Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbo...Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbone.At the 1st,2nd,4th,6th,8th and 12th week after the operation, they were examined by X-ray and ultrasound,respectively.All detection results were scored according to a generally accepted standard.Spearman rank correlation analysis was conducted to explore the relationship between the results of the two inspection methods.Results:In each healing stage,the results of the ultrasonic inspection were basically consistent with those of the X-ray examination,as supported by a Spearman rank correlation coefficient of 0.892(P【0.001). Conclusions:Non-invasive ultrasonic inspection can be used instead of X-ray examination to monitor and diagnose fracture healing.展开更多
The inflammasome is a multiprotein oligomer in the cell cytoplasm and is part of the innate immune system.It plays a crucial role in the pathological process of noise-induced hearing loss(NIHL).However,the mechanisms ...The inflammasome is a multiprotein oligomer in the cell cytoplasm and is part of the innate immune system.It plays a crucial role in the pathological process of noise-induced hearing loss(NIHL).However,the mechanisms of NLR family pyrin domain containing 3(NLRP3)inflammasome activation in NIHL have not been clearly demonstrated.In this study,miniature pigs were exposed to white noise at 120 dB(A)and auditory brainstem response measurements were used to measure their hearing function.Immunofluorescence staining,confocal laser scanning microscopy,western blot assay,and quantitative reverse transcription-polymerase chain reaction were used to analyze inflammasome-related protein distribution and expression.NLRP3,interleukin-1β,interleukin-18,and cleaved-caspase-1 were highly expressed in the cochlea after 120 dB(A)white noise exposure.Our findings suggest that NLRP3-inflammasomes in the cochlea may be activated after acoustic trauma,which may be an important mechanism of noise-induced hearing loss.展开更多
Neural crest(NC)is the primitive neural structure in embryonic stage,which develops from ectodermal neural plate cells and epithelial cells.When the neural fold forms into neural tube,neural crest also forms a cord li...Neural crest(NC)is the primitive neural structure in embryonic stage,which develops from ectodermal neural plate cells and epithelial cells.When the neural fold forms into neural tube,neural crest also forms a cord like structure above the neural tube and below the ectoderm.Neural crest cells(NCC)have strong migration and proliferation abilities.A number of tissue cells differentiate from neural crest cells,such as melanocytes,central and peripheral neurons,glial cells,craniofacial cells,osteoblasts,chondrocytes and smooth muscle cells.The migration and differentiation of neural crest cells are regulated by a gene network where a variety of genes,transcriptional factors,signal pathways and growth factors are involved.展开更多
基金Supported by the Research and Development Project of Scientific and Technological Industry of Guangdong Province(2011B080701053)
文摘Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbone.At the 1st,2nd,4th,6th,8th and 12th week after the operation, they were examined by X-ray and ultrasound,respectively.All detection results were scored according to a generally accepted standard.Spearman rank correlation analysis was conducted to explore the relationship between the results of the two inspection methods.Results:In each healing stage,the results of the ultrasonic inspection were basically consistent with those of the X-ray examination,as supported by a Spearman rank correlation coefficient of 0.892(P【0.001). Conclusions:Non-invasive ultrasonic inspection can be used instead of X-ray examination to monitor and diagnose fracture healing.
基金supported by the National Key Research and Development Program of China, No.2020YFC2005200(to WWG and WJH)the National Nature Science Foundation of China, Nos.81770992(to NS and WJH), 81970897(to WWG)+1 种基金Health and Family Planning System Research Project of Shenzhen Municipality, No.SZXJ2018079(to YYY)Shenzhen Sanming Project, No.SZSM201612076(to YYY)
文摘The inflammasome is a multiprotein oligomer in the cell cytoplasm and is part of the innate immune system.It plays a crucial role in the pathological process of noise-induced hearing loss(NIHL).However,the mechanisms of NLR family pyrin domain containing 3(NLRP3)inflammasome activation in NIHL have not been clearly demonstrated.In this study,miniature pigs were exposed to white noise at 120 dB(A)and auditory brainstem response measurements were used to measure their hearing function.Immunofluorescence staining,confocal laser scanning microscopy,western blot assay,and quantitative reverse transcription-polymerase chain reaction were used to analyze inflammasome-related protein distribution and expression.NLRP3,interleukin-1β,interleukin-18,and cleaved-caspase-1 were highly expressed in the cochlea after 120 dB(A)white noise exposure.Our findings suggest that NLRP3-inflammasomes in the cochlea may be activated after acoustic trauma,which may be an important mechanism of noise-induced hearing loss.
文摘Neural crest(NC)is the primitive neural structure in embryonic stage,which develops from ectodermal neural plate cells and epithelial cells.When the neural fold forms into neural tube,neural crest also forms a cord like structure above the neural tube and below the ectoderm.Neural crest cells(NCC)have strong migration and proliferation abilities.A number of tissue cells differentiate from neural crest cells,such as melanocytes,central and peripheral neurons,glial cells,craniofacial cells,osteoblasts,chondrocytes and smooth muscle cells.The migration and differentiation of neural crest cells are regulated by a gene network where a variety of genes,transcriptional factors,signal pathways and growth factors are involved.
