Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and e...Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal antibody or peptides that are displayed on the surface of the phages can be occurred through biopanning process. In biopanning process phage library is incubated with antigen and particular phages can be identified and isolated. Increasing the stringency in the biopanning rounds can be help to select phages with high affinity and specificity. Here, we describe an overview of phage display application with focusing on monoclonal antibody production and epitope mapping.展开更多
Asthma is the most common chronic disease among children, and its incidences are often imminent among elementary schoolchildren. This study aimed to examine the prevalence of asthma symptoms in Golestan schoolchildren...Asthma is the most common chronic disease among children, and its incidences are often imminent among elementary schoolchildren. This study aimed to examine the prevalence of asthma symptoms in Golestan schoolchildren aged 6-7 and 13-14 years in Northeast Iran. The prevalence rate was compared according to age group （aged 6-7 years vs. aged 13-14 years） and gender （male vs. female）. In this cross-sectional study, 1706 Iranian schoolchildren aged 6-7 and 13-14 years in Golestan Province were enrolled. Participants completed questionnaires between February and July 2014. Asthma symptoms were assessed using the questionnaire of the International Study of Asthma and Allergies in Childhood protocol in Persian. The logistic regression model was used to estimate the odds ratios （ORs） and 95% confidence intervals （CIs） of the asthma symptoms for each of the gender and age groups. The prevalence rates of ＂current asthma＂ symptoms and ＂asthma ever＂ in all the children were estimated as 9.5%and 7.5%, respectively. The prevalence of asthma （＂asthma ever＂ and ＂wheezing in the past 12 months＂） in junior high schoolchildren （aged 13-14 years） is higher than that in elementary schoolchildren （aged 6-7 years） （P 〈 0.05）. The prevalence of the severity of wheezing in girls is lower than that in boys （OR = 1.7, 95%CI = 1.06-2.96, P = 0.02）. Asthma is still a major public health problem. This study shows that the prevalence of the asthma symptoms in boys is lower than that in girls in both age groups, and the severity of asthma in girls is higher than that in boys aged 13-14 years.展开更多
文摘Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal antibody or peptides that are displayed on the surface of the phages can be occurred through biopanning process. In biopanning process phage library is incubated with antigen and particular phages can be identified and isolated. Increasing the stringency in the biopanning rounds can be help to select phages with high affinity and specificity. Here, we describe an overview of phage display application with focusing on monoclonal antibody production and epitope mapping.
文摘Asthma is the most common chronic disease among children, and its incidences are often imminent among elementary schoolchildren. This study aimed to examine the prevalence of asthma symptoms in Golestan schoolchildren aged 6-7 and 13-14 years in Northeast Iran. The prevalence rate was compared according to age group （aged 6-7 years vs. aged 13-14 years） and gender （male vs. female）. In this cross-sectional study, 1706 Iranian schoolchildren aged 6-7 and 13-14 years in Golestan Province were enrolled. Participants completed questionnaires between February and July 2014. Asthma symptoms were assessed using the questionnaire of the International Study of Asthma and Allergies in Childhood protocol in Persian. The logistic regression model was used to estimate the odds ratios （ORs） and 95% confidence intervals （CIs） of the asthma symptoms for each of the gender and age groups. The prevalence rates of ＂current asthma＂ symptoms and ＂asthma ever＂ in all the children were estimated as 9.5%and 7.5%, respectively. The prevalence of asthma （＂asthma ever＂ and ＂wheezing in the past 12 months＂） in junior high schoolchildren （aged 13-14 years） is higher than that in elementary schoolchildren （aged 6-7 years） （P 〈 0.05）. The prevalence of the severity of wheezing in girls is lower than that in boys （OR = 1.7, 95%CI = 1.06-2.96, P = 0.02）. Asthma is still a major public health problem. This study shows that the prevalence of the asthma symptoms in boys is lower than that in girls in both age groups, and the severity of asthma in girls is higher than that in boys aged 13-14 years.
