receiving CON-IFN/lamivudine combination therapy [29.1% vs. 26.7%, odds ratio (OR)=0.98, 95% confidence interval (CI) 0.65-1.50, P=0.94, and 41.8% vs. 40.3%, OR=1.13, 95% CI 0.78-1.65, P=0.51, respectively],though a reduced YMDD mutation rate was achieved in the combination group [8.39% vs. 30.0%, OR=0.16, 95% CI 0.076-0.33, P<0.001]. However, data from one PEG-IFN trial showed greater sustained virological and biochemical rates in patients receiving combination therapy [response rate 19.5% vs. 6.6%, OR=3.42, 95% CI 1.71-6.84, P<0.001 and 60.0% vs. 44.2%, OR=1.88, 95% CI 1.23-2.85, P=0.003, respectively]. CONCLUSIONS: Addition of CON-IFN to lamivudine did not improve treatment efficacy but suppressed YMDD mutation by lamivudine. Combination of PEG-IFN and lamivudine might increase the sustained response, and further clinical trials are needed for confirmation.

Classification,diagnosis and treatment status of pulmonary hypertension from 2012 to 2019:a single center study in Yunnan province

Objective To analyze the classification,diagnosis and treatment status of patients with pulmonary hypertension(PH) in Yunnan province.Methods Hospitalized patients with PH at Yan’an Affiliated Hospital of Kunming Medical University from January 2012 to December 2019 were enrolled.

Expressions of programmed death-1 and programmed death ligand on the surface of peripheral blood lymphocytes in patients with tuberculosis

Objective To describe the expressions of programmed death-1(PD-1)and its ligand PD-L1 on the surface of peripheral blood lymphocytes in patients with tuberculosis.Methods A total of 77 cases of pulmonary tuberculosis were recruited,of which 27 were single infection,41 were coincident with bacterial or fungal infection and 9 patients with diabetes millitus.

Peripheral T and B lymphocytes in patients with occupational medicamentosa-like dermatitis due to trichloroethylene

Objective To investigate the changes in liver function and peripheral regulatory lymphocytes before and after treatment in patients with occupational medicamentosa-like dermatitis due to trichloroethylene (OMDT).

Completion rates of bundle treatment among patients with sepsis shock in intensive care departments of hospitals in Jiangsu Province from 2016 to 2020 years

Objective To study completion rates of 3 h and 6 h bundle treatment among patients with septic shock in intensive care units(ICUs)of hospitals in Jiangsu Province from 2016 to 2020,using data from the Jiangsu Provincial Intensive Care Medical Quality Control Center.Methods Current approaches and factors affecting treatment completion were assessed.The completion rates of 3 h and 6 h bundle treatment in ICUs of all medical units in Jiangsu Province and in ICUs of hospitals of different levels were recorded.

Safety and efficacy ofthe early administration of levosimendan in patients with acute non-ST-segment elevation myocardial infarction and elevated NT-proBNP levels: An Early Management Strategy of Acute Heart Failure (EMS-AHF)

Objective To investigate the safety and efficacy of treating patients with acute non-ST-segment elevation myocardial infarction(NSTEMI)and elevated levels of N-terminal pro-hormone B-type natriuretic peptide(NTproBNP)with levosimendan within 24 hours of first medical contact(FMC).

奶牛源葡萄球菌tetK基因碱基及氨基酸序列差异的功能分析

为探究奶牛源葡萄球菌中部分四环素耐药基因tetK不产生耐药性的原因,采用平行试验对四环素抗性菌株(MP00159)与四环素敏感菌株(MP00109)所携带的tetK基因和tetK′基因的碱基及氨基酸序列差异进行分析;随后将两者分别克隆至表达载体pET-32a(+)后导入大肠埃希菌感受态细胞BL21(DE3)内,比较转化前后对四环素的耐药性。结果表明:四环素敏感菌株携带的tetK′基因与抗性菌株的tetK基因存在5个碱基位点差异,对应的氨基酸出现4个差异;转入敏感菌株tetK′基因的BL21仍表现对四环素敏感,转入抗性菌株tetK基因的BL21表现了四环素抗性。由此推测发生突变的碱基位点是决定tetK耐药表型的关键位点。

Gene mapping of a new coat mutation in mouse

Gene mapping of a mouse coat mutation has been investigated. First, 100 10-bp random primers were used to amplify DNA, but the mutation could not be located by this method because there were no correlation between the amplified products and coat phenotypes. Second, by using ldh1 , Car2, Mup1 , Pgb1 , Hbb, Es1O, Es1, Mod1, Gdc1 , Ce2, Es3 as genetic markers, linkage test crosses (two-point test) consisting of intercrossing uncovered BALB/c mice (homozygotes) to CBA/N and C57BL/6 mice with normal hair and backcrossing the heterozygotes of the F1 to the uncovered BALB/c mice were made. It was soon evident that the mutation was linked to Es3 on chromosome 11. Furthermore, three-point test was made by using Es3 and D11 Mit8 (a microsatellite DNA) as genetic markers. The result showed that the mutation was linked to Es3 with the percentage recombination of (7.89 ± 2.19) %, and linked to D11Mit8 with the percentage recombination of (26.30 ± 3.57)%. The percentage recombination between Es3 and D11Mit8 was

Analysis of the results of Treponema Pallidum DNA in serum and vaginal fluid of patients with latent syphilis before and after treatment

Objective To investigate the infectivity of serum and vaginal fluid in early cases and late latent cases of syphilis.Methods From January 2015 to December 2015,sixty-eight female patients with latent syphilis were enrolled in the outpatient clinic of Ningbo Women and Children's Hospital,including 38 early cases and 30 late cases.Tolulized red unheated serum test(TRUST)was detected by using kits and Treponema Pallidum DNA(TP-DNA)was detected by polymerase chain reaction.TP-DNA levels in serum and vaginal fluid were compared before and after benzathine penicillin treatment in patients with early and late latent syphilis.Chi square test was performed for count data analysis.Results TRUST negative conversion cases in early latent syphilis after 3,6 and 12 months of treatment were 11(28.9%),16(42.1%)and 27(71.0%),respectively.TRUST negative conversion cases in late latent syphilis after 3,6 and 12 months of treatment were 6(20.0%),12(40.0%)and 14(46.7%),respectively.There were no statistical differences in TRUST negative conversion rates after 3 months and 6 months after treatment between early cases and late cases(X^2=0.716 and 0.031,respectively,both P>0.05),while those after 12 months of treatment between early cases and late cases were statistically different(X^2=4.164,P<0.05).The vaginal fluid TP-DNA positive cases in early syphilis before and after 3,6,and 12 months of treatment were 4(10.5%),0,1(2.63%)and 1(2.63%),respectively,with no statis-tical difference(X^2=6.247,P>0.05).The serum TP-DNA positive cases in early syphilis before and after 3,6,and 12 months of treatment were 18(47.3%),4(10.5%),5(13.2%)and 5(13.2%),respectively,with statistical difference(X^2=21.217,P<0.05).The vaginal fluid TP-DNA positive cases in late syphilis before and after 3,6,and 12 months of treatment were 2(6.7%),0,0 and 1(3.3%),respectively,with no statistical difference(X^2=3.761,P>0.05).The serum TP-DNA positive cases in late syphilis before and after 3,6,and 12 months of treatment were 8(26.7%),1(3.3%),1(3.3%)and 1(3.3%),respectively,with a statistical difference(X^2=14.712,P<0.05).Conclusion Standard treatment can reduce the level of TP in serum and vaginal fluid of latent syphilis women and improve the negative conversion rate of TRUST.Late latent syphilis is easier to create sero-resistance than early cases.The early cases have stronger infectivity than late cases before standard treatment.

The correlation between free fatty acid and insulin resistance in ischemic stroke patients

Objective To explore the level of free fatty acid (FFA) in ischemic stroke (IS) patients and its correlation with insulin resistance (IR) .Methods This study was case-control study.Patients who were diagnosed with IS were followed up from June,2011 to September,2012in Tianjin Third Central Hospital.The 180 IS patients were divied into large infarct (57) ,middle infarct (63) and small infarct (60) according to the area of infarct.At the same time,60 healthy persons were selected as con-

Establishment and preliminary application of Dengue virus envelope domain Ⅲ IgG antibody capture enzyme-linked immuno-absorbent assay

Objective To establish a highly sensitive and specific assay to detect Dengue virus(DENV) envelope protein domainⅢ(EDⅢ) IgG antibody,and to explore its value in the diagnosis and seroepidemiological survey of

Dynamic interferon-inducible protein-10expressions in the peripheral blood mononuclear cells and its prognostic value in patients with hepatitis B virus related acute-on-chronic liver failure

Objective To investigate the predictive value of dynamic changes of interferon-inducible protein-10(IP-10)expression in the peripheral blood mononuclear cells and the model for end-stage liver disease(MELD)scores for short-term mortality in hepatitis B virus related acute-on-chronic liver failure(HBV-ACLF)patients.Methods Eighty patients with HBV-ACLF admitted to the Affiliated Hospital of Hubei College of Arts and Sciences from October 2013 to August 2015 were selected.During 3 months of follow-up,33 patients died and 47 survived.The expression level of IP-10 and MELD score of two groups were measured at admission and 1 week and 2 weeks after treatment.The mean values between the two groups were compared.Accuracy of predicting short-term mortality was performed by area under receiver operating characteristic curve(AUC).Multivariate logistic regression analysis and Kaplan-Meier survival curve were used to analyze the effect of IP-10 expression and MELD score on the mortality of HBV-ACLF patients.Results The expressions of IP-10 at admission and at week 1 and 2 after treatment in the death group were 1.095±0.202,1.071±0.181,and 1.078±0.198,respectively,those in the survival group were 0.894±0.181,0.770±0.153,and 0.732±0.137,respectively,which were significantly different(t=4.66,8.02 and 9.27,respectively,all P<0.01).The MELD scores at admission and at week 1 and 2 after treatment in the death group were 26.70±5.50,27.39±6.24,and 28.64±6.44,respectively,those in the survival group were 23.89±4.41,21.57±4.68,and 18.87±3.92,respectively,which were significantly different(t=2.53,4.77 and 8.42,respectively,all P<0.01).Analysis of variance showed that the MELD score and IP-10 expression in the survival group at admission were significantly higher than those at week 1 and week 2 after treatment(F=13.464 and 15.711,respectively,both P<0.01),while there were no significant differences in the death group(F=0.129 and 0.864,respectively,both P>0.05).The AUC of IP-10 at week 2 after treatment was 0.935,that of MELD score was 0.903(Z=0.788,P=0.045),while there was no significant difference of AUC between week 1 and week 2(0.935 vs 0.909,Z=0.640,P>0.05).In addition,the AUC of IP-10 level at week 1 and MELD score at week 2 after treatment showed no significant difference(0.909 vs 0.903,Z=0.133,P>0.05).Logistic multivariate regression analysis showed that IP-10≥0.902 at week 1,MELD≥22.5 and IP-10≥0.846 at week 2 were independent risk factors for death(OR=11.29,6.60,and 15.27,respectively;95% CI=1.06-119.74,1.27-34.26,and 1.39-167.62,respectively;all P<0.05).Conclusion The dynamic monitor of both IP-10 levels and MELD scores may have greater value in predicting prognosis of patients with HBV-ACLF.

Clinical characteristics of viral pneumonia in patients with chronic obstructive pulmonary disease

Objective To study the clinical and etiological characteristics of viral pneumonia in patients with chronic obstructive pulmonary disease(VP-COPD),and toidentify the risk factors associated with poor prognosis.Methods From August 1,2017 to August 1,2019,totally 235 patients in a general hospital in Beijing were prospectively enrolled in this research,and all patients were diagnosed with viral pneumonia by imaging and etiology.

Study on sperm damage caused by trichloroethylene in male rats

Objective To study in vitro sperm damage caused by trichloroethylene in male rats.Methods Sperms of Sprague-Dawley(SD)rats were collected 4 hours after being contaminated by trichloroethylene of 0,2,4,6,8,and 10 mmol/L in vitro.Giemsa staining was performed to observe the morphological changes of sperms,and flow cytometer was used to detect the changes in mitochondrial membrane potential.Results The sperm motilities in6,8,and 10 mmol/L trichloroethylene groups decreased

Expression of ICAT and Wnt signaling-related proteins in the monocytic differentiation of HL-60 cells induced by a new steroidal drug NSC67657

Objective To investigate the expression of mRNA and proteins ofβ-catenin,TCF-4(ICAT)and Wnt signaling pathway-related genes in the monocytic differentiation of acute myeloid leukemia HL-60 cells induced by a new steroidal drug NSC67657.Methods Wright’s staining andα-NBE staining were used to observe the differentiation of HL-60 cells after 5 days of

Clinical characteristics of patients with acute pulmonary embolism in high altitude area of Yunnan province in China

Objective To analyze the clinical features of patients with acute pulmonary embolism (APE) living in high altitude area of Yunnan province.Methods This was a cross-sectional retrospective study. APE patients,hospitalized in our hospital between January 2017 and December 2019, were included. The selected patients were divided into low-risk group, medium-risk group and high-risk group according to risk stratification.

Changes in FOXO1 expression in β-cells during the progression of type 2 diabetes mellitus and aging

Objective To investigate the changes of β-cell dedifferentiation in type 2 diabetes mellitus(T2DM) and aging subjects.Methods Using pancreatic samples from 12 prediabetic individuals,21 type 2 diabetic patients,and 27 control,the level of β-cell dedifferentiation was assessed by immunofluorescence staining for FOXO1 and insulin expression.